| Project/Area Number |
17K11193
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | University of Occupational and Environmental Health, Japan |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
松本 正広 産業医科大学, 医学部, 助教 (20580294)
|
|---|
| Project Period (FY) |
2017-04-01 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
|
|---|
| Keywords | mycoplasma genitalium / 尿道炎 / マクロライド耐性 / キノロン耐性 / moxifloxacin 耐性 / parC / TMA / Mycoplasma genitalium / Serine / efflux pump / moxifloxacin耐性 / sitafloxacin耐性 / アミノ酸変異 / 多剤耐性 / テトラサイクリン耐性 |
|---|
| Outline of Final Research Achievements |
From Japanese patients with non-gonococcal urethritis, new 13 Mycoplasma genitalium strains were isolated and cultured. We analyzed antimicrobial susceptibility and genome mutations of total 18 strains including 13 new isolates, type strain and 4 strains isolated at 2014. Among these strains, 12 strains were macrolide-resistant and had MRAM on 23S rRNA gene. In addition, 9 strains had MIC with more than 1 mg/ml against moxifloxacin (MFLX. By sequence of QRDR of ParC, strains with more than 1 mg/L MIC of MFLX had mutation with amino-acid change such as Ser83→Ile, but strains with mutation such as Ser83→Asn did not elevate MIC of MFLX.To know the sensitivity of transcription-mediated amplification (TMA) method, the detection limit was measured among 20 stocked M. genitalium strains. The detection limit of TMA method were 0,03-0.87 DNA copies.
|
| Academic Significance and Societal Importance of the Research Achievements |
Mycoplasma genitaliumは男性尿道炎、子宮頚管炎の原因微生物である。近年、薬剤耐性が進行しており、クラミジア感染症と同じ抗菌薬で治療困難例が増加している。本研究では、M. genitaliumの分離培養法を行い、新しい株の薬剤耐性、遺伝子変異の研究を行った。13株を分離し、うち12株がマクロライド耐性、９株がキノロン耐性であった。キノロン耐性はParCのキノロン耐性決定領域のシークエンスを行い、83番のSerineがisoleucineeへのアミノ酸変異を含む遺伝子変異が牽連があることを見出した。
|
