| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
To elucidate the mechanism of interaction between regenerating axons and olfactory mucosa, we the lamina propria, with dorsal root ganglion and analyzed what factors affect neurite regrowth. Nerve growth factor（NGF） and vascular endothelial growth factor (VEGF) were released from lamina propria during the culture days. Neurites extended along the lamina propria in contact with the olfactory ensheathing cell array, which provided an efficient guide for elongation. Neurite density proceeding up to the LP significantly decreased when the lamina propria was treated with antibodies for polysialic acid-neural cell adhesion molecule (PSA-NCAM) or by removal of PSA moiety. In our co-culture experiment, treatment with neurotrophin antibodies revealed that NGF might play a role in the enhancement of neurite outgrowth. And Our results suggest that PSA bound to NCAMs might play a key role in the direct interaction between neurites and OECs.
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