Comprehensive analysis of plasma-related miRNA in gastric cancer for establishment of new therapeutic strategy and biomarker.

Research Project

Project/Area Number	17K10615
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Digestive surgery
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	Tsujiura Masahiro 京都府立医科大学, 医学(系)研究科(研究院), 特任助教 (60448735)
Co-Investigator(Kenkyū-buntansha)	大辻英吾京都府立医科大学, 医学(系)研究科(研究院), 教授 (20244600) 市川大輔山梨大学, 大学院総合研究部, 教授 (20347446) 小松周平京都府立医科大学, 医学(系)研究科(研究院), 特任講師 (40578978)
Project Period (FY)	2017-04-01 – 2020-03-31
Project Status	Completed (Fiscal Year 2019)
Budget Amount *help	¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000) Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	胃癌 / マイクロRNA / 間質 / 医歯薬学 / 消化器外科学
Outline of Final Research Achievements	Candidate microRNAs related to peri-cancerous stroma were investigated by comprehensive database analysis and array analysis in gastric cancer. Candidate microRNAs were further selected based on the results of the array analysis, considering both the comparison between the cancerous area and the peri-cancerous stroma, and the comparison between the peri-cancerous stroma and the non-cancerous stroma. A validation study was conducted using multiple tissue samples, but it is difficult to prove the adequacy probably due to the influence of diversity or heterogeneity in gastric cancer and stromal tissue. Preliminary analysis was additionally performed to study optimal approach for distinguishing the cancerous area, the peri-cancerous area, and the non-cancerous area, and extracting tissue samples.
Academic Significance and Societal Importance of the Research Achievements	癌の増殖・転移に関しては、癌細胞を取り巻く微小環境とマイクロRNAが注目されており、胃癌においても癌の発生・進展に周囲間質が重要である可能性がある。本研究では、胃癌組織や間質組織中の多様性・異質性・不均一性の影響があるのか、周囲間質に関連するマイクロRNAについての検証は困難であった。それらは、癌部・癌周囲間質・非癌部間質の区分方法や実際の組織抽出方法により、今後改善する可能性があると考えられた。