Investigation of ceRNA regulatory networks in trophoblast cells and application of ceRNA in prediction of preeclampsia
| Project/Area Number |
17K11256
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nippon Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大口 昭英 自治医科大学, 医学部, 教授 (10306136)
竹下 俊行 日本医科大学, 大学院医学研究科, 大学院教授 (60188175)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 産科学 / 妊娠 / 胎盤 / ceRNA / ncRNA / 妊娠高血圧腎症 / 競合内在性RNA |
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| Outline of Final Research Achievements |
Non-coding RNAs (ncRNAs) may have a potential role in extravillous trophoblast (EVT) invasion in the human placenta during early pregnancy. We investigated the gene expression profile of EVT cells by RNA-seq and evaluated a role of ncRNAs in EVT invasion. We found that small ncRNA miR-675-5p, which is derived from long ncRNA H19, was highly expressed in EVT cells and accelerated EVT cell invasion. Furthermore, we revealed that GATA2, a transcriptional factor gene, was a direct target mRNA of miR-675-5p and that miR-675-5p-mediated GATA2 suppression accelerated EVT invasion through matrix metalloproteinase-dependent signaling pathway.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、従来にないceRNA［リボ核酸（RNA）、特に、蛋白質をコードしていないRNA（ncRNA）と蛋白質をコードしているRNA（mRNA）同士が相互作用（競合）して遺伝子発現を調節するとこが示唆されており、これら相互作用するRNAを競合内在性RNA（ceRNA）と呼ぶ］による側面から、トロホブラスト（栄養膜細胞）における遺伝子発現制御ネットワークの解明、およびceRNAの妊娠高血圧腎症（PE）の分子病態への関与に関する先駆け研究となった。
ceRNAに焦点を当てたPE予知因子探索・同定は早期予知に結びつく可能性が高く、この研究の継続発展は、周産期医療への貢献が期待できる。
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Report
(4 results)
Research Products
(51 results)
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[Journal Article] Temporary hypertension and white coat hypertension in the first trimester as risk factors for preeclampsia2019
Author(s)
Ohkuchi A, Hirashima C, Arai R, Takahashi K, Suzuki H, Ogoyama M, Nagayama S, Takahashi H, Baba Y, Usui R, Shirasuna K, Matsubara S
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Journal Title
Hypertens Res
Volume: 42
Issue: 12
Pages: 2002-2012
DOI
Related Report
Peer Reviewed
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[Journal Article] Outline of the new definition and classification of “Hypertensive Disorders of Pregnancy (HDP)”; a revised JSSHP statement of 20052018
Author(s)
Watanabe K, Matsubara K, Nakamoto O, Ushijima J, Ohkuchi A, Koide K, Makino S, Mimura K, Morikawa M, Naruse K, Tanaka K, Nohira T, Metoki H, Kawabata I, Takeda S, Seki H, Takagi K, Yamasaki M, Ichihara A, Kimura T, Saito S
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Journal Title
Hypertension Research in Pregnancy
Volume: 6
Issue: 2
Pages: 33-37
DOI
NAID
ISSN
2187-5987, 2187-9931
Year and Date
2018-11-30
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[Presentation] 妊娠高血圧腎症,HELLP症候群の発症週数別sFlt-1,PlGF,及びsFlt-1/PlGFの比較2018
Author(s)
鈴木寛正, 平嶋周子, 永山志穂, 小古山学, 馬場洋介, 高橋宏典, 高橋佳代, 薄井里英, 大口昭英, 松原茂樹
Organizer
第70回日本産科婦人科学会学術講演会
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[Presentation] Change in microRNA expression in preeclamptic maternal blood : Sixty such microRNA detected by microarray analysis (3D-Gene chip) with global normalization2017
Author(s)
Hironori Takahashi, Manabu Ogoyama, Shiho Nagayama, Yoichi Ishida, Hirotada Suzuki, Yosuke Baba, Tomoyuki Kuwata, Rie Usui, Toshihiro Takizawa, Akihide Ohkuchi, Shigeki Matsubara
Organizer
第69回日本産科婦人科学会学術講演会
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