| Project/Area Number |
17K11379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 頭頸部外科学 / 蛍光イメージング / 酸化ストレス |
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| Outline of Final Research Achievements |
We applied gGlu-HMRG, a probe for detecting GGT activity, to head and neck carcinoma resected specimens, and fluorescence was detected in about half of the specimens. Comprehensive RNA sequencing and pathway analysis of the head and neck cancer resected specimens showed that GGT1, a major subtype of GGT, was highly expressed in about half of the specimens. In the high GGT1 group, FGF21 was highly expressed and PI3K pathway was activated. In addition, GGT1 was highly expressed in Detroit562 cells derived from head and neck cancers, where the PI3K pathway is activated, suggesting a relationship between GGT1 activity and the PI3K pathway.
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| Academic Significance and Societal Importance of the Research Achievements |
当初の目的である頭頸部癌における酸化ストレス耐性機構の解明にまでは至らなかったが、細胞内GSH濃度と関連のあるGGT活性につき、蛍光イメージングの結果からGGT活性とPI3K経路の関連という新たな知見が得られたことは、蛍光イメージングによって同じ頭頸部癌における違う特徴が検出可能であることを示す重要な成果と考えられる。
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