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Elucidation of the regulatory mechanisms for bacterial virulence with small RNAs.

Research Project

Project/Area Number 17K11627
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionShowa University

Principal Investigator

Morisaki Hirobumi  昭和大学, 歯学部, 講師 (30317581)

Co-Investigator(Kenkyū-buntansha) 深町 はるか  昭和大学, 歯学部, 助教 (10433799)
桑田 啓貴  昭和大学, 歯学部, 教授 (60380523)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsStreptococcus sanguinis / csRNA / Streptococcus oralis / 細菌 / 核酸 / 感染症
Outline of Final Research Achievements

We targeted Streptococcus sanguinis, which is the main causative agent of infective endocarditis, and analyzed the regulatory mechanism of pathogenicity of the bacteria, focusing on small RNA (sRNA). We constructed a strain lacking csRNA, which is a type of sRNA of S. sanguinis, to examine the function of csRNA, and it became clear that the expression of pilT, which is one of the genes constituting the type IV pili operon, is suppressed by csRNA. Furthermore, the biofilm-forming ability was increased in the csRNA-deficient strain. These results suggest that the csRNA of S. sanguinis may regulate pilT in response to the changes in the external environment and regulate the expression of pathogenicity of the bacteria.

Academic Significance and Societal Importance of the Research Achievements

近年、様々な薬剤耐性菌の増加が報告されており、新たな感染症治療法の開発は重要な課題である。しかし、従来の病原菌の殺滅を目的とするような方法では耐性菌の出現は避けられず、新たな視点に立った治療法の開発が必要である。本研究は細菌の排除ではなく病原性の発現を抑制する方法の開発を目指し、細菌の病原性発現機構の解析を行った。口腔常在菌の一種で、血流中では病原性を発揮するStreptococcus sanguinisにおける解析の結果、低分子RNAが病原性の発現に重要な働きをすることが明らかとなった。この研究成果は低分子RNAを標的とした新たな感染症治療法の開発の基盤となり、きわめて意義深いと考えられる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Streptococcus sanguinis non-coding csRNAs negatively regulate expression of type IV pilus retraction ATPase PilT and biofilm formation2018

    • Author(s)
      Ota C, Morisaki H, Nakata M, Arimoto T, Fukamachi H, Kataoka H, Masuda Y, Suzuki N, Miyazaki T, Okahashi N, Kuwata H
    • Journal Title

      Infect Immun

      Volume: 86 Issue: 3 Pages: 1-13

    • DOI

      10.1128/iai.00894-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Streptococcus sanguinisのcsRNAによるⅣ型線毛pilT遺伝子の発現制御2019

    • Author(s)
      森崎 弘史
    • Organizer
      第51回レンサ球菌研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] Streptococcus sanguinisのノンコーディングcsRNAはⅣ型線毛pilT遺伝子の発現を負に制御し、バイオフィルム形成を抑制する2017

    • Author(s)
      大田 千明、森崎 弘史、有本 隆文、深町 はるか、片岡 嗣雄、増田 宜子、鈴木 規元、宮﨑 隆、桑田 啓貴
    • Organizer
      第59回歯科基礎医学会学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Regulation of type IV pilus retraction ATPase pilT by small noncoding RNAs in Streptococcus sanguinis2017

    • Author(s)
      Chiaki Ota, Hirobumi Morisaki, Takafumi Arimoto, Haruka Fukamachi, Hideo Kataoka, Yoshiko Masuda, Noriyuki Suzuki, Takashi Miyazaki,Hirotaka Kuwata
    • Organizer
      第65回国際歯科研究学会日本部会総会・学術大会
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2022-01-27  

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