| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Preventing cancer metastasis requires a thorough understanding of cancer cell invasion. In vivo transplantation in animals is typically have some problems.In vitro conventional analysis of migration/invasion ability based on 2D culture methods may not reflect actual events that occur in living tissues, because these phenomena occur in human 3D living tissues.
We developed a human cell-based 3D-cultured tissue constructs with blood/lymphatic capillaries that imitate in vivo human tissue organization by which it can be used to analyze the invasion of human oral squamous cell carcinoma cells. This system allowed detailed analysis of cancer cell migration and invasion in an environment that mimics actual human tissue. Moreover, we developed further the construction of epithelialized 3D-cell cultured tissue system. The constructed epithelialized 3D cell culture system with blood capillaries are useful for vitro/ex vivo research models of the analysis of tumor cell invasion.
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