| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
The purpose of this study was to elucidate the mechanism that commensal bacteria including oral bacteria and pathogenic bacteria are involved in the maintenance of intestinal homeostasis via interaction with Paneth cells. In this study, a novel ex vivo evaluation system to visualize and quantify Paneth cell granule secretion was established by microinjection method of bacteria into the lumen of enteroids, three-dimensional cultures of small intestinal epithelial cells. Paneth cells secreted granules immediately when live non-commensal bacterium S. Typhimurium or commensal bacterium B. bifidum was introduced into the enteroid lumen by microinjection, whereas Paneth cells did not secrete granules when live commensal bacterium L. casei was introduced.
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