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The Changes in multipotency of bone tissue-derived mesenchymal cells by donor site and its clinical significance.

Research Project

Project/Area Number 17K11852
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionKitasato University

Principal Investigator

Yamazaki Yasuharu  北里大学, 医学部, 非常勤講師 (00210401)

Co-Investigator(Kenkyū-buntansha) 杉本 孝之  北里大学, 医学部, 講師 (20365133)
馬場 香子  北里大学, 医学部, 講師 (90327411)
杉本 佳香  北里大学, 医学部, 助教 (90775941)
森山 和の  北里大学, 医学部, 助教 (40803515)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords未分化間葉系細胞 / 再生医工学 / エクソソーム / 神経堤由来細胞 / 神経堤由来間葉系細胞
Outline of Final Research Achievements

Biological characteristics and next generation RNA-sequencing analysis of human bone-derived mesenchymal cells (MSCs) depending on whether they are derived from iliac bone or maxillofacial bone, then exosomes in the culture supernatant during induction of bone differentiation of MSCs, moreover the changes of long-term cryopreservation MSCs were investigated. As a result, in biological characteristics, a difference in the differentiation ability of MSCs depending on the collection site was observed, and next generation RNA-sequencing analysis revealed that most of the iliac MSCs DEGs are HOX genes. It was suggested that exosomes may be related to the environment of the transplant bed and cell influx. In addition, aging due to long-term cryopreservation may not have progressed, and long-term cryopreservation MSCs may be useful for basic research.

Academic Significance and Societal Importance of the Research Achievements

顎骨由来間葉系細胞は主に膜性骨化、腸骨由来間葉系細胞は主に軟骨内骨化の形態をたどる.それは発生学的には顎骨由来間葉系細胞は神経堤由来であり腸骨由来間葉系細胞は中胚葉由来と異なるためで、そのことから顎骨由来間葉系細胞と腸骨由来間葉系細胞との分化誘導能に差異が生じていると考えられた.従って臨床での骨移植再建の際はこれらの性質を十分に理解し、より適した部位をドナーとして選択する事が望ましいと思われた.

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (13 results)

All 2020 2019 2018 2017

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (10 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Difference in pluripotency of jaw and iliac bone-derived mesenchymal cells.2020

    • Author(s)
      Kazuno Moriyama, Yasuharu Yamazaki, Yoshika Sugimoto, Takayuki Sugimoto, Kenichi Kumazawa, Kyoko Baba, Yumiko Sone, Akira Takeda
    • Journal Title

      The Kitasato Medical Journal

      Volume: 50 Pages: 18-26

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An in vitro long-term study of cryopreserved umbilical cord blood-derived platelet-rich plasma containing growth factors―PDGF-BB, TGF-β, and VEGF2019

    • Author(s)
      Kyoko Baba, Yasuharu Yamazaki, Yumiko Sone, Yoshika Sugimoto, Kazuno Moriyama, Takayuki Sugimoto, Kennichi Kumazawa, Yasuhito Shimakura, Akira Takeda
    • Journal Title

      Journal of Cranio-Maxillo-Facial Surgery

      Volume: 47 Issue: 4 Pages: 668-675

    • DOI

      10.1016/j.jcms.2019.01.020

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vascular endothelial growth factor receptor 1 (VEGFR1) tyrosine kinase signaling facilitates granulation tissue formation with recruitment of VEGFR1+ cells from bone marrow2017

    • Author(s)
      Park Keiichi、Amano Hideki、Ito Yoshiya、Mastui Yoshio、Kamata Mariko、Yamazaki Yasuharu、Takeda Akira、Shibuya Masabumi、Majima Masataka
    • Journal Title

      Anatomical Science International

      Volume: 印刷中 Issue: 3 Pages: 1-7

    • DOI

      10.1007/s12565-017-0424-8

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 20年長期凍結保存した骨由来間葉系細胞の分化能・増殖能及び長期継代を繰り返した細胞との老化染色の検討2020

    • Author(s)
      杉本佳香、山崎安晴,森山和の、杉本孝之,熊澤憲一,馬場香子、曽根由美子,武田啓
    • Organizer
      日本再生医療学総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シュワン細胞との共培養によるヒト臍帯由来間葉系細胞の分化能の検討2020

    • Author(s)
      馬場 香子、山崎 安晴 , 曽根 由美子, 杉本 佳香 , 森山 和の , 杉本 孝之 , 熊澤 憲一, 武田 啓
    • Organizer
      日本再生医療学総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 長期凍結保存した骨由来間葉系細胞の分化能・増殖能の検討ー20年、15年、10年、5年、1年の保存検体を比較してー2019

    • Author(s)
      杉本佳香、山崎安晴,森山和の、杉本孝之,熊澤憲一,馬場香子、曽根由美子,武田啓
    • Organizer
      日本形成外科学会基礎学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 共培養で得られた臍帯間葉系細胞由来再生軟骨の組織学的評価2019

    • Author(s)
      馬場香子、山崎安晴、杉本佳香、森山和の、杉本孝之、熊澤憲一、武田 啓
    • Organizer
      第18回日本再生医療学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] 骨組織由来間葉系細胞の分化能・増殖能・老化現象の確認 ~ 20 年・15 年・10 年・5 年・1 年の保存検体を用いて~2019

    • Author(s)
      杉本佳香、山崎安晴、森山和の、杉本孝之、熊澤憲一、馬場香子、曽根由美子、武田 啓
    • Organizer
      第18回日本再生医療学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] 顎骨由来および腸骨由来間葉系細胞の多分化能の差異について2019

    • Author(s)
      森山和の、山崎安晴、杉本佳香、杉本孝之、熊澤憲一、馬場香子、曽根由美子、武田 啓
    • Organizer
      第18回日本再生医療学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] 当科が選択したヒト腸骨由来間葉系細胞培養上清のエクソソーム精製法とその骨分化誘導能の検討2018

    • Author(s)
      杉本 佳香、山崎 安晴、杉本 孝之、森山 和の、馬場 香子、熊澤 憲一、曽根 由美子、武田 啓
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Biological differences of human mesenchymal cells derived from different bone tissues and comprehensive gene expression analysis.2018

    • Author(s)
      Yasuharu Yamazaki, Yoshika Sugimoto, Kazuno Moriyama, Kyoko Baba, Yumiko Sone, Takayuki Sugimoto, Takanori Iwata, Satoru Onizuka, and Akira Takeda
    • Organizer
      Society For Biomaterials,2018 Annual Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Examination of Exosome purification method derived from culture supernatant of the mesenchymal cells from human ilium bone(hBMCs) which we chose, and its bone differentiation inducibility.2018

    • Author(s)
      Yoshika Sugimoto, Yasuharu Yamazaki, Kazuno Moriyama, Kyoko Baba, Yumiko Sone, Takayuki Sugimoto, Kenichi Kumazawa, and Akira Takeda
    • Organizer
      Society For Biomaterials,2018 Annual Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 顎骨vs腸骨由来間葉系細胞の生物学的差異と網羅的遺伝子発現解析2017

    • Author(s)
      山崎安晴、杉本佳香、森山和の、曽根由美子、馬場香子、杉本孝之、鬼塚 理、岩田隆紀、武田 啓
    • Organizer
      第26回日本形成 外科学会基礎学術集会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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