| Project/Area Number |
17K11668
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
大森 一弘 岡山大学, 大学病院, 講師 (20549860)
後藤 和義 岡山大学, 医歯薬学域, 助教 (20626593)
大原 直也 岡山大学, 医歯薬学域, 教授 (70223930)
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| Project Period (FY) |
2017-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 歯周病菌 / 歯周炎 / ジンジパイン / 炎症応答 / 細胞内シグナル伝達 / 歯周病細菌 / COX-2 / 細胞内カルシウム / 歯周病 / プロスタグランジン / シクロオキシゲナーゼ-2 / 炎症 / Porphyromonas gingivalis / 歯周組織の炎症 / 細胞応答 / 歯周病原細菌 |
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| Outline of Final Research Achievements |
The purpose of this study is to reveal the molecular mechanism of infection between periodontal pathogenic bacteria and host cells using cellular and molecular biological approaches, and to analyze the function of pathogenic proteases produced by periodontal pathogenic bacteria to clarify the pathogenesis of periodontal disease. In this study, we focused on gingipains, which are cysteine proteases, produced by Porphyromonas gingivalis (P. gingivalis), to elucidate the pathogenesis of periodontal disease infected with P. gingivalis. We examined the host cell response to the molecular mechanism of COX-2 expression by gingipains and showed that activation of two signaling pathways, ERK and IKK, and found that intracellular calcium is required for COX-2 expression and PGE2 production as the upstream factors.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は歯周病細菌が産生するプロテアーゼが炎症を誘導する因子であることを示す研究である。それを明らかにできれば、プロテアーゼを産生する口腔細菌が歯周病に関与する重要な細菌であることがわかり、プロテアーゼ産生細菌を増やさないようにすることが歯周病予防に努める指標となると思われる。
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