Clinical Sequence Platform for Brain Tumor

• Project/Area Number: 17K10855
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Keio University
• Principal Investigator: NISHIHARA Hiroshi 慶應義塾大学, 医学部(信濃町), 教授 (50322805)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 脳腫瘍 / クリニカルシーケンス / ゲノム解析 / 個別化診断 / がんゲノム医療 / 次世代シーケンサー / 分子病理 / 遺伝子パネル検査 / スクリーニング検査 / 遺伝子異常 / 個別化治療

Outline of Final Research Achievements

We performed 3 themes of research. 1) Sequence quality parameter : DIN value over 3.0 is the critical cut off for successful clinical sequence. 2) CN detection algorithm: We have developed original pipeline which enable us to detect 1p19q codeletion in glioma using even amplicon target sequence. 3) Clinical sequence pipeline integrated clinical information: We have ranched original clinical sequence system "PleSSision" which are already installed in more than 25 hospitals in Japan.
We have achieved initial goals in almost 100 %.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果により、脳腫瘍を病理診断時に同時にゲノム解析を行うシステムを完成させた。また、このPleSSisionシステムは、脳腫瘍のみならず、他の悪性腫瘍のゲノム診断にも応用可能である。これは、これからの統合病理遺伝子診断システム開発において、大きな一歩となる。このことにより、悪性腫瘍の質的診断の向上と、個別化治療の均てん化に大きく貢献することが可能となる。

Research Products

• [Journal Article] Intratumoral Genomic Heterogeneity May Hinder Precision Medicine Strategies in Patients with Serous Ovarian Carcinoma (2020)
  • Author(s): Nakamura Kohei、Aimono Eriko、Tanishima Shigeki、Imai Mitsuho、Nagatsuma Akiko Kawano、Hayashi Hideyuki、Yoshimura Yuki、Nakayama Kentaro、Kyo Satoru、Nishihara Hiroshi
  • Journal Title: Diagnostics Volume: 10 Issue: 4 Pages: 200-200
  • DOI: 10.3390/diagnostics10040200
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cancer gene profiling explores the possible precision medicine for diffuse-type gastric adenocarcinoma (2019)
  • Author(s): Ishikawa M, Hayashi H, Sakamoto N, Tanaka S, Nishihara H
  • Journal Title: Med Oncol. Volume: 37(1) Issue: 1 Pages: 10-10
  • DOI: 10.1007/s12032-019-1327-4
  • Peer Reviewed
• [Journal Article] Practical procedures for the integrated diagnosis of astrocytic and oligodendroglial tumors (2019)
  • Author(s): Sonoda Yukihiko、The committee for molecular diagnosis of the Japan Society of Brain Tumor Pathology、Yokoo Hideaki、Tanaka Shinya、Kinoshita Manabu、Nakada Mitsutoshi、Nishihara Hiroshi
  • Journal Title: Brain Tumor Pathology Volume: in press Issue: 2 Pages: 56-62
  • DOI: 10.1007/s10014-019-00337-y
  • Peer Reviewed
• [Presentation] “PleSSision”; a Pathologist edited Multigene Genomic Test promotes Cancer Precision Medicine. (2018)
  • Author(s): Hiroshi Nishihara
  • Organizer: The 11th International Conference of Clinical Laboratory Automation and Robotics; Cherry Blossom Symposium
  • Int'l Joint Research / Invited
• [Presentation] ゲノム診断における病理医の関与(遺伝子パネル検査における病理医の重要性) (2018)
  • Author(s): 西原 広史
  • Organizer: 第107回日本病理学会総会 ワークショップ
  • Invited
• [Presentation] ターゲットシーケンスによる神経膠腫の1p19q codel検出 (2017)
  • Author(s): 西原広史
  • Organizer: 第76回日本癌学会学術総会 腫瘍別シンポジウム
  • Invited
• [Book] 脳腫瘍 (2017)
  • Author(s): 廣瀬 隆則、小森 隆司
  • Total Pages: 240
  • Publisher: 文光堂
  • ISBN: 9784830622526