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Applied treatment for cartilage using tenascin C domain (TNIIIA2)

Research Project

Project/Area Number 17K11003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionMie University

Principal Investigator

HASEGAWA Masahiro  三重大学, 医学系研究科, 准教授 (40308664)

Co-Investigator(Kenkyū-buntansha) 須藤 啓広  三重大学, 医学系研究科, 教授 (60196904)
吉田 利通  三重大学, 医学系研究科, リサーチアソシエイト (80166959)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords関節軟骨 / 軟骨変性 / テネイシンC / サイトカイン / 再生医学 / 変形性関節症 / マトリセルラープロテイン
Outline of Final Research Achievements

TNIIIA2 is a peptide of tenascin-C. We evaluated whether TNIIIA2 could prevent articular cartilage degeneration without inducing synovitis in vivo study. TNIIIA2 was injected into the knee joint of mice to evaluate the induction of synovitis. Synovitis was not enhanced with TNIIIA2 administration. TNIIIA2 was injected into the knee joint of post-traumatic osteoarthritis mice model. Histologic examinations were made. Development of osteoarthritis was suppressed.
An in vitro study was also performed using cultured human chondrocytes. The expressions of various mRNAs were compared with TNIIIA2 treatment using real-time polymerase chain reaction. TNIIIA2 upregulated the expressions of tumor necrosis factor-α, matrix metalloproteinase 3, and basic fibroblast growth factor. In conclusion, we demonstrated that TNIIIA2 could prevent cartilage degeneration without synovitis.

Academic Significance and Societal Importance of the Research Achievements

軟骨治療は非常に困難であり、軟骨損傷、変性は変形性関節症を誘発する。変形性関節症の予防・治療に利用する新しい候補分子として、軟骨修復能、軟骨変性抑制効果を有するマトリセルラープロテインであるテネイシンC(TNC)に着目してきた。しかし、TNCは炎症を誘発する可能性があり、特定のドメインであるTNIIIA2を用いた実験を行ってきた。軟骨変性抑制効果のある薬物は臨床応用されていないが、TNIIIA2がその候補となりえることが示唆された。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (14 results)

All 2020 2019 2018 2017

All Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results) Presentation (10 results) (of which Int'l Joint Research: 5 results)

  • [Journal Article] TNIIIA2, The Peptide of Tenascin-C, as a Candidate for Preventing Articular Cartilage Degeneration2020

    • Author(s)
      Hattori Tetsuya、Hasegawa Masahiro、Unno Hironori、Iino Takahiro、Fukai Fumio、Yoshida Toshimichi、Sudo Akihiro
    • Journal Title

      CARTILAGE

      Volume: - Issue: 2_suppl Pages: 1367S-1375S

    • DOI

      10.1177/1947603520912300

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Tenascin-C promotes the repair of cartilage defects in mice.2019

    • Author(s)
      Unno H, Hasegawa M, Suzuki Y, Iino T, Imanaka-Yoshida K, Yoshida T, Sudo A.
    • Journal Title

      J Orthop Sci.

      Volume: 印刷中 Issue: 2 Pages: 324-330

    • DOI

      10.1016/j.jos.2019.03.013

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Role of tenascin-C in articular cartilage.2018

    • Author(s)
      Hasegawa M, Yoshida T, Sudo A.
    • Journal Title

      Mod Rheumatol.

      Volume: 28 Issue: 2 Pages: 215-220

    • DOI

      10.1080/14397595.2017.1349560

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Tenascin-C Prevents Articular Cartilage Degeneration in Murine Osteoarthritis Models.2018

    • Author(s)
      Matsui Y, Hasegawa M, Iino T, Imanaka-Yoshida K, Yoshida T, Sudo A.
    • Journal Title

      Cartilage.

      Volume: 9 Issue: 1 Pages: 80-88

    • DOI

      10.1177/1947603516681134

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] The peptide of Tenascin-C promotes chondrocyte proliferation by activating Integrin-FAK-PI3K/Akt pathway2020

    • Author(s)
      Yoshio Hattori, Masahiro Hasegawa, Tetsuya Hattori, Takahiro Iino, Fumio Fukai, Toshimichi Yoshida, Akihiro Sudo
    • Organizer
      Orthopaedic Research Society Annual Meeting 2020
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] TNIIIA2 as a candidate for preventing articular cartilage degeneration 2018

    • Author(s)
      Tetsuya Hattori, Masahiro Hasegawa, Hironori Unno, Shine Tone, Yohei Naito, Takahiro Iino, Fumio Fukai, Toshimichi Yoshida, Akihiro Sudo
    • Organizer
      19th European Federation of National Associations of Orthopaedics and Traumatology (EFORT) Congress
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] テネイシンCのペプチドであるTNIIIA2のマウス関節内投与による効果2018

    • Author(s)
      服部徹也、長谷川正裕、海野宏至、森川正和、細井敬、刀根慎恵、内藤陽平、須藤啓広
    • Organizer
      第33回日本整形外科学会基礎学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] TNIIIA2による軟骨変性抑制効果の検討:TNIIIA2の関節内投与による軟骨変性抑制効果の検討2018

    • Author(s)
      服部徹也、長谷川正裕、海野宏至、若林弘樹、須藤啓広
    • Organizer
      第62回日本リウマチ学会
    • Related Report
      2018 Research-status Report
  • [Presentation] テネイシンCペプチドの軟骨細胞における効果2018

    • Author(s)
      服部徹也、長谷川正裕、森川正和、吉田利通、今中恭子、須藤啓広
    • Organizer
      第5回Matricellフォーラム
    • Related Report
      2018 Research-status Report
  • [Presentation] TNIIIA2, the peptide of tenascin-C, prevents degeneration of cartilage without synovitis in mice osteoarthritis model2018

    • Author(s)
      Tetsuya Hattori, Masahiro Hasegawa, Hironori Unno, Masakazu Morikawa, Takashi Hosoi, Takahiro Iino, Fumio Fukai, Toshimichi Yoshida, Akihiro Sudo
    • Organizer
      Orthopaedic Research Society Annual Meeting 2018
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Prevention of cartilage degeneration using the peptide of tenascin-C 2017

    • Author(s)
      Tetsuya Hattori, Masahiro Hasegawa, Hironori Unno, Takashi Hosoi, Naoya Ito, Takahiro Iino, Kyoko Imanaka-Yoshida, Toshimichi Yoshida, Akihiro Sudo
    • Organizer
      18th European Federation of National Associations of Orthopaedics and Traumatology (EFORT) Congress
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Elastin is useful for ligament-bone junction healing in rabbits 2017

    • Author(s)
      Naoya Ito, Masahiro Hasegawa, Tetsuya Hattori, Takashi Hosoi, Hironori Unno, Yoshiaki Suzuki, Yoshihiro Miura, Yuriyo Matsui, Keiichi Miyamoto, Akihiro Sudo
    • Organizer
      18th European Federation of National Associations of Orthopaedics and Traumatology (EFORT) Congress
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] テネイシンCを用いた関節軟骨の修復・変性抑制2017

    • Author(s)
      長谷川正裕, 服部徹也, 伊東直也, 細井 敬, 今中(吉田)恭子, 吉田利通, 須藤啓広
    • Organizer
      第49回日本結合組織学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] TNIIIA2による軟骨変性抑制効果の検討2017

    • Author(s)
      服部 徹也, 長谷川 正裕, 海野 宏至, 細井 敬, 伊東 直也, 刀根 慎恵, 内藤 陽平, 山口 敏郎, 若林 弘樹, 須藤 啓広
    • Organizer
      第32回日本整形外科学会基礎学術集会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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