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Developmental research on maxillofacial bone regenerative therapy using super-selectively derived dental pulp stem cells

Research Project

Project/Area Number 17K11803
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dental engineering/Regenerative dentistry
Research InstitutionShimane University

Principal Investigator

Kanno Takahiro  島根大学, 学術研究院医学・看護学系, 教授 (60633360)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords口腔顎顔面外科 / 顎骨再生 / 幹細胞 / 生体材料 / 顎骨再建 / 骨芽細胞 / 生体吸収材料 / 足場材料 / 骨再生足場材料 / 間葉系幹細胞 / 歯髄幹細胞 / 骨髄幹細胞 / 骨伝導能 / 骨再生 / 歯髄由来幹細胞 / 骨髄由来幹細胞
Outline of Final Research Achievements

The maxillofacial boney and soft-tissue defects following oral and maxillofacial tumor and trauma can cause loss of oral function and esthetics. They need oral and maxillofacial reconstructive surgery by using a tissue engineering and bioregenerative treatment approach. With this scientific grant, I tried to develop an innovative maxillofacial boney regenerative treatment using super-selectively derived dental pulp stem cells and bioactive/bioresorbable 3D-porous-uHA-poly-DL-lactide composite scaffold (3D-uHA/PDLLA). I applied these human super-selectively derived dental pulp stem cells into 3D-uHA/PDLLA scaffold and grafted them into rat mandibular critical bone defect models. The results obtained revealed that the grafted models tended to show more boney regeneration in rats' mandibular defects, but not statisticaly significant compared with control groups. The further research remains needed to identify feasible grafting conditions and to develop feasible bone graft scaffolds.

Academic Significance and Societal Importance of the Research Achievements

歯髄由来幹細胞は、抜去された歯から入手されるため、容易かつ侵襲が少なく、再生医療へ有用性が高いと考えます。従来までの顎骨再生への臨床応用への問題点としては、より骨再生能の高い幹細胞群のみを選択的に単離し、細胞リソースとする点が困難でした。われわれは、選択的に骨再生能に有用な幹細胞群を誘導できる可能性を探索することが出来ました。さらに、顎骨特有な再生の足場材料として3次元多孔質u-HA/PDLLA複合体が有用である可能性を明らかとしました。今回の動物実験モデルからは、それらを組み合わせることによる有意性までは証明ができませんでしたが、今後の研究発展に向けた高い発展性を有する成果が上げられました。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (30 results)

All 2020 2019 2018 2017

All Journal Article (15 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 15 results,  Open Access: 12 results) Presentation (15 results) (of which Int'l Joint Research: 10 results,  Invited: 11 results)

  • [Journal Article] Comparison of the bone-regenerative capacity of three-dimensional uncalcined and unsintered hydroxyapatite/poly-D/L-lactide and beta-tricalcium phosphate used as bone graft substitutes.2019

    • Author(s)
      Bai Y, Sha J, Kanno T, Miyamoto K, Hideshima K, Matsuzaki Y
    • Journal Title

      J Invest Surg

      Volume: Epub ahead of print Issue: 3 Pages: 1-14

    • DOI

      10.1080/08941939.2019.1616859

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biomechanical Loading Comparison between Titanium and Unsintered Hydroxyapatite/Poly-L-Lactide Plate System for Fixation of Mandibular Subcondylar Fractures2019

    • Author(s)
      Sukegawa Shintaro、Kanno Takahiro、Yamamoto Norio、Nakano Keisuke、Takabatake Kiyofumi、Kawai Hotaka、Nagatsuka Hitoshi、Furuki Yoshihiko
    • Journal Title

      Materials

      Volume: 12 Issue: 9 Pages: 1557-1557

    • DOI

      10.3390/ma12091557

    • NAID

      120006816641

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A retrospective comparative study of mandibular fracture treatment with internal fixation using reconstruction plate versus miniplates2019

    • Author(s)
      Sukegawa S, Kanno T, Masui M, Sukegawa-Takahashi Y, Kishimoto T, Sato A, Furuki Y
    • Journal Title

      J Craniomaxillofac Surg

      Volume: 47 Issue: 8 Pages: 1175-1180

    • DOI

      10.1016/j.jcms.2018.09.025

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Navigation-Assisted Orbital Trauma Reconstruction Using a Bioactive Osteoconductive/Bioresorbable u-HA/PLLA System2019

    • Author(s)
      Kanno T, Sukegawa S, Karino M, Furuki Y
    • Journal Title

      J Maxillofac Oral Surg

      Volume: 18 Issue: 3 Pages: 329-338

    • DOI

      10.1007/s12663-019-01207-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficacy of Bacterial Cellulose as a Carrier of BMP-2 for Bone Regeneration in a Rabbit Frontal Sinus Model2019

    • Author(s)
      Koike T, Sha J, Bai Y, Matsuda Y, Hideshima K, Yamada T, Kanno T
    • Journal Title

      Materials (Basel)

