Relationship between SPARC (osteonectin) and recurrence of malignant tumor
| Project/Area Number |
17K11885
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Ohu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
前田 豊信 奥羽大学, 歯学部, 准教授 (10382756)
高田 訓 奥羽大学, 歯学部, 教授 (40254875)
加藤 靖正 奥羽大学, 歯学部, 教授 (50214408)
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| Project Period (FY) |
2017-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | がん / SPARC / AP1 / TRPM5 / TAS2R / AP-1 / 悪性腫瘍 / 再発 / がん幹細胞 |
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| Outline of Final Research Achievements |
SPARC (secreted protein acidic and rich in cysteine) is an extracellular matrix protein, and the mature peptide consists of 286 amino acids. However, it has been suggested that SPARC binds to peptides with a leucine zipper. Therefore, we synthesized a recombinant peptide of SPRAC and analyzed its binding to Fos and Jun, which are members of AP-1. The SPARC gene (NM_003118) 3451 bp, with coding region of 912 bp, 5′-UTR of 64 bp, and huge 3′-UTR of 2475 bp. We analyzed the significance of the SPARC 3′-UTR by inserting part or all of the 3′-UTR into an expression vector and analyzing differences in mRNA stabilization and cell phenotype.
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| Academic Significance and Societal Importance of the Research Achievements |
SPARCは分泌タンパクで、骨基質中に多く存在する。SPARCはアパタイトと高い親和性を有するが、体内に広く分布している。一部の腫瘍ではSPARCは浸潤に重要な因子であり、微小環境を制御する可能性が示されている。さらに、SPARCが細胞内に局在することも示されており、未だにSPRACの機能全容について不明な点が多い。
本成果の意義は、以下の3点である。①SPARCの核内機能を示した ②SPARCの非石灰化間葉組織での機能を明示した ③SPARCの3′-UTRの意義について可能性の一部を排除した これらは、SPARCが関与する悪性腫瘍において、新規治療方法開発の有益な基礎知見に質すると考える。
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Report
(6 results)
Research Products
(20 results)
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[Journal Article] TRPM5 mediates acidic extracellular pH signaling and TRPM5 inhibition reduces spontaneous metastasis in mouse B16-BL6 melanoma cells2017
Author(s)
Toyonobu Maeda, Atsuko Suzuki, Kaori Koga, Chihiro Miyamoto, Yojiro Maehata, Shigeyuki Ozawa, Ryu-Ichiro Hata, Yoji Nagashima, Kazuki Nabeshima, Kaoru Miyazaki, Yasumasa Kato
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Journal Title
Oncotarget
Volume: 8
Issue: 45
Pages: 78312-78326
DOI
Related Report
Peer Reviewed / Open Access
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