Elucidation of the mechanism of hypoxic response of adipose stem cells and development of a new in vitro amplification method
| Project/Area Number |
17K11558
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
楠本 健司 関西医科大学, 医学部, 教授 (20161630)
森本 尚樹 関西医科大学, 医学部, 准教授 (40378641)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 脂肪幹細胞 / 低酸素 / 増殖 / FGF-2 / HIF / Src / 血管新生 / 再生医療 |
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| Outline of Final Research Achievements |
ASCs were prepared from normal adipose tissue of human lower abdomen, and the cell proliferation of ASCs was promoted by hypoxia, and the increase of HIF-1α was observed under hypoxia due to their activation. The knockdown of HIF-1α decreased the mRNA of FGF-2, indicating that the HIF-1α binding site of the FGF-2 gene is involved in the proliferation of ASCs. Therefore, we elucidated that the mechanism of proliferation promotion of adipose stem cells under hypoxia involves the action of FGF-2 through the expression of HIF-1α. We also found that Src is involved in the FGF-2-mediated proliferative effect of ASCs.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究から、 低酸素下における脂肪幹細胞の増殖促進機構が明らかになった。低酸素下の脂肪幹細胞は増殖が促進し、FGF-2 や VEGF などの増殖因子も多量に分泌することが明らかになった。これらの培養法や増殖因子が分泌された培養上清は、今後の再生医療応用への利用に役立つものと考えられる。
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Report
(5 results)
Research Products
(7 results)
