Imaging system for progression of gastrointestinal cancer and its application to anti-metastasis therapy

• Project/Area Number: 17K10589
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Okayama University
• Principal Investigator: Nishizaki Masahiko 岡山大学, 大学病院, 講師 (30379789)
• Co-Investigator(Kenkyū-buntansha): 田澤 大 岡山大学, 大学病院, 准教授 (90415513)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 大腸癌 / 転移 / 蛍光イメージング / EMT / アスピリン / 消化器癌 / 抗転移薬剤 / 蛍光発現ウイルス

Outline of Final Research Achievements

Patients with metastatic and disseminated gastrointestinal cancers show poor prognosis and the development of anti-metastasis therapy to prevent cancer metastasis and dissemination is an important issue. Here, we developed a drug screening system using colorectal cancer (CRC) cells with fluorescence probe for epithelial-mesenchymal transition (EMT). Anti-inflammatory drug aspirin inhibited inflammatory cytokine-induced EMT and prevented peritoneal dissemination of CRC cells. In the future, we need to evaluate the therapeutic effect of aspirin in the metastasis and dissemination of non-CRC cells.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、抗炎症薬剤として大腸癌の発生予防に効果があると考えられてきたアスピリンが炎症性サイトカインによるEMT誘導に対する阻害作用を介して大腸癌の腹膜播種の予防にも効果を発揮する新たな治療戦略となる可能性を示唆している。転移や播種を有する消化器癌患者の予後は不良であり、転移を予防する抗転移療法の開発研究は消化器癌患者の生命予後の改善につながるため社会的意義は大きい。

Research Products

• [Journal Article] Visualization of epithelial-mesenchymal transition in an inflammatory microenvironment?colorectal cancer network (2019)
  • Author(s): Ieda Takeshi、Tazawa Hiroshi、Okabayashi Hiroki、Yano Shuya、Shigeyasu Kunitoshi、Kuroda Shinji、Ohara Toshiaki、Noma Kazuhiro、Kishimoto Hiroyuki、Nishizaki Masahiko、Kagawa Shunsuke、Shirakawa Yasuhiro、Saitou Takashi、Imamura Takeshi、Fujiwara Toshiyoshi
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 16378-16378
  • DOI: 10.1038/s41598-019-52816-z
  • Peer Reviewed / Open Access
• [Presentation] 解熱剤のアスピリンはEMT阻害作用を介して大腸がんの腹膜播種を抑制する (2019)
  • Author(s): 岡林弘樹、田澤 大、家田偉史、矢野修也、菊地覚次、黒田新士、西 崎正彦、香川俊輔、今村健志、藤原俊義
  • Organizer: 第78回日本癌学会学術総会
• [Presentation] 解熱剤のアスピリンはEMT阻害によって大腸がん腹膜播種を抑制する (2019)
  • Author(s): 岡林弘樹、田澤 大、家田偉史、矢野修也、菊地覚次、黒田新士、西 崎正彦、香川俊輔、今村健志、藤原俊義
  • Organizer: 第32回日本バイオセラピィ学会学術集会総会
• [Presentation] Visualization of epithelial-mesenchymal transition in inflammatory microenvironment-colorectal cancer network in vitro and in vivo. (2018)
  • Author(s): Tazawa H, Ieda T, Yano S, Kikuchi S, Kuroda S, Ohara T, Noma K, Kishimoto H, Nishizaki M, Kagawa S, Saitou T, Imamura T, Fujiwara T.
  • Organizer: 109th Annual Meeting of the American Association for Cancer Research
  • Int'l Joint Research
• [Presentation] 炎症性微小環境―大腸がんクロストークにおけるEMTイメージング (2018)
  • Author(s): 田澤 大、家田偉史、矢野修也、重安邦俊、黒田新士、大原利章、野間和広、岸本浩行、西崎正彦、香川俊輔、白川靖博、齋藤 卓、今村健志、藤原俊義
  • Organizer: 第77回日本癌学会学術総会
  • Int'l Joint Research
• [Presentation] Fluorescence-guided spatiotemporal dynamics of epithelial-mesenchymal transition under inflammatory microenvironment during colorectal cancer progression. (2017)
  • Author(s): Ieda T, Tazawa H, Kikuchi S, Kuroda S, Ohara T, Noma K, Kishimoto H, Nagasaka T, Nishizaki M, Kagawa S, Fujiwara T.
  • Organizer: 108th Annual Meeting of the American Association for Cancer Research
  • Int'l Joint Research
• [Presentation] EMT-がん微小環境ネットワークの蛍光生細胞イメージングシステム (2017)
  • Author(s): 家田偉史、田澤 大、菊地覚次、黒田新士、大原利章、野間和広、岸本浩行、永坂岳司、西崎正彦、香川俊輔、今村健志、藤原俊義
  • Organizer: 第76回日本癌学会学術総会