| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Previous studies on arterial and venous grafts have shown that prostanoids may prevent graft failure due to intimal thickening. However, its mechanism has not yet been clarified. We hypothesized based on the embryological findings of prostanoid receptors that prostanoid receptors are expressed in vascular smooth muscle cells (VSMCs) dedifferentiated during the process of vascular remodeling, and these receptors are involved in positive remodeling of arterial and venous grafts. In a rat autologous vein implantation model, the expression of EP2 and IP (prostacyclin receptor) mRNAs and proteins during graft remodeling was investigated using quantitative polymerase chain reaction and western blotting, respectively. The localization of EP2 and IP was also investigated by immunohistochemistry. We demonstrated that EP2 and IP were expressed in dedifferentiated VSMCs during the remodeling process, and this result suggested that these receptors may be involved in graft remodeling.
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