The mechanism of periodontal tissue destruction by proteases from periodontal bacteria Porpyromonas gingivalis.
| Project/Area Number |
17K11668
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Pathobiological dentistry/Dental radiology
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
大森 一弘 岡山大学, 大学病院, 講師 (20549860)
後藤 和義 岡山大学, 医歯薬学域, 助教 (20626593)
大原 直也 岡山大学, 医歯薬学域, 教授 (70223930)
|
|---|
| Project Period (FY) |
2017-04-01 – 2022-03-31
|
| Project Status |
Completed (Fiscal Year 2021)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 歯周病菌 / 歯周炎 / ジンジパイン / 炎症応答 / 細胞内シグナル伝達 / 歯周病細菌 / COX-2 / 細胞内カルシウム / 歯周病 / プロスタグランジン / シクロオキシゲナーゼ-2 / 炎症 / Porphyromonas gingivalis / 歯周組織の炎症 / 細胞応答 / 歯周病原細菌 |
|---|
| Outline of Final Research Achievements |
The purpose of this study is to reveal the molecular mechanism of infection between periodontal pathogenic bacteria and host cells using cellular and molecular biological approaches, and to analyze the function of pathogenic proteases produced by periodontal pathogenic bacteria to clarify the pathogenesis of periodontal disease. In this study, we focused on gingipains, which are cysteine proteases, produced by Porphyromonas gingivalis (P. gingivalis), to elucidate the pathogenesis of periodontal disease infected with P. gingivalis. We examined the host cell response to the molecular mechanism of COX-2 expression by gingipains and showed that activation of two signaling pathways, ERK and IKK, and found that intracellular calcium is required for COX-2 expression and PGE2 production as the upstream factors.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究は歯周病細菌が産生するプロテアーゼが炎症を誘導する因子であることを示す研究である。それを明らかにできれば、プロテアーゼを産生する口腔細菌が歯周病に関与する重要な細菌であることがわかり、プロテアーゼ産生細菌を増やさないようにすることが歯周病予防に努める指標となると思われる。
|
Report
(6 results)
Research Products
(37 results)
-
[Journal Article] The fungal metabolite (+)-terrein abrogates ovariectomy-induced bone loss and receptor activator of nuclear factor-κB ligand-induced osteoclastogenesis by suppressing protein kinase-C α/βII phosphorylation.2021
Author(s)
Kyosuke Sakaida, Kazuhiro Omori, Masaaki Nakayama, Hiroki Mandai, Saki Nakagawa, Hidefumi Sako, Chiaki Kamei, Satoshi Yamamoto, Hiroya Kobayashi, Satoki Ishii, Mitsuaki Ono, Soichiro Ibaragi, Keisuke Yamashiro, Tadashi Yamamoto, Seiji Suga and Shogo Takashiba
-
Journal Title
Frontiers in Pharmacology
Volume: 12
Pages: 674366-674366
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Attempt of thyX gene silencing and construction of a thyX deleted clone in a Mycobacterium bovis BCG.2021
Author(s)
Yuki Arimura, Yusuke Minato, Takayuki Wada, Masaaki Nakayama, Ayako Ryumon, Nao Hirata, Chie Nakajima, Yasuhiko Suzuki, Manabu Ato, Kazuo Kobayashi, Naoko Ohara, Seiji Iida, Naoya Ohara
-
Journal Title
Microbiol Immunol
Volume: 66
Issue: 1
Pages: 10-14
DOI
Related Report
Peer Reviewed
-
[Journal Article] Comparative Study of the Susceptibility to Oxidative Stress between Two Types of Mycobacterium bovis BCG Tokyo 1722021
Author(s)
Keiichi Taniguchi, Daisuke Hayashi, Naomi Yasuda, Mao Nakayama, Kaori Yazawa, Shouta Ogawa, Yuji Miyatake, Saki Suda, Haruka Tomita, Miki Tokuda, Saotomo Itoh, Jun-Ichi Maeyama, Naoya Ohara, Saburo Yamamoto, Shigeaki Hida, Kikuo Onozaki and Takemasa Takii
-
Journal Title
mSphere
Volume: 6
Issue: 2
Pages: 1-11
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] The Fungal Metabolite (+)-Terrein Abrogates Osteoclast Differentiation via Suppression of the RANKL Signaling Pathway through NFATc12020
Author(s)
S. Nakagawa, K. Omori, M. Nakayama, H. Mandai, S. Yamamoto, H. Kobayashi, H. Sako, K. Sakaida, H. Yoshimura, S. Ishii, S. Ibaragi, K. Hirai, K. Yamashiro, T. Yamamoto, S. Suga
-
Journal Title
Int. Immunopharmacol
Volume: 83
Pages: 106429-106429
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] Fungal metabolite (+)-terrein suppresses IL-6/sIL-6R-induced CSF1 secretion by inhibiting JAK1 phosphorylation in human gingival fibroblasts2018
Author(s)
Satoshi Yamamoto, Kazuhiro Omori, Hiroki Mandai, Masaaki Nakayama, Saki Nakagawa, Hiroya Kobayashi, Tadashi Kunimine, Hiroshi Yoshimura, Kyosuke Sakaida, Hidefumi Sako, Soichiro Ibaragi, Tadashi Yamamoto, Hiroshi Maeda, Seiji Suga, and Shogo Takashiba
-
Journal Title
Heliyon
Volume: 4
Issue: 11
Pages: e00979-e00979
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
