Neutrophil extracellular traps in the development of peritoneal metastasis
Project/Area Number |
17K10606
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Jichi Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
北山 丈二 自治医科大学, 医学部, 教授 (20251308)
山口 博紀 自治医科大学, 医学部, 教授 (20376445)
大澤 英之 自治医科大学, 医学部, 講師 (60458271)
倉科 憲太郎 自治医科大学, 医学部, 講師 (70382900)
|
Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 好中球細胞外トラップ / 腹膜播種 / 胃癌 / 接着 / DNAse 1 / 低比重好中球 / 好中球 / 細胞外トラップ / 腹膜転移 / 癌 / 消化器外科学 / 胃十二指腸外科学 |
Outline of Final Research Achievements |
The number of CD66b (+) low density neutrophils (LDN) with mature phenotype was markedly elevated in postoperative as compared with preoperative lavages in patients who underwent laparotomy due to gastric cancer. Short term culture of the purified LDN produced many threadlike structures positive for SYTOX, suggesting the NETs formation. Human gastric cancer cells, MKN45, OCUM-1 and NUGC-4, were selectively attached on the NETs, which was abolished by DNAse I. Intraperitoneal co-transfer of the LDN with MKN45 in nude mice strongly augments the metastasis formation on peritoneum, which was strongly suppressed by the following IP administration of DNAse I. Many NETs-like structures were detected on the surface of human omental tissue resected by gastrectomy. NETs on peritoneal surface can assist the clustering and growth of free tumor cells disseminated in abdomen. Disruption of the NETs by DNAse might be useful to prevent the peritoneal recurrence after abdominal surgery.
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Academic Significance and Societal Importance of the Research Achievements |
腹膜播種の成立における腹腔内滲出好中球とNETsの役割を明らかにすると共に、DNAse1投与によるNETsの分解が播種再発の予防につながる可能性があることを見出した。今後の腹膜播種治療における新たな方法論を導く糸口になる成果を得た。
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Report
(4 results)
Research Products
(19 results)
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[Presentation] Neutrophil Extracellular Traps(NETs) on Peritoneal Surface After Abdominal Surgery may enhance peritoeal recurrence.2019
Author(s)
Rihito Kanamaru., Hideyuki Ohzawa., Kazuya Takahashi., Shiro Matsumoto., Takashi Ui., Hidenori Haruta., Kentaro Kurashina., Shin Saito., Hironori Yamaguchi., Yoshinori Hosoya., Naohiro Sata., Joji Kitayama.
Organizer
14th International Symposium on Regional Cancer Therapies
Related Report
Int'l Joint Research
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[Presentation] Neutrophil extracellular traps(NETs) on peritoneal surface after open abdominal surgery.2018
Author(s)
Kanamaru Rihito, Hideyuki Ohzawa, Kazuya Takahashi, Shiro Matsumoto, Hiedenori Haruta, Kentaro Kurashina, Hironori Yamaguchi, Yasunaru Sakuma, Hisanaga Horie, Yoshinori Hosoya, Joji Kitayama and Naohiro Sata
Organizer
第17回自治医科大学シンポジウム
Related Report
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[Presentation] 好中球細胞外トラップ(NET’s)による開腹術後腹膜再発促進機構(Neutrophil extracellular traps(NETs) on postoperative peritoneal surface may support the tumor recurrence on peritoneum.)2018
Author(s)
金丸理人, 大澤英之, 高橋和也, 松本志郎, 春田英律, 倉科憲太郎, 山口博紀, 佐久間康成, 堀江久永, 細谷好則, 佐田尚宏, 北山丈二
Organizer
第77回日本癌学会総会記事
Related Report
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[Presentation] 開腹手術後の腹腔内滲出好中球由来細胞外トラップ(NETs)の臨床的意義2018
Author(s)
金丸理人, 大澤英之, 松本志郎, 宇井崇, 春田英律, 宮戸秀世, 倉科憲太郎, 斉藤心, 山口博紀, 佐久間康成, 堀江久永, 細谷好則, 佐田 尚宏, 北山 丈二
Organizer
第39回癌免疫外科研究会
Related Report
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[Presentation] 消化器癌手術患者における末梢血中低比重好中球(Low Density Granulocyte)の術中推移2018
Author(s)
熊谷祐子, 金丸理人, 津久井秀則, 大澤英之, 倉科憲太郎, 鯉沼広治, 佐久間康成, 堀江久永, 細谷好則, 佐田尚宏, 北山丈二
Organizer
第118回日本外科学会定期学術集会
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[Presentation] 当院における漿膜浸潤胃癌R0切除症例における術中出血量と生命予後の関係2018
Author(s)
金丸理人, 大澤英之, 高橋和也, 松本志郎, 宇井崇, 春田英律, 倉科憲太郎, 宮戸秀世, 齋藤心, 山口博紀, 佐久間康成, 堀江久永, 細谷好則, 佐田尚宏, 北山丈二
Organizer
第90回日本胃癌学会総会
Related Report
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[Presentation] Neutrophil extracellular traps (NETs) in abdominal cavity have supportive roles on postoperative development of peritoneal metastasis.2017
Author(s)
Rihito Kanamaru, Hidenori Tsukui, Shin Saito, Kentaro Kurashina, Takashi Ui, Shiro Matsumoto, Hironori Yamaguchi, Yasunaru Sakuma, Hisanaga Horie, Yoshinori Hosoya, Naohiro Sata, Joji Kitayama.
Organizer
AACR 2017 Annual Meeting
Related Report
Int'l Joint Research
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[Presentation] 胃癌腹膜再発における開腹術後の腹腔内滲出液中low density granulocytes(LDG)の意義2017
Author(s)
金丸 理人,津久井 秀則,斉藤 心,倉科 憲太郎,宇井 崇,春田 英律,松本 志郎,安部 望,高橋 和也,山口 博紀,佐久間 康成,堀江 久永,細谷 好則,佐田 尚宏,北山 丈二
Organizer
第117回日本外科学会定期学術集会
Related Report
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