New neural circuit formation in chronically injured spinal cord by neural stem cell grafts
| Project/Area Number |
17K10915
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
Kadoya Ken 北海道大学, 医学研究院, 特任准教授 (30374276)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 軸索再生 / 脊髄損傷 / 神経幹細胞 / 慢性期 / 移植 / 再生医療 / 神経科学 |
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| Outline of Final Research Achievements |
In this study, we investigated the potential of transplantation of neural stem cells at the chronic stage of spinal cord injury by comparing to sub-acute transplantation. Transplanted neural stem cells survived well in chronic lesion sites and generated many neurons. These neurons extended axons in host spinal cords in high numbers for long distances and connected to host neurons below injury. There was no significant difference of the number of extending axons between chronically and sub-acutely transplanted subjects. Further, chronically injured corticospinal tract axons regenerated into neural stem cells, though its extent was less robust than sub-acute transplantation. These findings indicate that early stage neural cells have a remarkable ability to extend axons over inhibitory environment at the chronic stage of spinal cord injury.
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| Academic Significance and Societal Importance of the Research Achievements |
ラット脊髄損傷の慢性期に、神経幹細胞を移植することで、損傷部を新しい神経組織で再構築できたことは、過去の報告から鑑みるに大きな成果だと考える。また、移植細胞が軸索を宿主内に伸展できたこと、宿主の軸索が移植内へと再生したこと、それらの軸索数が、亜急性期移植と遜色なかったことは、阻害的環境である慢性期であることを考慮すると、非常に興味深い結果と考える。これらの知見は、機能再生を達成するのが、非常に難しいとされている脊髄損傷慢性期の治療方法開発の基盤となる。
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Restorative effects of human neural stem cell grafts on the primate spinal cord.2018
Author(s)
Rosenzweig ES, Brock JH, Lu P, Kumamaru H, Salegio EA, Kadoya K, Weber JL, Liang JJ, Moseanko R, Hawbecker S, Huie JR, Havton LA, Nout-Lomas YS, Ferguson AR, Beattie MS, Bresnahan JC, Tuszynski MH.
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Journal Title
Nature Medicine
Volume: May;24(4):
Issue: 4
Pages: 484-490
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Injured adult motor and sensory axons regenerate into appropriate organotypic domains of neural progenitor grafts.2018
Author(s)
Dulin JN, Adler AF, Kumamaru H, Poplawski GHD, Lee-Kubli C, Strobl H, Gibbs D, Kadoya K, Fawcett JW, Lu P, Tuszynski MH.
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Journal Title
Nat Communications
Volume: Jan 8;9(1):84.
Issue: 1
Pages: 1-13
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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