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Analysis of invasion and metastasis in oral cancer using human a three-dimensional cultured tissue model

Research Project

Project/Area Number 17K11872
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionOsaka University

Principal Investigator

Iwai Soichi  大阪大学, 歯学部附属病院, 講師 (10362675)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsヒト3次元組織体モデル / 癌の浸潤・転移 / 組織工学 / ヒト3次元組織体モデル / 3次元組織モデル / 癌 / 浸潤・転移
Outline of Final Research Achievements

Preventing cancer metastasis requires a thorough understanding of cancer cell invasion. In vivo transplantation in animals is typically have some problems.In vitro conventional analysis of migration/invasion ability based on 2D culture methods may not reflect actual events that occur in living tissues, because these phenomena occur in human 3D living tissues.
We developed a human cell-based 3D-cultured tissue constructs with blood/lymphatic capillaries that imitate in vivo human tissue organization by which it can be used to analyze the invasion of human oral squamous cell carcinoma cells. This system allowed detailed analysis of cancer cell migration and invasion in an environment that mimics actual human tissue. Moreover, we developed further the construction of epithelialized 3D-cell cultured tissue system. The constructed epithelialized 3D cell culture system with blood capillaries are useful for vitro/ex vivo research models of the analysis of tumor cell invasion.

Academic Significance and Societal Importance of the Research Achievements

口腔癌の浸潤・転移のメカニズムの解明は、がんの治療薬開発に繋がる重要な研究である。今まで癌細胞の遊走・浸潤過程を、実験動物への癌細胞の移植実験や2次元培養法による解析を進めてきた。しかし、動物実験は異種移植でありヒト組織内環境を反映せず、2次元培養法による解析は3次元生体組織を反映していない点がある。我々が開発した血管及びリンパ管様組織を伴ったヒト3次元組織体モデルを癌細胞の浸潤・遊走の解析に用い、従来の方法と比較して、より生体に近い解析方法であることを示すことができた。さらに、3次元組織体に上皮細胞を積層し、口腔粘膜上皮を模倣した組織を開発した。これは癌浸潤を解析する有用なツールとなり得る。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (10 results)

All 2020 2019 2018 2017

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Construction of Vascularized Oral Mucosa Equivalents Using a Layer-by-layer Cell Coating Technology2019

    • Author(s)
      Kyoko Nishiyama, Takami Akagi, Soichi Iwai, Mitsuru Akashi
    • Journal Title

      Tissue Engineering, Part C Methods

      Volume: 25 Issue: 5 Pages: 262-275

    • DOI

      10.1089/ten.tec.2018.0337

    • NAID

      40022817633

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional cultured tissue constructs that imitate human living tissue organization for analysis of tumor cell invasion2017

    • Author(s)
      Iwai Soichi、Kishimoto Satoko、Amano Yuto、Nishiguchi Akihiro、Matsusaki Michiya、Takeshita Akinori、Akashi Mitsuru
    • Journal Title

      Journal of Biomedical Materials Research Part A

      Volume: 106A Issue: 2 Pages: 292-300

    • DOI

      10.1002/jbm.a.36319

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ヒト生体組織を模倣した三次元組織体を用いた口腔扁平上皮癌の浸潤能の解析2020

    • Author(s)
      西山今日子、岩井聡一、岸本聡子、森田祥弘、竹下彰範、鵜澤成一
    • Organizer
      第38回日本口腔腫瘍学会学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細胞コーテイング技術を用いた脈管系を含む三次元口腔粘膜モデルの構築2019

    • Author(s)
      西山今日子、岩井聡一、赤木隆美、明石満
    • Organizer
      第18回日本再生医療学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 脈管系を含む三次元生体組織モデルを用いた口腔扁平上皮癌の遊走・浸潤能の解析2018

    • Author(s)
      西山今日子、岩井聡一、岸本聡子、松崎典弥、赤木隆美、明石 満
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2018 Research-status Report 2017 Research-status Report
  • [Presentation] Novel analysis of cancer cell invasion using a three-dimensional cultured tissue model mimics human tissue organization2018

    • Author(s)
      Soichi Iwai, Satoko Kishimoto, Kyoko Nishiyama, Michiya Matsusaki, Takami Akagi, Mitsuru Akashi
    • Organizer
      EACR25 Amsterdam Netherland
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analysis of tumor cell invasion using three-dimensional cultured tissue model with blood/lymph-capillaries network2018

    • Author(s)
      Kyoko Nishiyama, Soichi Iwai, Satoko Kishimoto, Narikazu Uzawa, Mitsuru Akashi
    • Organizer
      第77回日本癌学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Novel analysis system of cancer cell invasion using a three-dimensional cultured tissue model mimics human tissue organization.2018

    • Author(s)
      Soichi Iwai, Satoko Kishimoto, Kyoko Nishiyama, Michiya Matsusaki, Takami Akagi, Mitsuru Akashi
    • Organizer
      EACR-AACR-ISCR Conference The cutting Edge of Contemporary Cancer Research
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細胞コーティング技術を用いた三次元培養口腔歯肉・粘膜モデルの開発2018

    • Author(s)
      西山今日子、岩井聡一、赤木隆美、明石満
    • Organizer
      第40回日本バイオマテリアル学会
    • Related Report
      2018 Research-status Report
  • [Presentation] ヒト組織を模倣したリンパ管ネットワーク付き3次元組織体による口腔扁平上皮癌の遊走能、浸潤能の解析2017

    • Author(s)
      岩井聡一、岸本聡子、西山今日子、濱田正和、松崎典弥、明石満
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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