• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Epithelial- Mesenchymal transition and genetic mutation in Lymph node metastasis in extrahepatic cholalngiocarcinoma

Research Project

Project/Area Number 17K10684
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionHokkaido University

Principal Investigator

Nakanishi Yoshitsugu  北海道大学, 大学病院, 特任助教 (40447058)

Co-Investigator(Kenkyū-buntansha) 平野 聡  北海道大学, 医学研究院, 教授 (50322813)
土川 貴裕  北海道大学, 大学病院, 講師 (50507572)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords肝外胆管癌 / 肝門領域胆管癌 / 遠位胆管癌 / EMT / リンパ節転移 / Tumor Budding / 遺伝子変異 / NGS / tumor budding / 肝門部領域胆管癌 / lymph node metastasis / MET / 消化器外科学 / 胆道外科学
Outline of Final Research Achievements

To clarify the mechanism of lymph node metastasis in extrahepatic cholangiocarcinoma (EHCC), we performed studies as follows: 1) the association between the number of tumor budding (TB) at the invasive area and clinicopathological factors, postoperative prognosis. 2)associations between the number of TB and expression of immunohistochemical staining of proteins associated with epithelial-mesenchymal transition (EMT). 3) gene mutations in primary tumor and lymph node metastasis by using next generation sequencer. Results. 1) There were significantly associations between high number grade of TB and invasive natural factors and adverse prognosis. 2) There was significantly correlation between high staining of EMT pattern and higher number of TB. 3) There was not characteristic mutation of lymph node metastasis. However, mutations in primary and lymph node were almost same.

Academic Significance and Societal Importance of the Research Achievements

1)の結果から癌細胞がTBの形態をとることが転移のプロセスで重要であると示唆された。また、TBはEMTの形態学的表現形であることが推測されたが、2)の結果はこれを強く支持する結果と言える。しかし、3)の結果からはリンパ節転移に特化した遺伝子変異は同定できなかったことから、EMTは遺伝子変異だけで説明できるものでないことが示唆された。さらに3)の結果から肝外胆管癌の遺伝子変異は症例ごとに異なっているが、転移巣においても原発と変異がほぼ同等であることから、症例ごとに原発巣における遺伝子変異を調べることにより、再発転移巣の治療ターゲットとなる遺伝子変異を推測することが将来的に可能になると考えられた。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] Impact of Tumour Budding Grade in 310 Patients Who Underwent Surgical Resection for Extrahepatic Cholangiocarcinoma.2019

    • Author(s)
      Ogino M, Nakanishi Y, Mitsuhashi T, Hatanaka Y, Amano T, Marukawa K, Nitta T, Ueno T, Ono M, Kuwabara S, Yamada T, Hirano S.
    • Journal Title

      Histopathology.

      Volume: 「印刷中」 Issue: 6 Pages: 861-872

    • DOI

      10.1111/his.13827

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 肝外胆管癌におけるtumor budding の臨床応用に向けた検討2019

    • Author(s)
      荻野真理子,中西喜嗣,三橋智子,畑中 豊,田中公貴,浅野賢道,野路武寛,倉島 庸,海老原祐磨,村上壮一,中村 透,土川貴裕,岡村圭祐,七戸俊明,平野 聡
    • Organizer
      日本外科学会定期学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 肝外胆管癌におけるtumor buddingの臨床応用に向けた検討2019

    • Author(s)
      荻野真理子, 中西喜嗣、三橋智子、畑中 豊、田中公貴、浅野賢道、倉島 庸、海老原裕磨、村上壮一、中村 透、土川貴裕、岡村圭祐、七戸俊明、平野 聡
    • Organizer
      第119回 日本外科学会定期学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] Impact of the quantity of isolated tumor buds for postoperative prognosis in patients with extrahepatic cholangiocarcinoma2018

    • Author(s)
      Mariko Ogino, Yoshistugu Nakanishi, Satoshi Hirano et al.
    • Organizer
      the 30 th meeting Japan society of Hepato-Biliary-Pancreas Surgery
    • Related Report
      2018 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2022-12-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi