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Role of prostaglandin D2 in the differentiation of ovarian granulosa cells

Research Project

Project/Area Number 17K11214
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionAsahikawa Medical College

Principal Investigator

Imamichi Yoshitaka  旭川医科大学, 医学部, 助教 (00570194)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsprostagladin D2 / ovary / プロスタグランジンD2 / 卵巣顆粒膜細胞 / プロスタグランジン / PGD2 / 卵巣 / 顆粒膜細胞
Outline of Final Research Achievements

Among prostanoids including the prostaglandins (PGs) and the thromboxanes, PGE2 and PGF2α are the pivotal lipid mediators for normal ovarian functions. We found that the expression of the lipocalin-type PGD synthase (L-PGDS) was up-regulated in ovarian granulosa cells by the overexpression of the transcription factor steroidogenic factor-1, which is essential for ovarian development and function. In this study, we attempted to clarify the roles of PGD2 in ovarian granulosa cells. The expression of L-PGDS was increased in the luteinized mouse ovary. We also found that the expression of an aldo-keto reductase (AKR1CL) which catalyzes the conversion of prostaglandins was increased in the luteinized mouse ovary. These results suggested that the ovarian PGD2 exerts functions in luteal phase via conversion by AKR1CL.

Academic Significance and Societal Importance of the Research Achievements

プロスタグランジン(PG)E2およびPGF2αは、ステロイドホルモン合成、排卵、卵丘細胞の膨潤や黄体退縮などに関与することが解明されてきたが、卵巣におけるPGD2の役割はそのほとんどが不明であった。我々は、マウス卵巣の黄体化に伴い、PGD2合成・代謝に関与するL-PGDSとAKR1CLの遺伝子発現が亢進すること、さらにL-PGDSとAKR1CLの両者が卵巣形成に必須の転写因子SF-1に制御されることを見出した。本研究結果は、黄体の形成、維持および退縮の機序解明につながり、得られた知見は黄体機能不全の原因究明と治療法の開発の一助となることが期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (18 results)

All 2020 2019 2018 2017

All Journal Article (7 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (11 results)

  • [Journal Article] Evaluation of 17β-hydroxysteroid dehydrogenase activity using androgen receptor-mediated transactivation2020

    • Author(s)
      Yazawa Takashi、Imamichi Yoshitaka、Uwada Junsuke、Sekiguchi Toshio、Mikami Daisuke、Kitano Takeshi、Ida Takanori、Sato Takahiro、Nemoto Takahiro、Nagata Sayaka、Islam Khan Md. Rafiqul、Takahashi Satoru、Ushikubi Fumitaka、Suzuki Nobuo、Umezawa Akihiro、Taniguchi Takanobu
    • Journal Title

      The Journal of Steroid Biochemistry and Molecular Biology

      Volume: 196 Pages: 105493-105493

    • DOI

      10.1016/j.jsbmb.2019.105493

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcriptional Regulation of Ovarian Steroidogenic Genes; Recent Findings Obtained from Stem Cell-derived Steroidogenic Cells.2019

    • Author(s)
      Yazawa, T., Imamichi, Y., Sekiguchi, T., Miyamoto, K., Uwada, J., Khan, R., Suzuki, N., Umezawa A. and Taniguchi, T.
    • Journal Title

      BioMed Research International

      Volume: 8973076 Pages: 1-13

    • DOI

      10.1155/2019/8973076

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cyclooxygenase‐2 is acutely induced by CCAAT/enhancer‐binding protein β to produce prostaglandin E 2 and F 2α following gonadotropin stimulation in Leydig cells2019

    • Author(s)
      Yazawa Takashi、Imamichi Yoshitaka、Yuhki Koh‐ichi、Uwada Junsuke、Mikami Daisuke、Shimada Masayuki、Miyamoto Kaoru、Kitano Takeshi、Takahashi Satoru、Sekiguchi Toshio、Suzuki Nobuo、Rafiqul Islam Khan Md.、Ushikubi Fumitaka、Umezawa Akihiro、Taniguchi Takanobu
    • Journal Title

      Molecular Reproduction and Development

      Volume: 86 Issue: 7 Pages: 786-797

    • DOI

      10.1002/mrd.23163

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Prostaglandin F2α Facilitates Platelet Activation by Acting on Prostaglandin E2 Receptor Subtype EP3 and Thromboxane A2 Receptor TP in Mice2019

