splice varients of target moleclues in antibody therapies for lung cancer
Project/Area Number |
17K10796
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Respiratory surgery
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Research Institution | Kagoshima University |
Principal Investigator |
sato masami 鹿児島大学, 医歯学域医学系, 教授 (30250830)
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Co-Investigator(Kenkyū-buntansha) |
横枕 直哉 鹿児島大学, 医歯学総合研究科, 特任助教 (00418857)
狩集 弘太 鹿児島大学, 医歯学域鹿児島大学病院, 助教 (20648050)
大塚 綱志 鹿児島大学, 医歯学域鹿児島大学病院, 助教 (40464466)
青木 雅也 鹿児島大学, 医歯学域医学系, 助教 (50624996)
中村 好宏 鹿児島大学, 医歯学域鹿児島大学病院, 講師 (60381159)
永田 俊行 鹿児島大学, 医歯学域鹿児島大学病院, 助教 (70611763)
武田 亜矢 鹿児島大学, 医歯学総合研究科, 特任助教 (80794700)
|
Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 免疫学 / スプライスバリアント / PD-1/PD-L1 / 抗体療法 / Nivolumab / 外科臨床 / 外科 / 臨床 / マイクルアレイ |
Outline of Final Research Achievements |
The purpose of this study is to examine the molecular biological correlation between splice abnormalities and the therapeutic effects of target molecules, for lung cancer antibody treatment, so we examined the relationship between the therapeutic effects of anti-PD-1 antibody and anti-PD-L1 antibody, which have started to be used in actual clinical practices for lung cancer, along with splice variants of PD-1 and PDL-1.5 and 7 primer sets were contrived in order to discriminate 4 types of PD-1 splice variants and 7 types of PD-L1 splice variants, respectively.we examined the splice patterns by PCR using blood specimens before and after treatment of 29 patients who underwent antibody therapy for non-small cell lung cancer. The therapeutic effects of Nivolumab in the 29 cases included 1 case of CR, 6 cases of PR, 10 cases of SD, and 12 cases of PD. No characteristic variant was found in patients treated with anti-PD-1 antibody Nivolumab.
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Academic Significance and Societal Importance of the Research Achievements |
本研究は、進行非小細胞肺癌患者に対する高額な治療薬剤である抗PD-1抗体オプジーボの、効果的・効率的な治療効果予測因子を探索することが目的であった。本研究により、抗体療法の治療効果に、PD-1/PD-L1の選択的スプライシングが関与していないことが確認され、抗体療法の治療効果予測因子の探索において、今後の研究分野を限定することができたことは、学術的、社会的意義があった。
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Report
(4 results)
Research Products
(67 results)
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[Journal Article] MicroRNA-130b functions as an oncomiRNA in non-small cell lung cancer by targeting tissue inhibitor of metalloproteinase-22019
Author(s)
Hirono T, Jingushi K, Nagata T, Sato M, Minami K, Aoki M, Takeda AH, Umehara T, Egawa H, Nakatsuji Y, Kitae K, Ueda Y, Hase H, Yamamoto M, Shinsato Y, Kawahara K, Furukawa T, Tsujikawa K.
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Journal Title
Scientific Reports
Volume: 9
Issue: 1
Pages: 6956-6956
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[Journal Article] Expression of Bone Morphogenetic Protein-7 Significantly Correlates With Non-small Cell Lung Cancer Progression and Prognosis:A Retrospective Cohort Study2019
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Masaya Aoki, Tadashi Umehara,Go Kamimura,Nobuhiro Imamura,Shoichiro Morizono,Yuto Nonaka,Takuya Tokunaga,Aya Harada Takeda,Koki Maeda,Yui Watanabe,Toshiyuki Nagata,Tsunayuki Otsuka,Naoya Yokomakura,Kota Kariatsumari,Masakazu Yanagi and Masami Sato
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Journal Title
Clinical Medicine Insights:Oncology
Volume: 13
Pages: 1-6
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[Journal Article] Computed tomographu-guided thoracoscopic debridement fou multiple loculated organizing empyema:a case report2019
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Masaya Aoki, Tadashi Umehara,Shoichiro Morizono,Yasuhiro Tokuda,Go Kamimura,Takuya Tokunaga,Souichi Suzuki,Aya Harada Takeda,Toshiyuki Nagata,Naoya Yokomamura,Kota Kariatumari,Kazuhiro Ueda and Masami Sato
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Journal Title
Surgical Case Reports
Volume: 174
Pages: 1-6
