Activation of AMP kinase with masticatory muscle activity enhances osseointegration of implants

• Project/Area Number: 17K11792
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Fukuoka Dental College
• Principal Investigator: Kido Hirofumi 福岡歯科大学, 口腔歯学部, 教授 (90169897)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: インプラント / AMPキナーゼ活性 / 骨結合 / 骨芽細胞 / 分化 / 石灰化 / AMPキナーゼ / 咀嚼筋活動 / オッセオインテグレーション / プラスチックインプラント / チタンコーティング / 動物実験 / 組織形態学的研究 / 形態組織学的研究 / AMPK / キナーゼ活性 / 咀嚼運動

Outline of Final Research Achievements

The effect of AICAR on osseointegration of implants was investigated. Osteoblast-like cell MC3T3-E1 was cultured on titanium plates. Samples were collected on days 0, 7, 14, and 21 for (1) normal culture group, (2) calcification-induced group, and (3) calcification-induced + AMPK activation group. Results: The expression of ALP in the calcification-induced + AMPK-activated group was significantly increased on day 7 compared to the other two groups. Gene expression of osterix was also significantly increased in the calcification-induced + AMPK-activated group compared to the other two groups on day 21. Furthermore, ALP activity was significantly increased in the calcification-induced + AMPK-activated group on days 7 and 14 compared to the other two groups.

Academic Significance and Societal Importance of the Research Achievements

本研究では咀嚼筋の活動によって引き起こされるAMPキナーゼ活性がチタン製の歯科インプラントと骨の結合を強化することを証明しようとしたものである。チタン版上で骨芽細胞を培養し、通常の培養と骨分化誘導を行ったものと、AMPキナーゼの活性処理を行ったものを比較したところ、AMPキナーゼ活性を誘導した群で、骨芽細胞の分化が誘導された。このことから、AMPキナーゼが活性される条件下で、インプラントと骨の結合が強化されることが示唆された。

Research Products

• [Journal Article] The effect of AMP kinase activation on differentiation and maturation of osteoblast cultured on titanium plate. (2022)
  • Author(s): Phanthavong V, Kakura K, Taniguchi Y, Egashira K, Matsuzaki E, Tsutsumi T, Kido H
  • Journal Title: Journal of Dental Science Volume: in press
  • Peer Reviewed / Open Access
• [Presentation] Circumvention of posterior alveolar artery in maxillary sinus elevation: A digital approach (2018)
  • Author(s): M. Otawa, K. Yasumatsu, E. Nakamura, K. Kakura, H. Kido
  • Organizer: Academy of Osseointegration 2018 Annual meeting
  • Int'l Joint Research
• [Presentation] Biomaterials-induced neuronal PAS domain2 (Npas2) in bone marrow environment facilitated enhanced osseointegration of titanium implant with complex surface modification (2018)
  • Author(s): K. Morinaga, S. Park, A. Hokugo, H. Sasaki H. Kido, I. Nishimura
  • Organizer: Academy of Osseointegration 2018 Annual meeting
  • Int'l Joint Research