| Project/Area Number |
17K11579
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Emergency medicine
|
|---|
| Research Institution | Sapporo Medical University |
|---|
Principal Investigator |
Uemura Shuji 札幌医科大学, 医学部, 講師 (10448607)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
横田 伸一 札幌医科大学, 医学部, 教授 (10325863)
文屋 尚史 札幌医科大学, 医学部, 助教 (50721586)
白石 宗 札幌医科大学, 医学部, 助教 (70725168)
|
|---|
| Project Period (FY) |
2017-04-01 – 2023-03-31
|
| Project Status |
Completed (Fiscal Year 2022)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | fatty acid / antibacterial activity / Pseudomonas aeruginosa / MRSA / colistin / 遊離脂肪酸 / 抗菌活性 / 熱傷 / 救急蘇生学 |
|---|
| Outline of Final Research Achievements |
Palmitoleic acid and myristoleic acid showed antibacterial activity against Staphylococcus aureus alone, and aminoglycoside-resistant MRSA was inhibited by the combination use of aminoglycosides. No fatty acids had antibacterial activity against Pseudomonas aeruginosa, and there was almost no effect in combination with aminoglycosides. Colistin showed an antimicrobial effect with Cis-type unsaturated fatty acids and an antagonistic effect with all fatty acids. This study revealed fatty acids with antimicrobial activity against Staphylococcus aureus, but not Pseudomonas aeruginosa, which is a problem in burns, as expected. Cis-unsaturated fatty acids were found to antagonize colistin, suggesting that antagonism of free fatty acids should be considered when topical colistin is used.
|
| Academic Significance and Societal Importance of the Research Achievements |
脂肪酸は人的無害な物質であり、脂肪酸による抗菌活性は薬剤耐性化が問題となる熱傷創感染の大きな解決策になる可能性を秘めていた。今回の研究で熱傷創感染の原因菌であるMRSAには抗菌作用が十分にあることが示されたが緑膿菌への抗菌活性はなかった。さらに薬剤耐性緑膿菌の切り札であるコリスチンに関しては一部の脂肪酸が拮抗作用を持つことがわかった。我々が今回調べた脂肪酸では熱傷創治療へ大きな期待はできないことがわかった。
|
