| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
In this present study, we successfully established a modified cell expansion method that increases the efficiency of expanding an undifferentiated human corneal endothelial cell (CEC) subpopulation to approximately 1.5 times higher than the previously used method by suppressing the stratification of the cells and the changes to a fibroblast-like morphology. Our novel method successfully expands an undifferentiated human CEC subpopulation by approximately 10,000 times over a 5-week period. Moreover, we found that pretreatment with specific functional low-molecular-weight compounds used for the induction of the differentiation of pluripotent differentiated CECs increases the efficiency of those cells to differentiate to mature CECs. These findings allowed us to successfully establish a cell differentiation method that induces an undifferentiated human CEC subpopulation to mature CECs at an efficiency of approximately 85%.
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