    • Author(s)
      Kashiwagi Hitoshi、Yuhki Koh-ichi、Imamichi Yoshitaka、Kojima Fumiaki、Kumei Shima、Tasaki Yoshikazu、Narumiya Shuh、Ushikubi Fumitaka
    • Journal Title

      Thrombosis and Haemostasis

      Volume: 119 Issue: 08 Pages: 1311-1320

    • DOI

      10.1055/s-0039-1688906

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ステロイドホルモンと性スペクトラム2019

    • Author(s)
      矢澤隆志、北野健、今道力敬
    • Journal Title

      実験医学

      Volume: 37 Pages: 1442-1446

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Roles of prostanoids in the regulation of platelet function.2018

    • Author(s)
      Kashiwagi H, Yuhki K, Imamichi Y, Kojima F, Kumei S, Narumiya S, Ushikubi F
    • Journal Title

      Thrombosis & Haemostasis: Research

      Volume: 2 Pages: 1-4

    • Related Report
      2018 Research-status Report
    • Open Access
  • [Journal Article] Diethylstilbestrol administration inhibits theca cell androgen and granulosa cell estrogen production in immature rat ovary2017

    • Author(s)
      Imamichi, Y., Sekiguchi, T., Kitano, T., Kajitani, T., Okada, R., Inaoka, Y., Miyamoto, K., Uwada, J., Takahashi, S., Nemoto, T., Mano, A., Khan, M.R.I., Islam, M.T., Yuhki, K., Kashiwagi, H., Ushikubi, F., Suzuki, N., Taniguchi, T., Yazawa, T.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 8374-8374

    • DOI

      10.1038/s41598-017-08780-7

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 第 4 回ペプチド・ホルモン研究会シンポジウム~多様な動物種を用いたホルモン研究の新展開~2019

    • Author(s)
      今道力敬、矢澤隆志
    • Organizer
      第90 回 日本動物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 精巣・ライディッヒ細胞における COX-2 の転写調節機構2019

    • Author(s)
      矢澤隆志, 今道力敬
    • Organizer
      日本動物学会 北海道支部 第63回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] DESによる幼若ラット卵巣のステロイドホルモン産生の抑制2018

    • Author(s)
      矢澤隆志、今道力敬、宇和田淳介、谷口隆信
    • Organizer
      第89回 日本動物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 第3回ペプチド・ホルモン研究会シンポジウム ~多様な動物種における普遍および特異な内分泌現象の解明~2018

    • Author(s)
      今道力敬、矢澤隆志
    • Organizer
      日本動物学会 第89回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] DES投与が幼若ラット卵巣のステロイドホルモン合成に及ぼす影響の解析2018

    • Author(s)
      矢澤隆志, 今道力敬, 宮本薫, 宇和田淳介, 谷口隆信
    • Organizer
      弟91回 日本内分泌学会学術総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Analysis of steroidogenic pathway using stem cell-derived steroidogenic cells2018

    • Author(s)
      Yazawa Takashi, Imamichi Yoshitaka, Uwada Junsuke, Taniguchi Takanobu
    • Organizer
      弟91回 日本生化学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Diethylstilbesterolは卵巣ステロイド合成を抑制する2017

    • Author(s)
      今道力敬
    • Organizer
      第8回ペプチド・ホルモン研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] 哺乳類における11-ケトテストステロン産生2017

    • Author(s)
      矢澤 隆志,今道 力敬, 宇和田 淳介,谷口 隆信
    • Organizer
      日本動物学会 第88回富山大会
    • Related Report
      2017 Research-status Report
  • [Presentation] DES投与が幼若ラット卵巣のステロイドホルモン産生に及ぼす影響2017

    • Author(s)
      矢澤隆志、今道力敬
    • Organizer
      日本動物学会北海道支部第62回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 幹細胞からのステロイドホルモン産生細胞の分化誘導2017

    • Author(s)
      矢澤 隆志, 今道 力敬, 宮本 薫, 谷口 隆信
    • Organizer
      第90回日本内分泌学会学術総会
    • Related Report
      2017 Research-status Report
  • [Presentation] 11-ケトテストステロンはエストロゲンへと変換されないアンドロゲンとして機能する2017

    • Author(s)
      今道 力敬, 結城 幸一, 折坂 誠, 北野 健, 向井 邦晃, 牛首 文隆, 谷口 隆信, 梅澤 明弘, 宮本 薫, 矢澤 隆志
    • Organizer
      第90回日本内分泌学会学術総会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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