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[Journal Article] Successful treatment of bronchial obstruction after lobectomy in a patient with scoliosis2019
Author(s)
Masaya Aoki,MD,PhD, Kazuhiro Ueda,MD,PhD,Koki Maeda,MD,PhD,Shoichiro Morizono,MD,Tadashi Umehara,MD,Kota Kariatsumari,MD,Yasuhiro Tokuda,MD,GO Kamimura,MD,Aya Harada Takeda,MD,Toshiyuki Nagata,MD,Masami Sato,MD,PhD
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Journal Title
The Annals of Thoracic Surgery
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隈元朋洋, 冨岡勇也,松山洋美,鵜木泰自,谷川健悟,堂嶽洋一,三山秀夫,籾 博晃,佐藤雅美,井上博雅
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The Journal of the Japan Society for Respiratory Endoscopy
Volume: 41
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[Journal Article] . A prospective 5-year follow-up study after limited resection for lung cancer with ground-glass opacity.2018
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Motoyasu Sagawa, Hiroyuki Oizumi,Hiroyuki Suzuki,Hidetaka Uramoto,Katsuo Usuda,Akira Sakurada,Masayuki Chida,Satishi Shiono,Jiro Abe,Tohru Hasumi,Masami Sato,Nobuyuki Sato,Jotaro Shibuya,Hiroyuki Deguchi and Yoshinori Okada
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Journal Title
European Journal of Cardio-Thoracic Surgery
Volume: 4
Pages: 849-856
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[Journal Article] Detection of alternative subpleural lymph flow pathways using indocyanine green fluorescence2018
Author(s)
Aya Harada Takeda, Yui Watanabe,Toshiyuki Nagata,Masaya Aoki,Tadashi Umehara,Soichi Suzuki,Go Kamimura,Kazuhiro wakida,Tsunayuki Otsuka,Naoya Yokomakura,Kota Kariatsumari,Koichi Sakasegawa,Yoshihiro Nakamura,Masami Sato
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Journal Title
Surgery Today
Volume: 印刷中
Issue: 6
Pages: 640-648
DOI
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[Journal Article] Risk assessments for broncho-pleural fistula and respiratory failure after lung cancer surgery by National Clinical Database Japan.2018
Author(s)
Shunsuke Endo, Norihiro Ikeda,Takashi Kondo,Jun Nakajima,Haruhiko Kondo,Yoshihisa Shimada,Masami Sato,Shinichi Toyooka,Yoshinori Okada,Yukio Sato,Ichiro Yoshino,Morihito Okada,Meinoshin Okumura,Masayuki Chida,Eriko Fukuchi,Hiroaki Miyata
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Journal Title
General Thoracic and Cardiovascular Surgery
Volume: 3
Issue: 3
Pages: 297-305
DOI
NAID
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[Journal Article] MiR-301a/b function as oncomiRs in non-small-cell lung cancer.2018
Author(s)
Takayuki Hirono, Kentaro Jingushi,Kaori Kitae,Toshiyuki Nagata,Masami Sato,Kentaro Minami,Masaya Aoki,Aya Harada Takeda,Tadashi Umehara,Hiroshi Egawa,Yoshino Nakatsuji,Yuko Ueda,Tatsuhiko Furukawa and Kazutake Tsujikawa.
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Journal Title
Integrative Molecular Medicine.
Volume: 5
Issue: 6
Pages: 1-6
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[Journal Article] アンケートによる検診胸部X線写真読影体制の全国実態調査2018
Author(s)
三友英紀, 中山富雄,芦澤和人,遠藤千顕,小林 健,佐藤雅美,澁谷 潔,祖父江友孝,竹中大祐,西井研治,原田眞雄,前田寿美子,丸山雄一郎,三浦弘之,村田喜代史,佐川元保
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Journal Title
Japanese Journal of Lung Cancer
Volume: 4
Pages: 243-251
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[Journal Article] 肺がん検診委員会報告-肺がん検診セミナー終了にあたって-2018
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佐川元保, 中山富雄,芦澤和人,遠藤千顕,小林 健,佐藤雅美,澁谷 潔,祖父江友孝,竹中大祐,西井研二,原田眞雄,前田寿美子,丸山雄一郎,三浦弘之,三友英紀,村田喜代史
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Journal Title
apanese Journal of Lung Cancer
Volume: 2
Pages: 73-76
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[Journal Article] Ex vivo model of non-small cell lung cancer using mouse lung epithelial cells.2017
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Sato T, Morita M, Tanaka R, Inoue Y, Nomura M, Sakamoto Y, Miura K, Ito S, Sato I, Tanaka N, Abe J, Takahashi S, Kawai M, Sato M, Hippo Y, Shima H, Okada Y, Tanuma N
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Journal Title
Oncol Lett.
Volume: 14(6)
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