Role of oral-care in inflammatory bowel disease patients in remission: an oral and intestinal bacteria-based study

• Project/Area Number: 17K11688
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Tsurumi University
• Principal Investigator: MATIN Khairul 鶴見大学, 歯学部, 非常勤講師 (00372433)
• Co-Investigator(Kenkyū-buntansha): 松岡 克善 東京医科歯科大学, 大学院医歯学総合研究科, 寄附講座准教授 (40307393) ; 藤井 俊光 東京医科歯科大学, 医学部附属病院, 助教 (30547451) ; 岡田 彩子 鶴見大学, 歯学部, 助教 (60515584) ; 田上 順次 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (50171567) ; 花田 信弘 鶴見大学, 歯学部, 教授 (70180916)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 炎症性腸疾患 / 寛解期 / 口腔ケア / 口腔内細菌 / 腸内細菌 / 次世代シーケンス / 口腔保健指導 / 腸内細菌叢 / 口腔病原細菌 / 次世代シークエンス解析 / real-time PCR / 口腔細菌叢 / 次世代シーケンス解析 / 歯学 / 腸管細菌叢

Outline of Final Research Achievements

Oral health condition has really come into focus in recent years with growing attention for possible involvement of oral pathogens with the inflammatory bowel disease (IBD) condition. This clinical study investigated effect of an advanced oral-health care on the conditions of IBD patients in remission. Before and after a 12-week of this oral-care program clinical data and bacterial samples were collected. All bacterial samples were sequenced using an advanced next generation sequencing (NGS) technology (16S rRNA sequencing, Ion PGM System). Oral pathogens, namely; Streptococcus sinensis, Streptococcus mutans were detected in most oral and fecal samples and showed a trend of reduction after 12 weeks.
Observing the overall changes including oral-health condition and intestinal flora due to the advanced oral-care it can be suggested that to explore the causative factors in the oral-flora and to elucidate the pathophysiology of IBD continued investigations on this topic would be useful.

Academic Significance and Societal Importance of the Research Achievements

口腔を消化管の1つと認識し、細菌学的にIBD患者の口腔と腸内との関係性に注目した臨床研究は少ない。特に、IBD患者に対して、歯科専門家による専門的口腔ケアの介入を実施し、その前後による口腔・腸内細菌叢の質的・量的変化について検証した報告は他に見当たらない。本研究結果が、よりエビデンスレベルの高い臨床研究のプロトコール立案に資することを期待する。

