| Project/Area Number |
17K10848
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
栗田 浩樹 埼玉医科大学, 医学部, 教授 (70262003)
林 健 埼玉医科大学, 医学部, 教授 (40314679)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 脳血管攣縮 / くも膜下出血 / microRNA |
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| Outline of Final Research Achievements |
The aim of this study was to explore the role of microRNA(miR) in the development of cerebral vasospasm following subarachnoid hemorrhage (SAH). Peripheral blood and cerebro-spinal fluid (CSF) samples were collected from SAH patients, and microarray analysis and PCR analysis were performed. miR-15a expression was significantly increased in both the CSF and plasma, with a peak around 3 to 5 days after SAH, whereas the expression of Kruppel-like factor 4 (KLF4) was significantly decreased around 1 to 3 days after SAH and remained lower than in controls. Our results suggest that an early and persistent decrease in KLF4 followed by an increase in miR-15a may contribute to the altered vascular phenotype, resulting in development of cerebral vasospasm.
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| Academic Significance and Societal Importance of the Research Achievements |
近年、血管恒常性維持における様々なmicroRNA(miR)の関与が示唆されているが、脳血管攣縮発症におけるその役割や機能に関しては、ほとんど研究されていない。本研究では、血管新生、血管増殖に関与する可能性があるmiR15aの発現と、miR15aを介して抗増殖、抗血管新生作用を有すると考えられるKLF4の有意な発現変化を明らかにし、これにより、miRによる遺伝子発現制御機構がSAH後の脳血管収縮性変化の獲得に関与している可能性が示唆された。分泌型miRNAが血液や髄液中で安定しして存在していることから、今後、臨床上有用な生物マーカーとなる可能性を秘めている。
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