| Project/Area Number |
17K11274
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kyoto University |
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Principal Investigator |
Tani Hirohiko 京都大学, 医学研究科, 助教 (70615252)
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 幸保 京都大学, 医学研究科, 非常勤講師 (00508236)
伊藤 美幸 京都大学, 医学研究科, 特定病院助教 (00760951)
堀江 昭史 京都大学, 医学研究科, 講師 (30535836)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 腹膜因子 / 子宮内膜症 / in vivo / エストロゲン非依存性 / 持続分泌 / 生着期 / マイクロアレイ / ダグラス窩 / 恒久的 / 発現上昇 / 子宮内膜症形成モデル / サンプル収集 / 解析 / 子宮内膜自家腹膜移植 / versicanノックアウトマウス / ステロイドホルモン / 腹膜 |
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| Outline of Final Research Achievements |
To investigate possible roles of peritoneal versican in the development of endometriosis using mouse model.Mouse model of developing endometriosis was prepared by intraperitoneally administration of endometrial tissue collected from donor mice to C57BL/6 8- to 9-week-old recipient mice. V1-CM or control-CM was intraperitoneallyinjected to model mice for continuous 5 days. Intraperitoneal endometriotic lesions were macroscopically confirmed. Experiments were approved by the Committee of the Institute for Animal Experimentation of Kyoto University Hospital. Results: Endometriotic lesions were observed in the peritoneal cavity of all mice. The total volume of the endometriotic lesion was significantly larger in V1-CM group than in the control group Conclusions: Our results suggest that peritoneal versican is involved in the developmentof peritoneal endometriosis.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究期間において腹膜因子であるversicanの子宮内膜症形成への関与をin vivoでの検証結果が得られ、versicanの発現がエストロゲン非依存性であることが示された。これらにより腹膜因子が子宮内膜症形成に関与することがより強く示唆され、一連の検証モデルが他の腹膜因子検証に用いることができる確証が得られた。
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