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The role of microRNA in immune regulation and neuro-protection of autoimmune uveoretinitis

Research Project

Project/Area Number 17K11489
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ophthalmology
Research InstitutionKyorin University

Principal Investigator

Watanabe Takayo  杏林大学, 医学部, 非常勤講師 (90458901)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords眼炎症 / ぶどう膜炎
Outline of Final Research Achievements

microRNAs (miRNAs) are small, noncoding, and regulatory RNAs and regulate gene expression through repression of the expression of target genes. Our previous study revealed that the expression of miRNA -223, 142, and 146 in retina was elevated, whereas the expression of miRNA-181, 183, 124, and 331 was decreased during development of experimental autoimmune uveoretitnitis (EAU). The present study demonstrated that intravitreal injection of miRNA-124 significantly reduced the pathological socre of EAU in rats. Furthermore, we investigated the changes of miRNA expression in serum during the development of EAU. Microarray analysis demonstrated that the expression of miRNA-3544 and 146a was increased, whereas the expression of miRNA-100 and 130a was reduced on day 14 after immunization. These findings were confirmed by quantitative PCR method. These results suggest that the up-regulated or down-regulated miRNA in serum s may play regulatory roles for development and regulation of EAU.

Academic Significance and Societal Importance of the Research Achievements

以前に我々はEAUの眼局所におけるmiRNAの発現について報告したが、今回の研究から、immune-regulatory miRNAとして知られるmiRNA-146が眼局所に加えて全身的にもEAUの炎症抑制に関与している可能性が示唆された。本研究課題におけるmiRNAを介したEAUに対する炎症制御・神経保護作用の解析、および血液中のmiRNAの網羅的発現解析を通じて難治性ぶどう膜炎に対する新規治療標的分子の同定、miRNAを用いた新たな疾患活動性バイオマーカーの確立へと発展していくことが期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (14 results)

All 2020 2019 2018 2017

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (11 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Clinical features, treatment, and visual outcomes of Japanese patients with posterior scleritis2020

    • Author(s)
      Ando Y, Keino H, Nakayama M, Watanabe T, Okada AA
    • Journal Title

      Ocul Immunol Inflamm

      Volume: 28 Issue: 2 Pages: 209-216

    • DOI

      10.1080/09273948.2019.1574838

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinical features of patients with diabetic anterior uveitis.2019

    • Author(s)
      Watanabe T, Keino H, Nakayama K, Taki W, Echizen N, Okada AA.
    • Journal Title

      Br J Ophthalmol.

      Volume: 103 Issue: 1 Pages: 78-82

    • DOI

      10.1136/bjophthalmol-2017-311453

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinical features and visual outcomes of 111 patients with new-onset acute Vogt-Koyanagi-Harada disease treated with pulse intravenous corticosteroids.2019

    • Author(s)
      Nakayama M, Keino H, Watanabe T, Okada AA.
    • Journal Title

      Br J Ophthalmol.

      Volume: 103 Issue: 2 Pages: 274-278

    • DOI

      10.1136/bjophthalmol-2017-311691

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] Decreased optic nerve head blood flow in acute retinal necrosis2019

    • Author(s)
      Keino H, Nakayama M, Watanabe T, Ando Y, Koto T, Hirota K, Inoue M, Okada AA
    • Organizer
      Association for Research in Vision and Ophthalmology (ARVO)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 当院における眼サルコイドーシス60例の臨床経過の検討2019

    • Author(s)
      長堀克哉, 中山真紀子, 慶野博, 安藤良将, 渡辺交世, 岡田アナベルあやめ
    • Organizer
      第53回日本眼炎症学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 原田病および交感性眼炎に対するアダリムマブ導入例の検討2019

    • Author(s)
      中山真紀子, 慶野博, 渡辺交世, 安藤良将, 林勇海, 岡田アナベルあやめ
    • Organizer
      第73日本臨床眼科学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Long term efficacy of infliximab in refractory uveoretinitis associated with Behcet’s disease2019

    • Author(s)
      Keino H, Nakayama M, Watanabe T, Ando Y, Abe S, Hayashi H, Fukuoka K, Komagata Y, Okada AA
    • Organizer
      Internatioonal Ocular Inflammation Society (IOIS) 2019 Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of the use of adalimumab in the treatment of VKH disease and sympathetic ophthalmia2019

    • Author(s)
      Nakayama M, Abe S, Hayashi I, Keino H, Watanabe T, Ando Y, Okada AA
    • Organizer
      Internatioonal Ocular Inflammation Society (IOIS) 2019 Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CMV網膜炎を発症したGood症候群の1例.2018

    • Author(s)
      津田麻祐子、慶野博、中山真紀子、倉井大輔、高橋あずさ、渡辺交世、岡田アナベルあやめ
    • Organizer
      第122回日本眼科学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] 超広角眼底撮影による原田病急性期の眼底造影所見の検討.2018

    • Author(s)
      中山真紀子、慶野博、安藤良将、渡辺交世、岡田アナベルあやめ
    • Organizer
      第72回日本臨床眼科学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Compromised optic nerve head blood flow in cytomegalovirus retinitis.2018

    • Author(s)
      Mayuko Tsuda, Hiroshi Keino, Makiko Nakayama, Takayo Watanabe, and Annabelle A Okada
    • Organizer
      Association for Research in Vision and Ophthalmology (ARVO)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 超広角眼底撮影による原田病急性期の眼底造影所見の検討2017

    • Author(s)
      中山真紀子,慶野博,安藤良将、渡辺交世,岡田アナベルあやめ
    • Organizer
      第51回日本眼炎症学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 杏林アイセンターにおける後部強膜炎の臨床像の検討2017

    • Author(s)
      安藤良将、慶野博, 中山真紀子,安藤良将、渡辺交世,岡田アナベルあやめ
    • Organizer
      第51回日本眼炎症学会
    • Related Report
      2017 Research-status Report
  • [Presentation] サイトメガロウイルス網膜炎における視神経乳頭部血流の評価2017

    • Author(s)
      中山真紀子、慶野博、高橋あずさ、津田麻祐子、渡辺交世、岡田アナベルあやめ
    • Organizer
      第71回日本臨床眼科学会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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