Molecular origin of chromosomal abnormalities in early embryonic cells
Project/Area Number |
17K11259
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Obstetrics and gynecology
|
Research Institution | Fujita Health University |
Principal Investigator |
Kato Takema 藤田医科大学, 総合医科学研究所, 助教 (20387690)
|
Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | PGT / 染色体異常 / モザイク / 着床前診断 / 受精卵 / PGT-A / PGT-SR / NGS / 胚盤胞 / Blastocyst / Inner Cell Mass / Trophectoderm / Chromosome / PGD / PGS / ICM / TE / 初期胚 |
Outline of Final Research Achievements |
In this study, we performed chromosome copy number analysis of arrested embryos and blastocysts to examine the details of chromosomal abnormalities that occur during fertilization and how the chromosomal abnormalities affect the mitotic cell division during early embryonic stage. Chromosome analysis of arrested embryos revealed that almost all of them had chromosomal abnormalities and also had mosaic chromosomal abnormalities in multiple chromosomes. Chromosome comparative analysis between ICMs, which form the fetus, and TEs, which form the placenta, showed that karyotypes differed in about half of the embryos. The cause of the discrepancy between ICMs and TEs was found to be a mosaic chromosomal abnormalities that occurred post-fertilization embryos and tended to accumulate in TEs. Furthermore, the karyotype concordance rate among TEs was only about 59.1%. These results indicate that the chromosomal karyotype of TEs in PGT cannot is unlikely to reflect the ICM or the embryo.
|
Academic Significance and Societal Importance of the Research Achievements |
本研究による成果として、受精卵の個々の細胞を解析したことから、初期胚における染色体異常を持つ細胞が、胚盤胞期にICMやTEにどのように分化し、アポトーシスを受け核型不一致を起こすかヒントになり得る結果を得た。さらに上記の基礎研究の成果のみならず、TE生検によって得られた細胞を鋳型にして、PGT-AやPGT-SRのように染色体解析した場合、どのような染色体異常を持つとICMとTE間で不一致になりやすいかなど診断精度の指標を作成することができた。本研究の成果は、基礎研究のみならず臨床としても有意義のある成果と考えている。
|
Report
(4 results)
Research Products
(39 results)
-
[Journal Article] A case of a parthenogenetic 46,XX/46,XY chimera presenting ambiguous genitalia.2020
Author(s)
Kawamura R, Kato T, Miyai S, Suzuki F, Naru Y, Kato M, Tanaka K, Nagasaka M, Tsutsumi M, Inagaki H, Ioroi T, Yoshida M, Nao T, Conlin LK, Iijima K, Kurahashi H, Taniguchi-Ikeda M.
-
Journal Title
J Hum Genet.
Volume: -
Issue: 8
Pages: 705-709
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Preimplantation genetic testing for aneuploidy: a comparison of live birth rates in patients with recurrent pregnancy loss due to embryonic aneuploidy or recurrent implantation failure.2019
Author(s)
Sato T, Sugiura-Ogasawara M, Ozawa F, Yamamoto T, Kato T, Kurahashi H, Kuroda T, Aoyama N, Kato K, Kobayashi R, Fukuda A, Utsunomiya T, Kuwahara A, Saito H, Takeshita T, Irahara M.
-
Journal Title
Hum Reprod.
Volume: 34
Issue: 12
Pages: 2340-2348
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Exome-First Approach in Fetal Akinesia Reveals Chromosome 1p36 Deletion Syndrome.2019
Author(s)
Toshimitsu M, Nagaoka S, Kobori S, Ogawa M, Suzuki F, Kato T, Miyai S, Kawamura R, Inagaki H, Kurahashi H, Murotsuki J.
-
Journal Title
Case Rep Obstet Gynecol.
Volume: -
Pages: 1-5
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
[Journal Article] DNA methylation accumulation in gastric mucosa adjacent to cancer after Helicobacter pylori eradication.2019
Author(s)
Tahara S, Tahara T, Horiguchi N, Kato T, Shinkai Y, Yamashita H, Yamada H, Kawamura T, Terada T, Okubo M, Nagasaka M, Nakagawa Y, Shibata T, Yamada S, Urano M, Tsukamoto T, Kurahashi H, Kuroda M, Ohmiya N.
-
Journal Title
Int J Cancer.
Volume: 144
Issue: 1
Pages: 80-88
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Prenatal diagnosis of premature chromatid separation/mosaic variegated aneuploidy (PCS/MVA) syndrome.2018
Author(s)
Yamaguchi T, Yamaguchi M, Akeno K, Fujisaki M, Sumiyoshi K, Ohashi M, Sameshima H, Ozaki M, Kato M, Kato T, Hosoba E, Kurahashi H.
-
Journal Title
J Obstet Gynaecol Res.
Volume: 44
Issue: 7
Pages: 1313-1317
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Potential role for nectin-4 in the pathogenesis of pre-eclampsia: a molecular genetic study.2018
Author(s)
Ito M, Nishizawa H, Tsutsumi M, Kato A, Sakabe Y, Noda Y, Ohwaki A, Miyazaki J, Kato T, Shiogama K, Sekiya T, Kurahashi H, Fujii T.
-
Journal Title
BMC Med Genet.
Volume: 19
Issue: 1
Pages: 166-166
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] A Japanese case of β-ureidopropionase deficiency with dysmorphic features2017
Author(s)
Akiyama, T., Shibata, T., Yoshinaga, H., Kuhara, T., Nakajima, Y., Kato, T., Maeda, Y., Ohse, M., Oka, M., Kageyama, M., Kobayashi, K.
-
Journal Title
Brain and Development
Volume: 39
Issue: 1
Pages: 58-61
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] How does DNA break for formation of three-way translocations?2018
Author(s)
Takema Kato, Hidehito Inagaki, Yasuko Shinkai, Asuka Kato, Rie Kawamura, Makiko Tsutsumi, Mariko Taniguchi-Ikeda, Seiji Mizuno, Yoshio Makita, Nakamichi Saito, Nobuhiko Ochi, Hiroki Kurahashi
Organizer
The American Society of Human Genetics
Related Report
Int'l Joint Research
-
-
[Presentation] Comprehensive chromosomal analysis of blastomeres with developmental arrest2017
Author(s)
Takema Kato, Sachie Nishiyama, Yukio Nishyama, Kaori Yoshikai, Yukino Matsuda, Tomio Sawada, Hikari Yoshizawa, Hiroshi Furukawa, Haruki Nishizawa, Maki Kato, Asuka Kanbayashi, Hiroki Kurahashi
Organizer
International Conference on Prenatal Diagnosis and Therapy
Related Report
Int'l Joint Research
-
-
-
-