| Project/Area Number |
17K11925
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
本田 雅規 愛知学院大学, 歯学部, 教授 (70361623)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 末梢神経再生 / 神経再生 / 末梢神経 / 下歯槽神経 / 坐骨神経 / 間葉系幹細胞 / 再生医学 / 細胞・組織、歯学 |
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| Outline of Final Research Achievements |
ResultsThe sensory reflexes of the OCT group were restored to the level of that of the intact group after 15 days. Hematoxylin and eosin staining revealed the cross-linking between the proximal and distal stumps after 2 weeks. After 4 weeks, Luxol Fast Blue and immunohistochemical staining revealed the presence of myelin sheath from the proximal to distal region of the regenerated tissue and S100B staining confirmed the presence of Schwann cells. Interestingly, no myelin sheath was ultrastructurally observed around the regenerated axons at the central region after 2 weeks.Conclusions：These results suggest that OCTs facilitate the recovery of sensory function. Additionally, the non-myelinated axons contributed to the recovery of the sensory function.
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| Academic Significance and Societal Importance of the Research Achievements |
末梢神経の損傷は支配領域の神経組織、結合組織および筋組織の機能的、形態学的変化をもたらす。軸索の断裂を伴う損傷を受けた場合には、自然回復は困難であり、患者のQOLは著しく低下する。このような有害事象が生じた場合には、従来の外科的治療法の適応となるが、高度な技術や長い手術時間が必要である。今回われわれが開発したOriented collagen tube (OCT)による治療は従来の外科的治療法と比較して患者への身体的負担が少なく、末梢神経障害治療の新たなデバイスとなる可能性があると考えられる。
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