| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
TRPV4 and Cx43 channels are involved in the control of bladder contractile response. Blockade of each of them attenuates bladder overactivity in cyclophosphamide (CYP)-induced cystitis. It is unclear why two different channels similarly regulate bladder activity. We speculated that there existed a close interaction between TRPV4 and Cx43 channels under pathological situations, which contributed to the initiation and development of bladder abnormality. The aim of this study was to test this hypothesis.In a mouse model of CYP cystitis, TRPV4 and Cx43 proteins were found to be markedly increased. Further analysis revealed that the interaction between TRPV4 and Cx43 channels contributed to the regulation of the intracellular redox status and inflammasome activation. Collectively, our study indicates that the interaction between TRPV4 and Cx43 underlies the bladder abnormality through mechanisms involving its actions on the intracellular oxidative status and inflammatory response.
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