Regulatory system of RNA methylation in malignant brain tumors
| Project/Area Number |
17K10867
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Kochi University |
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Principal Investigator |
Ueba Tetsuya 高知大学, 教育研究部医療学系臨床医学部門, 教授 (00314203)
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| Co-Investigator(Kenkyū-buntansha) |
八幡 俊男 高知大学, 教育研究部医療学系臨床医学部門, 助教 (40380323)
川西 裕 高知大学, 教育研究部医療学系臨床医学部門, 助教 (90527582)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 膠芽腫 / RNA修飾 / RNAメチル化 / 幹細胞 / RNA / 核酸の修飾 / RNA methylation / Brain tumor |
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| Outline of Final Research Achievements |
Current knowledge of RNA cytosine methylation is still poor in malignant glioma. In this study, we aimed the investigation of methylation level in RNA and the search for the regulatory genes. DNA demethylase TET genes were involved with regulation of RNA methylation in glioblastoma. Alteration of RNA methylation levels were observed in glioblastoma and oligodendroglioma tissues. Several genes were identified that regulated RNA methylation and affected cell proliferation. Furthermore, it was suggested that TET3 may play a role for regulation of cell differentiation in glioblastoma. This study shows linkage of malignant brain tumor and aberration of RNA modification.
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| Academic Significance and Societal Importance of the Research Achievements |
DNAのエピジェネティックな修飾に関する研究は精力的に行われているが、RNAの修飾に関しては限られている。RNAのシトシンメチル化が転写後のRNAの寿命や翻訳効率に関わる可能性が示唆されているが、生物学的意義は十分に検討されていない。本研究において、脳腫瘍におけるRNAメチル化量の異常が検出されたことは、RNAの修飾が脳の機能に限局せず、細胞のがん形質や発がんに関与する可能性を示唆している。がん幹細胞を用いて検討することで、RNA修飾の膠芽腫における本質的な機能を見出すことが出来た。将来的には、極めて予後の悪い膠芽腫に対する新規治療に有用なRNA標的分子の同定が期待可能である。
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Attenuation of zinc-enhanced inflammatory M1 phenotype of microglia by peridinin protects against short-term spatial-memory impairment following cerebral ischemia in mice.2018
Author(s)
Ueba Y, Aratake T, Onodera KI, Higashi Y, Hamada T, Shimizu T, Shimizu S, Yawata T, Nakamura R, Akizawa T, Ueba T, Saito M.
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Journal Title
Biochem Biophys Res Commun
Volume: 507
Issue: 1-4
Pages: 476-483
DOI
Related Report
Peer Reviewed
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[Journal Article] A randomized, double-blind, phase III trial of personalized peptide vaccination for recurrent glioblastoma.2018
Author(s)
Narita Y, Arakawa Y, Yamasaki F, Nishikawa R, Aoki T, Kanamori M, Nagane M, Kumabe T, Hirose Y, Ichikawa T, Kobayashi H, Fujimaki T, Goto H, Takeshima H, Ueba T, Abe H, Tamiya T, Sonoda Y, Natsume A, Kakuma T, Sugita Y, Komatsu N, Yamada A, Sasada T, Matsueda S, Shichijo S, Itoh K, Terasaki M.
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Journal Title
Neuro Oncol.
Volume: 21
Issue: 3
Pages: 348-359
DOI
Related Report
Peer Reviewed / Open Access
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