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The analysis of plastic change in spinal dorsal horn after peripheral nerve injury using spinal cord slice with attached both side of dorsal roots.

Research Project

Project/Area Number 17K11104
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Anesthesiology
Research InstitutionNiigata University

Principal Investigator

Hiroshi Baba  新潟大学, 医歯学系, 教授 (00262436)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords脊髄スライス / 末梢神経損傷 / 細胞内Ca / 光学的測定 / 両側後根刺激 / 成熟ラット / 末梢神経障害 / 細胞内Caイメージング / 神経障害性疼痛 / Ca2+イメージング / 神経障害痛モデル / 後根 / イメージング / 膜電位 / 細胞内カルシウム / 成熟動物 / 脊髄後角 / Caイメージング / 両側後根
Outline of Final Research Achievements

Neuropathic pain after peripheral nerve injury is thought to be caused by the augmented activity of spinal cord dorsal horn (DH). However, whether peripheral nerve injury augments the activity of DH neurons evoked by peripheral tissue stimulation is controversial. To address this issue, we undertook intracellular Ca2+ imaging studies using spinal cord slices with attached both side dorsal roots, and examined if the excitability of injured side of dorsal horn is more increased than normal side. The excitability of injured side is not increased, but actually decreased. It is unlikely that peripheral nerve injury augments the activity of DH neurons.

Academic Significance and Societal Importance of the Research Achievements

臨床で遭遇する神経障害性疼痛患者の痛みの原因は脊髄における可塑性変化の結果、末梢から伝わってきた感覚信号が脊髄で増幅されて上位中枢である脳に伝わるためと考えられてきた。しかし、今回の実験結果から、脊髄から脳に送られる信号はむしろ弱まることが示唆され、脳への信号が弱まっても、それを痛みとして感じるのは脳における何らかの可塑性変化のためと予想された。
本研究の結果は、今後の神経障害性疼痛の発生機序の解明は脳の感覚野あるいは辺縁系(情動系)をターゲットとすべきことを示唆している。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2019

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (1 results)

  • [Journal Article] Neurosteroid dehydroepiandrosterone sulphate enhances pain transmission in rat spinal dorsal horn2019

    • Author(s)
      Yamamoto Goh、Kamiya Yoshinori、Sasaki Mika、Ikoma Miho、Baba Hiroshi、Kohno Tatsuro
    • Journal Title

      British Journal of Anaesthesia

      Volume: 印刷中 Issue: 2 Pages: e215-e225

    • DOI

      10.1016/j.bja.2019.03.026

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mechanisms of noradrenergic modulation of synaptic transmission and neuronal excitability in ventral horn neurons of the rat spinal cord2019

    • Author(s)
      Shoji H, Ohashi M, Hirano T, Watanabe K, Endo N, Baba H, Kohno T
    • Journal Title

      Neuroscience

      Volume: 408 Pages: 161-176

    • DOI

      10.1016/j.neuroscience.2019.03.026

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acute spatial spread of NO-mediated potentiation during hindpaw ischaemia in mice.2019

    • Author(s)
      Onishi T, Watanabe T, Sasaki M, Kamiya Y, Horie M, Tsukano H, Hishida R, Kohno T, Takebayashi H, Baba H, Shibuki K
    • Journal Title

      J Physiol.

      Volume: 597 Issue: 13 Pages: 3441-3455

    • DOI

      10.1113/jp277615

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Action of Norepinephrine on Lamina X of the Spinal Cord2019

    • Author(s)
      Ohashi N, Ohashi M, Baba H
    • Journal Title

      Neuroscience

      Volume: 408 Pages: 214-225

    • DOI

      10.1016/j.neuroscience.2019.04.004

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Optimal Position of Inferior Vena Cava Cannula in Pediatric Cardiac Surgery: A Prospective, Randomized, Controlled, Double-Blind Study2019

    • Author(s)
      Seino Y, Ohashi N, Imai H, Baba H
    • Journal Title

      J Neurosurg Anesthesiol

      Volume: 33 Issue: 5 Pages: 1253-1259

    • DOI

      10.1053/j.jvca.2018.10.023

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 臨床濃度のメサドンは脊髄後角浅層部細胞の興奮性を強く抑制するが、NMDA受容体チャネルには作用しない - 細胞内Ca2+高速イメージング法とホールセルパッチクランプ法を用いた検討 -2019

    • Author(s)
      馬場 洋
    • Organizer
      第66回日本麻酔科学会
    • Related Report
      2018 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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