| Project/Area Number |
17K10956
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Uehara Kosuke 東京大学, 医学部附属病院, 助教 (20599063)
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| Co-Investigator(Kenkyū-buntansha) |
森崎 裕 東京大学, 医学部附属病院, 講師 (30508099)
矢野 文子 東京大学, 医学部附属病院, 特任准教授 (80529040)
張 成虎 東京大学, 医学部附属病院, 助教 (80780551)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 腱 / 腱再生 / LMD / RNAシークエンス / FACS / laser micro dissection / 医療・福祉 |
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| Outline of Final Research Achievements |
To identify the mechanism involved in tendon regeneration, we analyzed time-dependent changes in fibrous tissue that regenerated after total excision of the Achilles tendon of 8-week-old C57BL / 6J mice. The regenerated tissue was completely different from the normal tendon with heterotopic cartilage and calcification in which the collagen arrangement was disturbed even 3 months after the operation, and the results were the almost same in the real time PCR. Furthermore, we extracted RNAs from multiple regenerated tissues over time using the Laser micro dissection (LMD) method and performed RNA sequence, and identified multiple cytokines and molecular signals that might be involved in tendon regeneration.
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| Academic Significance and Societal Importance of the Research Achievements |
腱の障害は、若年者のスポーツ外傷から高齢者の変性断裂に至るまで、全年齢層で起こりうる病態であり日常生活に重大な影響を与えることが知られている。一方、骨や軟骨と比べると、腱の再生に関しての研究は決して盛んとは言えず、多くは謎に包まれている。本研究において得られた知見は腱再生のメカニズムの解明につながる可能性があり、この知見を用いた腱障害の新たな薬剤や治療法の開発は腱障害における重大な日常生活の影響を低減するだけでなく、医療費の軽減にもつながる事が期待される。
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