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The study of the effect of mechanical stress on the enchondral ossification signals in the ossification of the posterior longitudinal ligament

Research Project

Project/Area Number 17K10928
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionUniversity of Fukui

Principal Investigator

Sugita Daisuke  福井大学, 学術研究院医学系部門, 助教 (90596678)

Co-Investigator(Kenkyū-buntansha) 中嶋 秀明  福井大学, 学術研究院医学系部門, 講師 (10397276)
平井 貴之  福井大学, 学術研究院医学系部門, 特別研究員 (30569132)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Keywords後縦靱帯骨化症 / インディアンヘッジホッグ / マイクロアレイ / 内軟骨骨化 / 免疫組織化学的検討 / Sox 9 / Runx 2 / メカニカルストレス / OPLL / Indian Hedgehog / 異所性骨化 / 後縦靭帯骨化症 / 頚椎 / 軟骨細胞 / 免疫組織化学的染色
Outline of Final Research Achievements

Ossification of the posterior longitudinal ligament (OPLL) is similar to enchondral ossification. Indian hedgehog (Ihh) and its related signaling are key factors in normal enchondral ossification.In the microarray analysis, Ihh and its related genes showed increased expression levels compared with controls after the 24-hour mechanical strain. In the immunoblotting analysis, Ihh, Sox9, Runx2, Gli2, Gli3, and smoothened (SMO) showed significantly increased their protein expression levels after mechanical strain. In addition, OPLL samples were strongly immunopositive for Ihh, Sox9, Runx2, and Gli3 in the ossification front of OPLL. The expression levels of Ihh and its related genes are upregulated significantly by cyclic tensile strain. These results suggest that cyclic tensile strain induces abnormal activation of Ihh and its related signaling, which might be important in the ossification process in OPLL.

Academic Significance and Societal Importance of the Research Achievements

後縦靱帯骨化症(OPLL)の骨化巣の発生や進展機序は未だ不明である。治療法としては対症的な薬物療法か、進行例では神経症状を緩和するための外科的な治療法が存在するが、骨化巣が摘出できるのは限られた一部の症例であり、また、残存した骨化巣の進展による神経症状の再増悪も起こりえる。もしもOPLLの骨化巣進展の機序が解明できれば、骨化巣の進展を制御できる内科的な治療法の開発の一端を担える可能性があると考える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (1 results)

All 2020

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results)

  • [Journal Article] Cyclic tensile strain facilitates ossification of the cervical posterior longitudinal ligament via increased Indian hedgehog signaling2020

    • Author(s)
      Sugita D, Nakajima H, Kokubo Y, Takeura N, Yayama T, Matsumine A.
    • Journal Title

      Sci Rep.

      Volume: 10(1) Issue: 1 Pages: 7231-7231

    • DOI

      10.1038/s41598-020-64304-w

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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