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Estimation of the sub-cellular diffusion using multi-exponential fitting model

Research Project

Project/Area Number 17K11674
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionNagasaki University

Principal Investigator

SASAKI Miho  長崎大学, 病院(歯学系), 助教 (10437874)

Co-Investigator(Kenkyū-buntansha) 佛坂 由可  長崎大学, 病院(歯学系), 講師 (10244089)
中村 卓  長崎大学, 医歯薬学総合研究科(歯学系), 客員研究員 (30172406)
高木 幸則  長崎大学, 医歯薬学総合研究科(歯学系), 助教 (30295084)
片山 郁夫  長崎大学, 医歯薬学総合研究科(歯学系), 助教 (80295089)
榮田 智  長崎大学, 医歯薬学総合研究科(歯学系), 助教 (80325662)
角 美佐  長崎大学, 医歯薬学総合研究科(歯学系), 教授 (90284702)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords拡散強調MRI / 細胞内拡散 / 制限拡散 / 拡散強調撮像 / MRI
Outline of Final Research Achievements

The aim of this study was to establish the theoretical model for the assessment of restricted diffusion using extremely high b-value diffusion-weighted MRI and reveal the sub-cellular water diffusivity distinguishing from extracellular water. We performed diffusion-weighted MRI of the cell block and cellular-components (nucleus, plasma membrane, organelle membrane, chromatin and DNA) and calculated the diffusion coefficients using multi-exponential curve fitting algorithms. As a result, sub-cellular diffusions were divided into three compartments, suggesting the diffusivities of cytosol, plasma/organelle membranes and intra-nuclear structures. Therefore, we have shown the potential of diffusion-weighted MR imaging with extremely high b-values for estimating subcellular water diffusivity, which could provide the useful information for the various biological process in the cell, including intracellular transport, protein-DNA/RNA interactions, cell division and cell death processes.

Academic Significance and Societal Importance of the Research Achievements

本研究を臨床応用し、生体組織や各種疾患・病変の細胞内の水分子の拡散を細胞外のそれと区別して評価し、その特徴や病変ごとの違いなどを明らかにする事ができれば、拡散現象を通して細胞増殖や細胞死、さらには癌化などといった多様な生命現象に伴う核や細胞内小器官の変化を2次元/3次元的に捉える事が可能で、更に病期の診断や治療効果判定に有用なデータを非侵襲的に提供できると考えられ、学術的・社会的意義は大きい。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2021 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results)

  • [Journal Article] Combined classification system based on ACR/EULAR and ultrasonographic scores for improving the diagnosis of Sjogren's syndrome2018

    • Author(s)
      Takagi Y, Nakamura H, Sumi M, Shimizu T, Horai Y, Eida S, Sasaki M, Nakamura T
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 4 Pages: 1-15

    • DOI

      10.1371/journal.pone.0195113

    • NAID

      120006987320

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 3テスラ超高b値拡散強調MRIを用いた細胞内拡散係数の推定2021

    • Author(s)
      佐々木美穂、佛坂由可
    • Organizer
      第75回NPO法人日本口腔科学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 高磁場・超高b値拡散強調MRIを用いた 細胞内拡散係数の推定2019

    • Author(s)
      佐々木 美穗、佛坂 由可、片山 郁夫、榮田 智、高木 幸則、角 美佐、中村 卓
    • Organizer
      日本歯科放射線学会 第60回学術大会・第16回定例総会
    • Related Report
      2019 Research-status Report
  • [Presentation] 高磁場・超高b値 拡散強調MRIを用いた細胞内拡散系数の推定2019

    • Author(s)
      佐々木 美穗、佛坂 由可、片山 郁夫、榮田 智、高木 幸則、角 美佐、中村 卓
    • Organizer
      日本歯科放射線学会第60回学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 高磁場・超高b値における拡散強調撮像法2017

    • Author(s)
      佐々木 美穗、佛坂 由可、片山 郁夫、榮田 智、角 美佐、中村 卓
    • Organizer
      日本歯科放射線学会第58回学術大会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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