Development of Dental Implants with Innovative Surface Structures by Carbon Nanomaterial Modification

• Project/Area Number: 17K11733
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Hokkaido University
• Principal Investigator: Hirata Eri 北海道大学, 歯学研究院, 助教 (10722019)
• Co-Investigator(Kenkyū-buntansha): 横山 敦郎 北海道大学, 歯学研究院, 教授 (20210627)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: インプラント / カーボンナノ物質 / チタン / マクロファージ / 骨再生 / 骨形成 / カーボンナノホーン / カーボンナノチューブ / 骨 / 生体材料 / 生体適合性 / カーボンナノマテリアル / 国際共同研究

Outline of Final Research Achievements

Surface-modified Ti (CNH/Ti) with CNHs could be prepared by electrophoretic deposition; CNH/Ti promoted early osteogenesis without inhibiting bone formation. These results suggest that CNHs on Ti surface regulate macrophage DNA transcription, repair, and replication, and that the expression of proinflammatory cytokines is down-regulated.In addition, we observed the dynamics of locally implanted SWNTs in the body, suggesting that they did not migrate to other organs and remained at the site of implantation.

Academic Significance and Societal Importance of the Research Achievements

CNHsを表面修飾したTi(CNH/Ti)は，泳動電着によって簡便に作製することが可能となった．CNH/Tiは骨の形成を阻害することなく，初期の骨新生を促進した．CNHsををTiにコートしたCNH/Tiはマクロファージの炎症反応を制御することが明らかになった．また，CNTsの局所埋入から体内への分布はほとんど見られなかった．この特徴を用いて，安全で効果的なインプラントの新たな表面修飾法への応用が期待された．

