Genome-wide analysis of epigenetic modification in hippocampus by oral pathogen endotoxin
| Project/Area Number |
17K11681
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
植原 治 北海道医療大学, 歯学部, 助教 (00709248)
太田 亨 北海道医療大学, 健康科学研究所, 教授 (10223835)
道川 誠 名古屋市立大学, 医薬学総合研究院(医学), 教授 (40270912)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 口腔内病原菌内毒素 / 海馬 / エピジェネティクス |
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| Outline of Final Research Achievements |
This study aims to comprehensively observe epigenetic modification in the hippocampus of the brain by the endotoxin Lipopolysaccharide (LPS) derived from the periodontal pathogen Porphyromonas gingivalis. After administration of LPS to mice for 1 month, the hippocampus was isolated and analyzed.As a result, IL-10 was involved in the change in expression of MME, a gene involved in the onset of Alzheimer's disease, and persistent stimulation by LPS was observed in serum. It was suggested that it might be involved in the progression of Alzheimer's disease by causing amyloid accumulation, which leads to a decrease in IL-10 concentration and a decrease in MME.
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| Academic Significance and Societal Importance of the Research Achievements |
歯周病に関わる病原菌が様々な全身疾患の誘因や増悪因子となることが明らかとなっている。近年、歯周病原菌がアルツハイマー病の発症増悪に関与することが示唆されてきているが、そのメカニズムは未だに明らかとなっていない。本研究は、口腔内細菌のアルツハイマー病の発症、進行への関与とその病態の解明につながる極めて独創的な研究である。これらの研究成果は、病態の発症・進行に関わる新たな原因の究明に貢献するのみならず、エピジェネティクスを対象とした創薬、いわゆるエピゲノム創薬のためのデータとしても活用できるものと考えられる。
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Report
(4 results)
Research Products
(5 results)
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[Presentation] Genome-wide Analysis of Dementia-related Genes in Mouse Hippocampus by P. gingivalis -LPS.2019
Author(s)
Morikawa T, Uehara O, Harada F, Hiraki D, Onishi A, Takai R, Yoshida K, Sato J, Nishimura M, Chiba I, Michikawa M, Abiko A.
Organizer
IADR 97th General Session and Exhibition
Related Report
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[Presentation] マウス海馬のP.gingivalis由来LPS投与による遺伝子発現の網羅的解析2018
Author(s)
森川 哲郎, 植原 治, 原田 文也, Durga Paudel, 大西 綾, 平木 大地, Puja Neopane, Bhoj Raj Adhikari, 宇津宮 雅史, 吉田 光希, 佐藤 惇, 西村 学子, 安彦 善裕
Organizer
第28回日本口腔内科学会・第31回日本口腔診断学会
Related Report
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[Presentation] Genome-wide analysis of the gene related to Alzheimer’s disease in mouse hippocampus stimulated with Lipopolysaccharide derived from P. gingivalis.2017
Author(s)
Tetsuro Morikawa, Osamu Uehara, Fumiya Harada, Durga Paudel, Aya Onishi, Daichi Hiraki,Puja Neopane, Bhoj Raj Adhikari, Masafumi Utsunomiya, Koki Yoshida, Jun Sato,Michiko Nishimura and Yoshihiro Abiko
Organizer
第65回国際歯科研究学会日本部会総会・学術大会
Related Report
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