Analysis of mechanical stress-mediated control mechanism of cancer stem cell character in oral squamous cell carcinoma.
| Project/Area Number |
17K11837
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
Shigeishi Hideo 広島大学, 医系科学研究科(歯), 講師 (90397943)
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| Co-Investigator(Kenkyū-buntansha) |
太田 耕司 広島大学, 医系科学研究科(歯), 教授 (20335681)
小野 重弘 広島大学, 医系科学研究科(歯), 助教 (70379882)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 癌幹細胞 / 口腔癌 / ハイドロゲル / 細胞外基質 / ラミニン332 / 口腔扁平上皮癌 |
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| Outline of Final Research Achievements |
We investigated changes in phenotype in CD44 high-oral squamous cell carcinoma cells that were cultured on laminin332-coated hydrogel with various degrees of stiffness. Mesenchymal-like morphology was observed when cells were cultured on 4.0 kPa laminin-coated hydrogel; amoeboid-like morphology was observed when cells were cultured on 1.0 kPa laminin-coated hydrogel. These results indicated that CD44high cells underwent mesenchymal to amoeboid transition when cultured on laminin-coated softer hydrogel.
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| Academic Significance and Societal Importance of the Research Achievements |
CD44high口腔扁平上皮癌細胞は、ラミニン332にてコーテイングしたハイドロゲル上にて培養した結果、足場の弾性率の変化によりamoeboid様形態を示す細胞に変化することが明らかとなった。さらに、アクチン細胞骨格を制御するcofilin-1がamoeboid様細胞の形態維持に必要であることが示唆された。このことから、口腔癌幹細胞は周囲の細胞外基質や足場の弾性率に応じて、細胞形態や遺伝子発現パターンを柔軟に変化させることが可能であり、表現型および遺伝子発現の可塑性が口腔癌幹細胞の重要な特徴のひとつであることが明らかとなった。
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] CD44high/ESAlow squamous cell carcinoma cell-derived prostaglandin E2 confers resistance to 5-fluorouracil-induced apoptosis in CD44high/ESAhigh cells2018
Author(s)
Shigeishi H, Hashikata M, Yokoyama S, Sakuma M, Murozumi H, Kato H, Rahman MZ, Seino S, Ishioka Y, Ohta K, Takechi M, Sugiyama M
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Journal Title
Int J Clin Exp Pathol
Volume: 11
Pages: 2356-2363
Related Report
Peer Reviewed
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