Elucidation of homeostatic mechanism via intestinal environment regulated by oral bacteria
| Project/Area Number |
17K11661
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 自然免疫 / Paneth細胞 / αディフェンシン / 免疫 / 感染 / 炎症 |
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| Outline of Final Research Achievements |
The purpose of this study was to elucidate the mechanism that commensal bacteria including oral bacteria and pathogenic bacteria are involved in the maintenance of intestinal homeostasis via interaction with Paneth cells. In this study, a novel ex vivo evaluation system to visualize and quantify Paneth cell granule secretion was established by microinjection method of bacteria into the lumen of enteroids, three-dimensional cultures of small intestinal epithelial cells. Paneth cells secreted granules immediately when live non-commensal bacterium S. Typhimurium or commensal bacterium B. bifidum was introduced into the enteroid lumen by microinjection, whereas Paneth cells did not secrete granules when live commensal bacterium L. casei was introduced.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により樹立した共生細菌および病原菌に応答したPaneth細胞顆粒分泌評価システムは、Paneth細胞 αディフェンシンを中心とする新たな腸内細菌叢制御を介した生体恒常性維持のメカニズム解明を可能とする。この評価系により、口腔内を含む、様々な生体に共生する菌と腸管上皮細胞との相互作用を細胞・分子レベルで解析することにより、共生細菌と腸管のクロストークを介した恒常性の維持機構が明らかとなり、共生細菌と宿主のバランスの破綻が原因の一つと考えられている、生活習慣病などに対する新たな予防・治療戦略の提案が期待できる。
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Analysis of Serotonin in Human Feces Using Solid Phase Extraction and Column-Switching LC-MS/MS2020
Author(s)
Hirabayashi Y, Nakamura K, Sonehara T, Suzuki D, Hanzawa S, Shimizu Y, Aizawa T, Nakamura K, Tamakoshi A, Ayabe T
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Journal Title
Mass Spectrometry
Volume: 9
Issue: 1
Pages: A0081-A0081
DOI
NAID
ISSN
2186-5116, 2187-137X
Year and Date
2020-03-31
Related Report
Peer Reviewed / Open Access
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[Journal Article] Associations of gut microbiota, dietary intake, and serum short-chain fatty acids with fecal short-chain fatty acids2020
Author(s)
Yamamura R, Nakamura K, Kitada N, Aizawa T, Shimizu Y, Nakamura K, Ayabe T, Kimura T, Tamakoshi A
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Journal Title
Bioscience of Microbiota, Food and Health
Volume: 39
Issue: 1
Pages: 11-17
DOI
NAID
ISSN
2186-3342
Related Report
Peer Reviewed / Open Access
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[Journal Article] Myc-induced nuclear antigen constrains a latent intestinal epithelial cell-intrinsic anthelmintic pathway.2019
Author(s)
Pillai MR, Mihi B, Ishiwata K, Nakamura K, Sakuragi N, Finkelstein DB, McGargill MA, Nakayama T, Ayabe T, Coleman ML, Bix M
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Journal Title
PLoS One
Volume: 26;14(2):e0211244
Issue: 2
Pages: 1-21
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] R-Spondin1 expands Paneth cells and prevents dysbiosis induced by graft-versus-host disease2017
Author(s)
Hayase E, Hashimoto D, Nakamura K, Noizat C, Ogasawara R, Takahashi S, Ohigashi H, Yokoi Y, Sugimoto R, Matsuoka S, Ara T, Yokoyama E, Yamakawa T, Ebata K, Kondo T, Hiramine R, Aizawa T, Ogura Y, Hayashi T, Mori H, Kurokawa K, Tomizuka K, Ayabe T, Teshima T
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Journal Title
J Exp Med
Volume: 214
Issue: 12
Pages: 3507-3518
DOI
NAID
Related Report
Peer Reviewed
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