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Mechanism of Glycine Receptor Agonist Effect on Embryonic Development and Application to the Prevention of Oocyte Aging

Research Project

Project/Area Number 17K11222
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionUniversity of Toyama

Principal Investigator

Nishizono Hirofumi  富山大学, 学術研究部医学系, 助教 (10502289)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords卵子老化 / グリシンレセプター / 初期発生 / 生殖医学 / アミノ酸受容体
Outline of Final Research Achievements

In this study, we found that the glycine receptor α4 subunit was expressed in mouse fertilized eggs. Also, the inhibition of the glycine receptor α4 subunit function by inhibitors or gene disruption caused embryonic development to stop after the 4-cell stage. Furthermore, we have shown that enhancing glycine receptor function improves the developmental rate of low-developmental-rate fertilized eggs. Thus, we have shown that the glycine receptor α4 subunit is essential for early development.

Academic Significance and Societal Importance of the Research Achievements

本研究はこれまで哺乳類では機能が明らかになっていなかったグリシンレセプターα4がマウスの受精卵で発現しており、さらに4細胞期以降の発生に重要であることを示したこと、またその機能亢進により受精率の向上効果が期待できることを示した点で重要である。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2020 2019 2018 2017

All Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Glycine receptor α4 subunit facilitates the early embryonic development in mice2020

    • Author(s)
      Nishizono Hirofumi、Darwish Mohamed、Endo Takaho A、Uno Kyosuke、Abe Hiroyuki、Yasuda Ryohei
    • Journal Title

      Reproduction

      Volume: 159 Issue: 1 Pages: 41-41

    • DOI

      10.1530/rep-19-0312

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice2020

    • Author(s)
      Nishizono Hirofumi、Darwish Mohamed、Uosaki Hideki、Masuyama Nanami、Seki Motoaki、Abe Hiroyuki、Yachie Nozomu、Yasuda Ryohei
    • Journal Title

      Journal of Visualized Experiments

      Volume: - Issue: 158 Pages: 1-1

    • DOI

      10.3791/60808

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Rapid and high-efficient generation of mutant mice using freeze-thawed embryos of the C57BL/6J strain2019

    • Author(s)
      Darwish Mohamed、Nishizono Hirofumi、Uosaki Hideki、Sawada Hitomi、Sadahiro Taketaro、Ieda Masaki、Takao Keizo
    • Journal Title

      Journal of Neuroscience Methods

      Volume: 317 Pages: 149-156

    • DOI

      10.1016/j.jneumeth.2019.01.010

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cleavage Speed and Blastomere Number in DBA/2J Compared with C57BL/6J Mouse Embryos2017

    • Author(s)
      Nishizono H, Uno K, Abe H.
    • Journal Title

      Journal of the American Association for Laboratory Animal Science

      Volume: 56 Pages: 11-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Glycine Receptor Α4 Subunit Supports The Early Development Of Mouse Embryos.2019

    • Author(s)
      西園啓文
    • Organizer
      52nd SSR Annual Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Generation of glycine receptor alpha 4 knockout mice using high-efficient modified CRISPR-Cas9 protocol.2018

    • Author(s)
      Mohamed Darwish
    • Organizer
      Neuroscience 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Generation and Characterization of Glycine Receptor Alpha 4 Knockout Mice.2018

    • Author(s)
      西園啓文
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 分子探索支援により明らかになったグリシンレセプターα4サブユニットの卵子および脳での機能2018

    • Author(s)
      西園啓文
    • Organizer
      先端モデル動物支援プラットフォーム・若手支援技術講習会
    • Related Report
      2018 Research-status Report
  • [Presentation] Generation of Glycine Receptor Alpha 4 knockout Mice Using High-efficient Modified CRISPR-Cas9 Protocol.2018

    • Author(s)
      Mohamed Darwish
    • Organizer
      先端モデル動物支援プラットフォーム成果発表会
    • Related Report
      2018 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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