Research Products

• [Journal Article] Evaluation of the cariogenic potential of a probiotic candidate strain Lactobacillus gasseri YIT 12321 (2022)
  • Author(s): Okada Ayako、Imai Susumu、Kikuchi Tomohiro、Matin Khairul、Otsuka Ryoko、Terai Tomohiko、Okumura Takekazu、Yamamoto Takatsugu、Hanada Nobuhiro
  • Journal Title: Archives of Oral Biology Volume: 136 Pages: 105364-105364
  • DOI: 10.1016/j.archoralbio.2022.105364
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of silver diamine fluoride preparations on biofilm formation of <i>Streptococcus mutans </i> (2021)
  • Author(s): TAKAHASHI Motoi、MATIN Khairul、MATSUI Naoko、SHIMIZU Miyuki、TSUDA Yuka、UCHINUMA Shigeki、HIRAISHI Noriko、NIKAIDO Toru、TAGAMI Junji
  • Journal Title: Dental Materials Journal Volume: 40 Issue: 4 Pages: 911-917
  • DOI: 10.4012/dmj.2020-341
  • NAID: 130008069848
  • ISSN: 0287-4547, 1881-1361
  • Year and Date: 2021-07-25
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of advanced periodontal self-care in patients with early-stage periodontal diseases on endothelial function: An open-label, randomized controlled trial (2021)
  • Author(s): Okada Ayako、Murata Takatoshi、Matin Khairul、Ariyoshi Meu、Otsuka Ryoko、Yamashita Mamiko、Suzuki Masayuki、Wakiyama Rumi、Tateno Ken、Suzuki Megumi、Aoyagi Hitomi、Uematsu Hiromi、Imamura Akiko、Kosaka Miki、Mizukaki Tomoko、Sato Tsutomu、Kawahara Hiroshi、Hanada Nobuhiro
  • Journal Title: PLOS ONE Volume: 16 Issue: 9 Pages: 0257247-0257247
  • DOI: 10.1371/journal.pone.0257247
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Anti-Demineralization Effects of Dental Adhesive-Composites on Enamel-Root Dentin Junction (2021)
  • Author(s): Lai Yu-Jung、Takahashi Rena、Lin Po-Yen、Kuo Ling、Zhou Yuan、Matin Khairul、Chiang Yu-Chih、Shimada Yasushi、Tagami Junji
  • Journal Title: Polymers Volume: 13 Issue: 19 Pages: 3327-3327
  • DOI: 10.3390/polym13193327
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characteristics of biofilm-induced degradation at resin-dentin interfaces using multiple combinations of adhesives and resins (2021)
  • Author(s): Zhou Yuan、Matin Khairul、Shimada Yasushi、Sadr Alireza、Wang Guoqing、Tagami Junji、Feng Xiping
  • Journal Title: Dental Materials Volume: 37 Issue: 8 Pages: 1260-1272
  • DOI: 10.1016/j.dental.2021.04.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Detection and analysis of early degradation at resin-dentin interface by optical coherence tomography (OCT) and confocal laser scanning microscope (CLSM) (2021)
  • Author(s): Zhou Yuan、Matin Khairul、Shimada Yasushi、Wang Guoqing、Sadr Alireza、Tagami Junji
  • Journal Title: Journal of Dentistry Volume: 106 Pages: 103583-103583
  • DOI: 10.1016/j.jdent.2021.103583
  • Peer Reviewed
• [Journal Article] Effects of a surface prereacted glass?ionomer filler coating material on biofilm formation and inhibition of dentin demineralization (2020)
  • Author(s): Yamamoto Shiori、Sayed Mahmoud、Takahashi Motoi、Matin Khairul、Hiraishi Noriko、Nikaido Toru、Burrow Michael F.、Tagami Junji
  • Journal Title: Clinical Oral Investigations Volume: 25 Issue: 2 Pages: 683-690
  • DOI: 10.1007/s00784-020-03577-x
  • Peer Reviewed / Open Access
• [Journal Article] Effects of mechanical abrasion challenge on sound and demineralized dentin surfaces treated with SDF (2020)
  • Author(s): Sayed Mahmoud、Tsuda Yuka、Matin Khairul、Abdou Ahmed、Martin Kim、Burrow Michael F.、Tagami Junji
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 1-11
  • DOI: 10.1038/s41598-020-77035-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of silver-containing agents on the ultra-structural morphology of dentinal collagen (2020)
  • Author(s): Sayed Mahmoud、Hiraishi Noriko、Matin Khairul、Abdou Ahmed、Burrow Michael F.、Tagami Junji
  • Journal Title: Dental Materials Volume: 36 Issue: 7 Pages: 936-944
  • DOI: 10.1016/j.dental.2020.04.028
  • Peer Reviewed
• [Journal Article] Inhibitory effect of zinc-containing desensitizer on bacterial biofilm formation and root dentin demineralization (2019)
  • Author(s): SAAD Amr、NIKAIDO Toru、ABDOU Ahmed、MATIN Khairul、BURROW Michael F.、TAGAMI Junji
  • Journal Title: Dental Materials Journal Volume: 38 Issue: 6 Pages: 940-946
  • DOI: 10.4012/dmj.2018-352
  • NAID: 130007754592
  • ISSN: 0287-4547, 1881-1361
  • Year and Date: 2019-11-26
• [Journal Article] Assessment of root caries under wet and dry conditions using swept-source optical coherence tomography (SS-OCT) (2018)