Research Products

• [Int'l Joint Research] CNRS(フランス)
• [Int'l Joint Research] CNRS(フランス)
• [Int'l Joint Research] フランス国立科学研究所(CNRS)(フランス)
• [Journal Article] Stable aqueous dispersions of carbon nanohorns loaded with minocycline and exhibiting antibacterial activity (2020)
  • Author(s): MaedaYukari、Hirata Eri、Takano Yuta、Sakaguchi Norihito、Ushijima Natsumi、Saeki Ayumi、Kimura Sadahito、Shibata Ken-ichiro、Yudasaka Masako、Yokoyama Atsuro
  • Journal Title: CARBON Volume: - Pages: 36-45
  • DOI: 10.1016/j.carbon.2020.04.040
  • Peer Reviewed
• [Journal Article] Fate of Carbon Nanotubes Locally Implanted in Mice Evaluated by Near-Infrared Fluorescence Imaging: Implications for Tissue Regeneration (2019)
  • Author(s): Hirata Eri、Yudasaka Masako、Ushijima Natsumi、Sakaguchi Norihito、Maeda Yukari、Tanaka Takeshi、Kataura Hiromichi、Yokoyama Atsuro
  • Journal Title: ACS Applied Nano Materials Volume: 2 Issue: 3 Pages: 1382-1390
  • DOI: 10.1021/acsanm.8b02267
  • Peer Reviewed
• [Journal Article] Few-Layer Graphene Kills Selectively Tumor Cells from Myelomonocytic Leukemia Patients (2017)
  • Author(s): Julie Russier, Veronica Leon, Marco Orecchioni, Eri Hirata, Patrizia Virdis, Claudio Fozza, Francesco Sgarrella, Gianaurelio Cuniberti, Maurizio Prato, Esterezquez, Alberto Bianco, Lucia G. Delogu
  • Journal Title: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION Volume: 56 Issue: 11 Pages: 3014-3019
  • DOI: 10.1002/anie.201700078
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Carbon nanomaterials for bone tissue regeneration (2017)
  • Author(s): Hirata Eri
  • Journal Title: Hokkaido Journal of Dental Science Volume: 38 Pages: 99-103
  • NAID: 120006360116
  • Peer Reviewed
• [Presentation] カーボンナノホーンに担持したミノサイクリンの静菌作用と徐放性 (2019)
  • Author(s): 前田由佳利、平田恵理、高田紗理、 木村貞仁、 横山敦郎
  • Organizer: 日本口腔インプラント学会第３９回東北・北海道支部学術大会
• [Presentation] Macrophage behavior on the titanium electrodeposited carbon nanohorn . (2019)
  • Author(s): Sadahito Kimura、 Eri Hirata、 Sari Takada、 Masatoshi Sakairi、 Masako Yudasaka、 Atsuro Yokoyama
  • Organizer: NT19 20th International Conference on the Science and Application of Nanotubes and Low-Dimensional Materials
  • Int'l Joint Research
• [Presentation] カーボンナノホーン修飾チタン上でのマクロファージの挙動 (2019)
  • Author(s): 木村貞仁、平田恵理、 髙田紗理、 坂入正敏、湯田坂雅子、横山敦郎
  • Organizer: 第九回ナノカーボンバイオシンポジウム
• [Presentation] Observation of Carbon Nanotubes Locally Implanted in Mice: Implications for Tissue Regeneration. (2019)
  • Author(s): Eri Hirata、 Masako Yudasaka、 Yukari Maeda、 Takeshi Tanaka、 Hiromichi Kataura、 Atsuro Yokoyama
  • Organizer: 7th International Conference for young chemists.
  • Int'l Joint Research
• [Presentation] Occlusal considerations in dental implant therapy. (2019)
  • Author(s): Atsuro Yokoyama
  • Organizer: The 6th Indonesian Symposium of Implant Dentistry
  • Int'l Joint Research / Invited
• [Presentation] The advantages of carbon nanomaterials for bone tissue engineering. (2019)
  • Author(s): Eri Hirata
  • Organizer: NT19 20th International Conference on the Science and Application of Nanotubes and Low-Dimensional Materials
  • Int'l Joint Research / Invited
• [Presentation] Applications of carbon nanomaterials in bone tissue engineering. (2019)
  • Author(s): Eri Hirata
  • Organizer: CNRS Institut de Biologie Moleculaire et Cellulaire, Laboratoire d'Immunopathologie et Chimie Therapeutique IBMC seminaire.
  • Int'l Joint Research / Invited
• [Presentation] Prognosis and Efficacy of Partial Denture Treatment. (2019)
  • Author(s): Atsuro Yokoyama
  • Organizer: 66th Japanese Association for Dental research.
  • Int'l Joint Research / Invited
• [Presentation] Near-infrared photoluminescent imaging of carbon nanotubes locally implanted in mice. (2018)
  • Author(s): Eri Hirata, Masako Yudasaka, Yukari Maeda, Takeshi Tanaka, Hiromichi Kataura, Atsuro Yokoyama
  • Organizer: the 256th American Chemical Society National Meeting
  • Int'l Joint Research
• [Presentation] Macrophage behavior on the carbon nanohorn coated anodized titanium (2018)
  • Author(s): Sadahito Kimura, Eri Hirata, Sari Takada, Masatoshi Sakairi, Masako Yudasaka, Atsuro Yokoyama
  • Organizer: The 11th Congress of Asian Academy of Osseointegration 2018
  • Int'l Joint Research
• [Presentation] Activation of macrophage on the carbon nanohorn functionalized anodized titanium. (2018)
  • Author(s): Sadahito Kimura, Eri Hirata, Sari Takada, Masatoshi Sakairi, Masako Yudasaka, Atsuro Yokoyama
  • Organizer: The 66th Annual meeting of Japanese Association for Dental Research
  • Int'l Joint Research
• [Presentation] In vivo Kinetics of Carbon Nanotubes Locally Implanted in Mice (2018)
  • Author(s): Eri Hirata, Masako Yudasaka, Yukari Maeda, Takeshi Tanaka, Hiromichi Kataura, Atsuro Yokoyama
  • Organizer: The 66th Annual meeting of Japanese Association for Dental Research
  • Int'l Joint Research
• [Presentation] 静菌作用を付与したカーボンナノホーンの開発. (2018)
  • Author(s): 前田由佳利，平田恵理, 髙田紗理, 坂入正敏, 木村貞仁, 横山敦郎.
  • Organizer: 日本バイオマテリアル学会 北海道ブロック第3回研究会
• [Presentation] 骨伝導性向上を目的としたカーボンナノホーンによる陽極酸化チタンの表面修飾. (2018)
  • Author(s): 木村貞仁, 髙田紗理，坂入正敏，湯田坂雅子，平田恵理，横山敦郎.
  • Organizer: 日本バイオマテリアル学会 北海道ブロック第3回研究会
• [Presentation] カーボンナノホーンのミノサイクリン担持による静菌作用の付与. (2018)
  • Author(s): 前田由佳利，平田恵理, 髙田紗理, 坂入正敏, 木村貞仁, 横山敦郎
  • Organizer: 日本補綴学会第127回学術大会
• [Presentation] カーボンナノホーン修飾陽極酸化チタン上でのマクロファージの活性. (2018)
  • Author(s): 木村貞仁,平田恵理, 髙田紗理, 坂入正敏,湯田坂雅子,横山敦郎.
  • Organizer: 第八回ナノカーボンバイオシンポジウム
• [Presentation] ミノサイクリンを担持したカーボンナノホーンの開発. (2017)
  • Author(s): 前田由佳利，平田恵理，小松原浩実，山本悟，横山敦郎.
  • Organizer: 第47回日本口腔インプラント学会学術大会
• [Presentation] カーボンナノホーン修飾陽極酸化チタン上の骨形成 (2017)
  • Author(s): 髙田紗理，平田恵理，前田由佳利，小松原浩実，山本悟，横山敦郎.
  • Organizer: 第47回日本口腔インプラント学会学術大会
• [Presentation] Biodistribution of carbon nanotubes after local implantation of mice (2017)
  • Author(s): Eri Hirata, Masako Yudasaka, Yukari Maeda, Takeshi Tanaka, Hiromichi Kataura, Atsuro Yokoyama
  • Organizer: 第54回フラーレン・ナノチューブ・グラフェン総合シンポジウム