Types of alveolar epithlial cell death during ARDS

• Project/Area Number: 17K11582
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Saitama Children's Medical Center (2019); Yokohama City University (2017-2018)
• Principal Investigator: Uchimoto Kazuhiro 埼玉県立小児医療センター (臨床研究部), 集中治療科, 医長 (50710951)
• Co-Investigator(Kenkyū-buntansha): 水原 敬洋 横浜市立大学, 附属病院, 講師 (00637712) ; 東條 健太郎 横浜市立大学, 医学部, 講師 (80737552)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ARDS / 人工呼吸 / 細胞死 / ネクローシス / アポトーシス / 臓器不全

Outline of Final Research Achievements

The aim of this proect was investigating the relative contribution of necrosis and apoptosis for the alveolar epithelial cell death in ARDS patients, utilizing CK18-M30 and M65 as markers. We have confirmed the validity of these markers using animal models. However, we could not investigate about the types of alveolar epithelial cell death in human ARDS patients.

Academic Significance and Societal Importance of the Research Achievements

本研究によってARDSにおいてBALF中のCK18-M30，およびM65が肺胞上皮細胞死のタイプ特異的なマーカーになりうることが示唆された．しかしながら，実際のARDS患者での検証には至らず，課題が残る結果となった．

Research Products

• [Journal Article] Necrosis Rather Than Apoptosis is the Dominant form of Alveolar Epithelial Cell Death in Lipopolysaccharide-Induced Experimental Acute Respiratory Distress Syndrome Model (2020)
  • Author(s): Tamada Nao、Tojo Kentaro、Yazawa Takuya、Goto Takahisa
  • Journal Title: Shock Volume: 54 Issue: 1 Pages: 128-139
  • DOI: 10.1097/shk.0000000000001425
  • Peer Reviewed
• [Presentation] 高容量換気はARDSにおける肺胞上皮細胞ネクローシスを増強する (2020)
  • Author(s): 鶴雄斗，玉田尚，東條健太郎，後藤隆久
  • Organizer: 第47回日本集中治療医学会学術集会
• [Presentation] ARDSにおける肺胞上皮細胞死はアポトーシスorネクローシス？-LPS誘導性ARDSモデルを用いた検討- (2019)
  • Author(s): 玉田尚，東條健太郎，後藤隆久
  • Organizer: 第46回日本集中治療医学会学術集会
• [Presentation] Necrosis rather than apoptosis is the dominant type of alveolar epithelial cell death in LPS-induced experimental ARDS (2019)
  • Author(s): Tamada Nao, Tojo Kentaro, Goto Takahisa
  • Organizer: 39th International Symposium on Intensive Care and Emergency Medicine
  • Int'l Joint Research
• [Presentation] ARDS における肺胞上皮細胞死はアポトーシス or ネクロー シス?:LPS 誘導性 ARDS モデルを用いた検討 (2018)
  • Author(s): 玉田尚，東條健太郎，後藤隆久
  • Organizer: 第４６回日本集中治療医学会学術集会
• [Presentation] Necrosis, rather than apoptosis, is the dominant type of alveolar epithelial cell death in LPS-induced experimental ARDS (2018)
  • Author(s): Nao Tamada, Kentaro Tojo, Takahisa Goto
  • Organizer: 39th International Symposium on Intensive Care and Emergency Medicine
  • Int'l Joint Research