Identification of biomaker for predicting liver metastasis of colorectal cancer with CAGE analysis
Project/Area Number |
17K10652
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Juntendo University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
加藤 俊介 順天堂大学, 大学院医学研究科, 教授 (40312657)
茂櫛 薫 順天堂大学, 大学院医学研究科, 非常勤講師 (60569292)
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Project Period (FY) |
2017-04-01 – 2023-03-31
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Project Status |
Completed (Fiscal Year 2022)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 腫瘍マーカー / 大腸がん / 肝転移 / 転移予測マーカー / 癌遺伝子 / 採血診断 / 大腸癌 / バイオマーカー / CAGE法 / マイクロダイセクション / 転移メカニズム |
Outline of Final Research Achievements |
This study aims to develop a predictive marker for liver metastasis in colorectal cancer through blood sample diagnosis. With comprehensive gene expression analysis of the primary cancer sites, we have identified genes that show cancer-specific, metastatic case-specific, and liver metastasis-specific up- or down-regulation. These genes have been thoroughly examined and narrowed down to potential marker candidates. Based on expression analysis with blood samples drawn from patients before and repeatedly after the operation,a few genes whose levels were associated with liver metastasis were selected. These genes belong to the group of genes exhibiting cancer-specific sequence mutations. The relationship between these candidate genes and local recurrence or metastasis to other organs has been confirmed, and the selection of genes as predictive markers for liver metastasis is currently in progress.
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Academic Significance and Societal Importance of the Research Achievements |
本研究は, 大腸癌の肝転移特異的なゲノム変異に着目, 肝転移特異的変異配列をctDNAから検出・定量する. 一般的マーカー量増大からの悪性化予測ではなく「早期検出が治療選択のために特に重要」な「肝転移」をターゲットとする鋭敏なマーカー開発に目標をおくことは本研究の独自性であると考える.本研究では同時に,患者による多様性がみられる変異配列を個別診断マーカーとして作出・利用する方法の確立を目指す. 現在普及・拡大しつつある遺伝診断の結果から, 個別診断マーカーを取得, 個々の患者に利用するシステムの開発を目指す本研究は、時流に即しつつ独自性が高いものといえる。
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Report
(7 results)
Research Products
(41 results)
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[Journal Article] Usefulness of an IL-6 Semi-Quantification Kit in Patients with Perforative Peritonitis2021
Author(s)
Ichikawa R, Sugimoto K, Kure K, Honjo K, Takahashi R, Okazawa Y, Kawai M, Munakata S,Komiyama H, Sakamoto K et al..
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Journal Title
Juntendo Medical Journal
Volume: 67
Issue: 2
Pages: 158-164
DOI
NAID
ISSN
2187-9737, 2188-2126
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[Journal Article] Identification of MicroRNA 15b-3p as a Diagnostic Marker for Early Stage of Colorectal Cancer Through Comprehensive RNA Analysis2021
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Tsukamoto R, Hosoya M, Fukaya M, Yokoyama N, Kawano S, Sugimoto K, Takahashi M, Sakamoto K, Komiyama H,
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Juntendo Medical Journal
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Issue: 3
Pages: 272-281
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NAID
ISSN
2187-9737, 2188-2126
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[Journal Article] Antitumor effects of novel mAbs against cationic amino acid transporter 1 (CAT1) on human CRC with amplified CAT1 gene2021
Author(s)
Okita K, Hara Y, Okura H, Hayashi H, Sasaki Y, Masuko S, Kitadai E, Masuko K, Yoshimoto S, Komiyama H, Sakamoto K, Masuko T.et al
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Pages: 563-574
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[Journal Article] 大腸癌術後肝門部リンパ節転移による閉塞性黄疸に対して胆管ステント留置後に分子標的薬併用全身化学療法を施行した1例.2020
Author(s)
雨宮 浩太, 百瀬 裕隆, 青木 順, 岡澤 裕, 水越 幸輔, 河野 眞吾, 宗像 慎也, 石山 隼, 杉本 起一, 神山 博彦, 小見山 博光, 高橋 玄, 小島 豊, 奥澤 淳司, 坂本 一博:
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癌と化学療法
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[Journal Article] Japan society of clinical oncology/Japanese society of medical oncology-led clinical recommendations on the diagnosis and use of tropomyosin receptor kinase inhibitors in adult and pediatric patients with neurotrophic receptor tyrosine kinase fusion-positive advanced solid tumors, cooperated by the Japanese society of pediatric hematology/oncology.2020
Author(s)
Naito Y, Mishima S, Akagi K, Igarashi A, Ikeda M, Okano, S, Kato S, Takano T, Tsuchihara K, Terashima K, et al.
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Int J Clin Oncol
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[Journal Article] Metastatic seeding of human colon cancer cell clusters expressing the hybrid epithelial/mesenchymal state.2019
Author(s)
Mizukoshi K, Okazawa Y, Haeno H, Koyama Y, Sulidan K, Komiyama H, Saeki H, Ohtsuji N, Ito Y, Kojima Y, Goto M, Habu S, Hino O, Sakamoto K, Orimo A.