      Volume: 12 Issue: 15 Pages: 2489-2489

    • DOI

      10.3390/ma12152489

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly l-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study.2019

    • Author(s)
      Dong QN, Kanno T, Bai Y, Sha J, Hideshima K
    • Journal Title

      Materials (Basel)

      Volume: 12 Issue: 18 Pages: 2931-2931

    • DOI

      10.3390/ma12182931

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Advantage of Alveolar Ridge Augmentation with Bioactive/Bioresorbable Screws Made of Composites of Unsintered Hydroxyapatite and Poly-L-lactide2019

    • Author(s)
      Sukegawa Shintaro、Kawai Hotaka、Nakano Keisuke、Takabatake Kiyofumi、Kanno Takahiro、Nagatsuka Hitoshi、Furuki Yoshihiko
    • Journal Title

      Materials

      Volume: 12 Issue: 22 Pages: 3681-3681

    • DOI

      10.3390/ma12223681

    • NAID

      120006816642

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bioactive/Bioresorbable Three-Dimensional Porous Uncalcined and Unsintered Hydroxyapatite/Poly-D/L-lactide Composite with Human Mesenchymal Stem Cells for Bone Regeneration in Maxillofacial Surgery: A Pilot Animal Study2019

    • Author(s)
      Jingjing Sha, Takahiro Kanno, Kenichi Miyamoto, Yunpeng Bai, Katsumi Hideshima, Yumi Matsuzaki
    • Journal Title

      Materials

      Volume: 12 Issue: 5 Pages: 705-705

    • DOI

      10.3390/ma12050705

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Overview of innovative advances in bioresorbable plate systems for oral and maxillofacial surgery2018

    • Author(s)
      Takahiro Kanno, Shintaro Sukegawa, Yoshihiko Furuki, Yoshiki Nariai, Joji Sekine
    • Journal Title

      Japanese Dental Science Review

      Volume: 54 Issue: 3 Pages: 127-138

    • DOI

      10.1016/j.jdsr.2018.03.003

    • NAID

      210000165299

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Application of computer-assisted navigation systems in oral and maxillofacial surgery2018

    • Author(s)
      Shintaro Sukegawa, Takahiro Kanno, Yoshihiko Furuki
    • Journal Title

      Japanese Dental Science Review

      Volume: 54 Issue: 3 Pages: 139-149

    • DOI

      10.1016/j.jdsr.2018.03.005

    • NAID

      210000170222

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Feasibility of a three-dimensional porous uncalcined and unsintered hydroxyapatite/poly-D/L-lactide composite for the regenerative biomaterial in maxillofacial surgery2018

    • Author(s)
      Yunpeng Bai, Takahiro Kanno, Hiroto Tatsumi, Kenichi Miyamoto, Jingjing Sha, Katsumi Hideshima, Yumi Matsuzaki
    • Journal Title

      Materials

      Volume: 11 Issue: 10 Pages: 2047-2047

    • DOI

      10.3390/ma11102047

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Retrospective clinical study of dental implants following sinus floor elevation: An up-to-10-year follow-up evaluation2018

    • Author(s)
      Takahiro Kanno, Shintaro Sukegawa, Masanori Masui, Yuka Sukegawa-Takahashi, Tsukasa Kishimoto, Joji Sekine, Yoshihiko Furuki
    • Journal Title

      Journal of Bio-Integration,

      Volume: 8 Pages: 83-93

    • NAID

      130007877858

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biomechanical loading evaluation of unsintered hydroxyapatite/poly-L-lactide plate system in bilateral sagittal split ramus osteotomy.2017

    • Author(s)
      Shintaro Sukegawa, Takahiro Kanno, Yoshiki Manabe, Kenichi Matsumoto, Yuka Sukegawa-Takahashi, Masanori Masui, Yoshihiko Furuki
    • Journal Title

      Materials

      Volume: 10 Issue: 7 Pages: 764-764

    • DOI

      10.3390/ma10070764

    • NAID

      120006540135

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Precision of Post-Traumatic Orbital Reconstruction Using Unsintered Hydroxyapatite Particles/Poly-L-Lactide Composite Bioactive/Resorbable Mesh Plate with and without Navigation: A Retrospective Study2017

    • Author(s)
      Shintaro Sukegawa, Takahiro Kanno, Yuta Koyama, Kenichi Matsumoto, Yuka Sukegawa-Takahashi, Masanori Masui, Shigeto Tanaka, Yoshihiko Furuki
    • Journal Title

      Journal of Hard Tissue Biology

      Volume: 26 Issue: 3 Pages: 274-280

    • DOI

      10.2485/jhtb.26.274

    • NAID

      130005745400

    • ISSN
      1341-7649, 1880-828X
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Computer-assisted Secondary Reconstruction of Mandibular Continuity Defects Using Non-Vascularized Iliac Crest Bone Graft Following Oral Cancer Resection2017

    • Author(s)
      Takahiro Kanno, Masaaki Karino, Aya Yoshino, Takashi Koike, Hiroto Tatsumi, Koji Tsunematsu, Yoshiki Nariai, Taichi Ide, Yunpeng Bai, Ngoc Quang Dong, Joji Sekine
    • Journal Title