  • Author(s): Zhou Yuan, Shimada Yasushi, Matin Khairul, Sadr Alireza, Yoshiyama Masahiro, Sumi Yasunori, Tagami Junji
  • Journal Title: Dental Materials Journal Volume: 37 Issue: 6 Pages: 880-888
  • DOI: 10.4012/dmj.2017-273
  • NAID: 130007538075
  • ISSN: 0287-4547, 1881-1361
  • Year and Date: 2018-11-27
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Confocal Laser Microscopic Images of In Vitro Cariogenic Biofilm and Resulted Demineralized Dentin (2018)
  • Author(s): Khairul Matin, Thwe Zin Ei, Ryoko Otsuka, Ayako Okada, Kenichi Nebuka, Nobuhiro Hanada, Junji Tagami
  • Journal Title: Journal of Oral Health & Dentistry Volume: 1 Pages: 39-41
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of UVB and UVC irradiation on cariogenic bacteria in vitro (2018)
  • Author(s): Uchinuma Shigeki, Shimada Yasushi, Matin Khairul, Hosaka Keiichi, Yoshiyama Masahiro, Sumi Yasunori, Tagami Junji
  • Journal Title: Lasers in Medical Science Volume: 34 Issue: 5 Pages: 1-9
  • DOI: 10.1007/s10103-018-2685-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation of resin infiltration on demineralized root surface: An <i>in vitro</i> study (2017)
  • Author(s): Zhou Y, Matin K, Shimada Y, Sumi Y, Tagami J
  • Journal Title: Dental Materials Journal Volume: 36 Issue: 2 Pages: 195-204
  • DOI: 10.4012/dmj.2016-229
  • NAID: 130005519654
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning. (2017)
  • Author(s): Murata T, Okada A, Matin K, Hanada N.
  • Journal Title: J Vis Exp Volume: 128 Issue: 128
  • DOI: 10.3791/56319
  • Peer Reviewed / Open Access
• [Presentation] Metagenomics of the Oral and Intestinal Microbiome from Volunteers having IBD using an NGS System (2019)
  • Author(s): Khairul Matin, Ryoko Otsuka, Ayako Okada, Takatoshi Murata, Meu Ariyoishi, Toshimitsu Fujii, Kenichi Nebuka, Masayuki Otsuki, Junji Tagami, Mamoru Watanabe, Nobuhiro Hanada
  • Organizer: 第68回日本口腔衛生学会・総会
• [Presentation] 血管内皮機能に対する歯周組織健康状態改善の有効性評価：ランダム化並行群間比較試験. (2019)
  • Author(s): 岡田彩子，村田貴俊，有吉芽生，大塚良子，山下万美子，鈴木 恵，青柳ひとみ, 植松裕美，今村安芸子，佐藤 勉，マティン カイルール，花田信弘.
  • Organizer: 第68回日本口腔衛生学会・総会
• [Presentation] がん治療周術期における口腔内環境の変化―1症例報告 第2報 (2018)
  • Author(s): 大塚良子, 今井奨, 岡田彩子, 有吉芽生, 菊地朋宏, 宮之原真由, 曽我部薫, 村田貴俊, マティンカイルール, 野村義明, 花田信弘, 桃井保子, 熊谷崇
  • Organizer: 第67回日本口腔衛生学会・総会
• [Presentation] 血管内皮機能に対する歯周組織健康状態改善の有効性評価:ランダム化並行群間比較試験(第一報) (2018)
  • Author(s): 有吉芽生, 村田貴俊, 岡田彩子, 大塚良子, 山下万美子, 鈴木恵, 青柳ひとみ, 植松裕美, 今村安芸子, 佐藤勉, マティンカイルール, 花田信弘
  • Organizer: 第67回日本口腔衛生学会・総会
• [Presentation] 喫煙者の口腔ケアと歯周組織の健康状態との関連性について (2018)
  • Author(s): 岡田彩子, 村田貴俊, 有吉芽生, 大塚良子, 山下万美子, 鈴木恵, 青柳ひとみ, 植松裕美, 今村安芸子, 佐藤勉, マティンカイルール, 花田信弘
  • Organizer: 第67回日本口腔衛生学会・総会
• [Presentation] フッ化ジアンミン銀系材料塗布後の歯根面におけるバイオフィルム形成の評価 (2018)
  • Author(s): 高橋基, 松井七生子, マティンカイルール, 清水美透, 平石典子, 二階堂徹, 田上順次
  • Organizer: 特定非営利活動法人日本歯科保存学会 2018年度秋季学術大会（第149回）
• [Presentation] Metagenomics of Oral and Intestinal Bacteria using a Next Generation Sequencing System; Preliminary Report (2018)
  • Author(s): Ryoko Otsuka, Khairul Matin, Ayako Okada, Meu Ariyoshi, Kosuke Tanimoto, Junji Tagami, Nobuhiro Hanada
  • Organizer: The 66th Annual Meeting of Japanese Association for Dental Research
  • Int'l Joint Research
• [Presentation] Transparency in Random Allocation in a Randomized Clinical Trial; Effectiveness of Periodontal Health Improvement on Vascular Endothelial Function (2018)
  • Author(s): Ayako Okada, Takatoshi Murata, Meu Ariyoshi, Ryoko Otsuka, Mamiko Yamashita, Miki Kosaka, Masayuki Suzuki, Ken Tateno, Rumi Wakiyama, Megumi Suzuki, Hitomi Aoyagi, Hiromi Uematsu, Akiko Imamura, Tsutomu Sato, Hiroshi Kawahara, Khairul Matin, Nobuhiro Hanada
  • Organizer: The 66th Annual Meeting of Japanese Association for Dental Research
  • Int'l Joint Research
• [Presentation] 血管内皮機能に対する歯周組織健康状態改善の有効性評価：ランダム化並行群間比較試験（ベースライン分析） (2018)
  • Author(s): 村田貴俊,岡田彩子,有吉芽生,大塚良子,山下万美子,鈴木 恵,青柳ひとみ,植松裕美,今村安芸子,佐藤 勉,マティン カイル－ル,花田信弘.
  • Organizer: 第19回日本口腔機能水学会・学術大会
• [Presentation] 次亜塩素酸電解水のさらなる活用と普及 (2018)
  • Author(s): マティン カイルール,根深研一.
  • Organizer: 第19回日本口腔機能水学会・学術大会
• [Presentation] がん治療周術期における口腔内環境の変化1症例報告 (2017)
  • Author(s): 大塚良子,今井 奬,阿部 繭,岡田彩子,有吉芽生,菊地朋宏,村田貴俊,マティン カイルール,野村義明,花田信弘,桃井保子,熊谷 崇.
  • Organizer: 第66回日本口腔衛生学会・総会
• [Presentation] Liquid Carrier Type 3DSトレーを用いた殺菌洗口液の細菌学的・臨床的効果 (2017)
  • Author(s): 岡田彩子,有吉芽生,曽我部薫,大塚良子,宮之原真由,村田貴俊,マティン カイルール,花田信弘.
  • Organizer: 第66回日本口腔衛生学会・総会