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Int J Cancer
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Issue: 9
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[Journal Article] Stromal fibroblasts induce metastatic tumor cell clusters via epithelial-mesenchymal plasticity.2019
Author(s)
Matsumura Y, Ito Y, Mezawa Y, Sulidan K, Daigo Y, Hiraga T, Mogushi K, Wali N, Suzuki H, Itoh T, Miyagi Y, Yokose T, Shimizu S, Takano A, Terao Y, Saeki H, Ozawa M, Abe M, Takeda S, Okumura K, Habu S, Hino O, Takeda K, Hamada M, Orimo A.
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Volume: 2(4):e201900425.
Issue: 4
Pages: 1-24
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Author(s)
Horimoto Y, Terao T, Tsutsumi Y, Tanabe M, Mogushi K, Hlaing MT, Sasaki R, Saeki H, Okazaki M, Sonoue H, Arakawa A, Saito M.
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Journal Title
Am J Surg Pathol
Volume: 43(9)
Issue: 9
Pages: 1221-1228
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[Journal Article] High-sensitivity detection of micrometastases generated by GFP lentivirus-transduced organoids cultured from a patient-derived colon tumor.2018
Author(s)
OkazawaY, Mizukoshi K, KoyamaY, Okubo S, Komiyama H, Kojima Y, Goto M, Habu S, Hino O, Sakamoto K, Orimo A.
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[Journal Article] Survival benefit of lymph node dissection in surgery for colon cancer in elderly patients:multicenter propensity score-matched study in Japan.2018
Author(s)
Takahashi M, Niitsu H, Sakamoto K, Hinoi T, Hattori M, Goto M, Bando H, Hazama S, Maeda K, Okita K, Horie H, Watanabe M; Japan Society of Laparoscopic Colorectal Surgery.
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Journal Title
Asian J Endosc Surg
Volume: 2018 Mar 30
Issue: 4
Pages: 1-9
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[Journal Article] High-sensitivity detection of micrometastases generated by GFP lentivirus-transduced organoidscultured from a patient-derived colon tumor2018
Author(s)
Okazawa Y, Mizukoshi K, Koyama Y, Okubo S, Komiyama H, Kojima Y, Goto M, Habu S, Hino O,Sakamoto K, Orimo A.
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[Journal Article] Molecular and clinical features of the TP53 signature gene expression profile in early-stage breast cancer.2018
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Yamaguchi S, Takahashi S, Mogushi K, Izumi Y, Nozaki Y, Nomizu T, Kakugawa Y, Ishida T, Ohuchi N, Ishioka C, Kato S.
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Oncotarget
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[Journal Article] Minimally Invasive Surgery for Colorectal Cancer2017
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Sakamoto K, Tsukamoto R, Kawano S, Kawai M, Niwa K, Ishiyama S, Sugimoto K, Kamiyama H, Komiyama H, Takahashi M, Kojima Y, Goto M, Okuzawa A, Tomiki Y:
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Journal Title
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Volume: 63
Issue: 5
Pages: 384-392
DOI
NAID
ISSN
2187-9737, 2188-2126
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[Journal Article] Propensity Score Analysis in the Comparison of Long-Term Outcomes for Locally Advanced Colon Cancer Between Laparoscopic Colectomy and Open Colectomy2017
Author(s)
Sugimoto K, Sakamoto K, Ichikawa R, Kure K, Honjo K, Ro H, Takahashi R, Kawano, Niwa K, Ishiyama S, Kamiyama H, Komiyama H, Takahashi M, Kojima Y, Goto M, Okuzawa A, Ishibiki Y, Tomiki Y
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Journal Title
Juntendo Medical Journal
Volume: 63
Issue: 4
Pages: 264-272
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2187-9737, 2188-2126
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[Journal Article] Immune-complex level of cofilin-1 in sera is associated with cancer progression and poor prognosis in pancreatic cancer.2017
Author(s)
Satoh M, Takano S, Sogawa K, Noda K, Yoshitomi H, Ishibashi M, Mogushi K, Takizawa H, Otsuka M, Shimizu H, Miyazaki M, Nomura F.
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Journal Title
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Volume: 印刷中
Issue: 4
Pages: 795-803
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[Presentation] 大腸癌における原発巣および血漿中遊離DNAのCHFR遺伝子メチル化測定の意義.2019
Author(s)
杉本起一, 百瀬裕隆, 岡澤 裕, 高橋里奈, 水越幸輔, 盧 尚志, 河合雅也, 宗像慎也, 神山博彦, 高橋 玄, 小島 豊, 冨木裕一, 福永 哲, 梶山美明, 坂本一博:
Organizer
第57回日本癌治療学会学術集会, 福岡.
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[Presentation] Identification of a novel diagnostic marker for colon cancer through comprehensive microRNA analysis.2017
Author(s)
Tsukamoto R, Komiyama H, Hosoya M, Kure K, Okazawa Y, Mizukoshi K, Fukaya M, Sugimoto K, Kamiyama H, Tomiki Y, Sakamoto K:
Organizer
第76回日本癌学会学術総会, 横浜
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[Presentation] 大腸癌同時性肝転移症例におけるGlasgow Prognostic Score (GPS) を用いた予後評価2017
Author(s)
杉本起一,市川亮介,高橋里奈,盧尚志,河野眞吾,丹羽浩一郎,石山 隼,神山博彦,小見山博光,高橋玄,小島豊,五藤倫敏,奥澤淳司,冨木裕一,吉本次郎,今村宏,石崎陽一,川崎誠治,福永哲,梶山美明,坂本一博.
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第117回日本外科学会定期学術集会, 横浜
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