      Journal of Hard Tissue Biology

      Volume: 26 Issue: 4 Pages: 386-392

    • DOI

      10.2485/jhtb.26.386

    • NAID

      130006832130

    • ISSN
      1341-7649, 1880-828X
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Japanese Up-to-date Navigation- and Computer-Assisted Orbital Trauma Reconstruction2020

    • Author(s)
      Takahiro Kanno
    • Organizer
      The First Indo-Japan Multidisciplinary International Symposium, South Asia Society of Oral Maxillofacial Implantology (SASOMI)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ヒト骨髄間葉系幹細胞と生体活性3次元多孔質u-HA/PDLLA複合体を用いた顎骨再生療法2019

    • Author(s)
      管野貴浩,Bai yunpeng, Sha Jingjing, 狩野正明, 小池尚史, 秀島克巳
    • Organizer
      第73回日本口腔科学会総会・学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Navigation- and Computer-Assisted Orbital Trauma Reconstruction2019

    • Author(s)
      Takahiro Kanno
    • Organizer
      The 7th Biennial International Congress of Advanced Digital Technology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Feasibility of an unsintered hydroxyapatite particles/poly-L-lactide composite sheet for navigation-assisted orbital trauma reconstruction2019

    • Author(s)
      Takahiro Kanno
    • Organizer
      第69回ドイツ口腔顎顔面外科学会総会・学術大会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Bone augmentation for functional and esthetic oral rehabilitation using dental implants following oral and maxillofacial trauma2019

    • Author(s)
      Takahiro Kanno
    • Organizer
      第64回日本口腔外科学会総会・学術大会 国際シンポジウム6
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] サイナスリフト後の長期経過 -術式と移植材料-2018

    • Author(s)
      管野貴浩
    • Organizer
      第8回日本バイオインテグレーション学会総会・学術大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Feasibility of an Unsintered Hydroxyapatite Particles/Poly-L-Lactide Composite Sheet for Navigation-Assisted Orbital Trauma Reconstruction2018

    • Author(s)
      Takahiro Kanno, Masaaki Karino, Koji Tsunematsu, Hiroto Tatsumi, Aya Yoshino, Takashi Koike, Taichi Ide, Joji Sekine
    • Organizer
      第64回国際外科学会・日本支部総会・学術大会
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Feasibility of an u-HA/PLLA composite sheet for navigation assisted orbital trauma reconstruction2018

    • Author(s)
      Takahiro Kanno
    • Organizer
      The 43rd Annual Conference of Association of Oral and Maxillofacial Surgeons of India (AOMSI)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 下顎骨関節突起骨折の手術治療 ー その適応と限界 ー2018

    • Author(s)
      管野貴浩
    • Organizer
      第63回日本口腔外科学会総会・学術大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Functional and aesthetic oral rehabilitation using dental implant following alveolar regenerative augmentation for atrophic alveolar ridge of the anterior jaw2018

    • Author(s)
      Takahiro Kanno
    • Organizer
      The 22nd Conference of Japan Academy of MaxilloFacial Implant ~Pan-Pacific Implant Society, Winter Meeting 2018 in TOKYO 2~
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] The up-to-date feasibility of “Computer-Assisted Digital Orthognathic Surgery”2017

    • Author(s)
      Takahiro Kanno, Joji Sekine
    • Organizer
      第58回大韓口腔顎顔面外科学会総会・学術集会
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Feasibility of an Unsintered Hydroxyapatite Particles/Poly-L-Lactide Composite Sheet for Navigation-Assisted Orbital Fracture Reconstruction2017

    • Author(s)
      Takahiro Kanno, Masaaki Karino, Aya Yoshino, Takashi Koike, Taichi Ide, Hiroto Tatsumi, Koji Tsunematsu, Hideki Yoshimatsu1,Joji Sekine
    • Organizer
      第71回日本口腔科学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] 眼窩骨折における生体活性を有するバイオセラミックス微粒子配合 ポリ-L-乳酸シートを用いた眼窩再建治療2017

    • Author(s)
      管野貴浩、狩野正明、辰巳博人、金子一朗、吉松英樹、井出太一、高橋尊史、吉野綾、関根浄治
    • Organizer
      第19回日本口腔顎顔面外傷学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Feasibility of an Unsintered Hydroxyapatite Particles/Poly-L-Lactide Composite Sheet for Navigation-Assisted Orbital Fracture Reconstruction2017

    • Author(s)
      Takahiro Kanno, Joji Sekine
    • Organizer
      第62回日本口腔外科学会総会・学術大会
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] The Current Trends of Maxillofacial Trauma Surgery: Focusing on “Orbital Fracture Reconstruction”2017

    • Author(s)
      Takahiro Kanno, Joji Sekine
    • Organizer
      2nd Takao International Forum of Oral and Maxillofacial Surgery
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2017-04-28   Modified: 2021-02-19  

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