Role of paratrigeminal nucleus connected to mouth and pharynx on chewing and swallowing functions

• Project/Area Number: 17K11775
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Niigata University
• Principal Investigator: Tsujimura Takanori 新潟大学, 医歯学系, 准教授 (00548935)
• Co-Investigator(Kenkyū-buntansha): 井上 誠 新潟大学, 医歯学系, 教授 (00303131) ; 真柄 仁 新潟大学, 医歯学総合病院, 講師 (90452060)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 三叉神経傍核 / 孤束核 / 嚥下 / ラット / 上皮型ナトリウムチャンネル / 上喉頭神経 / 上皮型ナトリウムイオンチャネル / 歯学 / 生理学 / 脳・神経 / トランスレーショナルリサーチ / 動物

Outline of Final Research Achievements

The aim of this study was to investigate the role of paratrigeminal nucleus (Pa5) on chewing and swallowing functions. The swallowing reflexes evoked by electrical stimulation of superior laryngeal nerve (SLN) was inhibited during electrical stimulation of Pa5. We did not evoke any swallows by the focal microinjection of glutamate receptor agonists and GABAA receptor antagonist into the Pa5. On the other hand, many swallows were evoked following the microinjection of glutamate receptor agonists and GABAA receptor antagonist into the nucleus tractus solitarii. The SLN is a primary carrier of initiation of swallowing evoked by laryngeal mechanical stimulation. Furthermore, epithelial sodium channel is involved in those swallows.

Academic Significance and Societal Importance of the Research Achievements

本研究は，咀嚼嚥下における三叉神経傍核の役割を検証することを目的として，動物を対象として行われた．実験の結果，三叉神経傍核の刺激により嚥下誘発が抑制されることが確認された．三叉神経傍核は三叉神経を介して活性化されることが知られており，顎口腔領域からの入力が三叉神経傍核を活性化して嚥下を制御する可能性が考えられた．また，機械刺激による嚥下誘発に，上皮型ナトリウムチャンネルが関与していることが示唆された．

Research Products

• [Journal Article] Evaluation of the association between orofacial pain and dysphagia (2020)
  • Author(s): Tsujimura Takanori、Inoue Makoto
  • Journal Title: Journal of Oral Science Volume: 62 Issue: 2 Pages: 156-159
  • DOI: 10.2334/josnusd.19-0408
  • NAID: 130007821455
  • ISSN: 1343-4934, 1880-4926
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of the epithelial sodium channel in initiation of mechanically evoked swallows in anaesthetized rats (2019)
  • Author(s): Tsujimura Takanori、Ueha Rumi、Yoshihara Midori、Takei Eri、Nagoya Kouta、Shiraishi Naru、Magara Jin、Inoue Makoto
  • Journal Title: The Journal of Physiology Volume: 印刷中 Issue: 11 Pages: 2949-2963
  • DOI: 10.1113/jp277895
  • Peer Reviewed
• [Journal Article] Involvement of hypoglossal and recurrent laryngeal nerves on swallowing pressure (2018)
  • Author(s): Tsujimura T, Suzuki T, Yoshihara M, Sakai S, Koshi N, Ashiga H, Shiraishi N, Tsuji K, Magara J, Inoue M
  • Journal Title: J Appl Physiol Volume: 印刷中 Issue: 5 Pages: 1148-1154
  • DOI: 10.1152/japplphysiol.00944.2017
  • Peer Reviewed
• [Presentation] Peripheral mechanisms of mechanically evoked swallows (2020)
  • Author(s): Takanori Tsujimura, Makoto Inoue
  • Organizer: The 97th Annual Meeting of the Physiological Society of Japan
  • Int'l Joint Research
• [Presentation] Involvement of the epithelial sodium channel in initiation of mechanically evoked swallows in anaesthetized rats (2019)
  • Author(s): Takanori Tsujimura, Makoto Inoue
  • Organizer: Neuro2019
  • Int'l Joint Research
• [Presentation] Hypoglossal and recurrent laryngeal nerves are involved in swallowing pressure generation in anesthetized rats (2018)
  • Author(s): Tsujimura T , Inoue M
  • Organizer: Neuroscience 2018
  • Int'l Joint Research
• [Presentation] Modulation of initiation of swallows evoked by continuous laryngeal TRPV1 activation in anesthetized rats (2018)
  • Author(s): Yoshihara M, Suzuki T, Kota N, Tsujimura T, Inoue M
  • Organizer: Neuroscience 2018
  • Int'l Joint Research
• [Presentation] ENaC is involved in the initiation of mechanically evoked swallow (2018)
  • Author(s): Tsujimura T , Yoshihara M, Suzuki T, Nagoya K, Inoue M
  • Organizer: 96th General Session of the IADR
  • Int'l Joint Research
• [Presentation] 嚥下誘発におけるカプサイシン感受性神経の役割 (2018)
  • Author(s): 辻村恭憲，辻 光順，井上 誠
  • Organizer: 第41回嚥下医学会学術講演会
  • Invited
• [Presentation] Involvement of NMDA and non-NMDA glutamate receptors in the rostral-commissural, medial and ventrolateral NTS subnuclei in the initiation of swallows in anesthetized guinea pigs (2017)
  • Author(s): Tsujimura T, Tsuji K, Canning BJ, Inoue M
  • Organizer: 7th Congress of European Society for Swallowing Disorders
  • Int'l Joint Research