Vibration-induced TGF-b1 expression in osteocytes promotes osteoclastogenesis and accelerates experimental tooth movement.

• Project/Area Number: 17K11929
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: Sasaki Kiyo 東北大学, 大学病院, 助教 (70746958)
• Co-Investigator(Kenkyū-buntansha): 福永 智広 東北大学, 大学病院, 講師 (70362994) ; 清流 正弘 東北大学, 歯学研究科, 助教 (80510023)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯の移動 / 振動刺激 / TGF-β1 / TGFβ / 歯学 / メカニカルストレス

Outline of Final Research Achievements

I examined effect of TGF-β1 on vibration-induced acceleration of experimental tooth movement and its underlying mechanisms. In the rat experimental tooth movement, expression of TGF-β1 increased on the compression side in osteocyte.
Administration of TGF-β1 inhibitor in a rat periodontal tissue attenuated the vibration-induced experimental tooth movement. The vibration-increased osteoclast number on the compression sides was decreased by administration of TGF-β1 inhibitor. Vibration increased phosphorylation of TGF-β1 in MLO-Y4 cells after loading vibration.

Academic Significance and Societal Importance of the Research Achievements

本研究により、振動刺激に応答した骨細胞がTGF-β1を介して破骨細胞分化を促し、骨吸収を促進し、歯の移動が亢進する分子メカニズムが明らかになった。これらの成果は、新たな矯正的歯の移動促進法開発へとつながる基盤的知見となりうる。

Research Products

• [Journal Article] Connective tissue growth factor promotes chemotaxis of preosteoblasts through integrin α5 and Ras during tensile force-induced intramembranous osteogenesis (2021)
  • Author(s): Jiang Wei、Takeshita Nobuo、Maeda Toshihiro、Sogi Chisumi、Oyanagi Toshihito、Kimura Seiji、Yoshida Michiko、Sasaki Kiyo、Ito Arata、Takano-Yamamoto Teruko
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 2368-2368
  • DOI: 10.1038/s41598-021-82246-9
  • Peer Reviewed / Open Access
• [Journal Article] Ten-m/Odz3 regulates migration and differentiation of chondrogenic ATDC5 cells via RhoA-mediated actin reorganization. (2021)
  • Author(s): Takano I, Takeshita N, Yoshida M, Seki D, Oyanagi T, Kimura S, Jiang W, Sasaki K, Sogi C, Kawatsu M, Takano-Yamamoto T.
  • Journal Title: J Cell Physiol. Volume: 236 Issue: 4 Pages: 2906-2919
  • DOI: 10.1002/jcp.30058
  • Peer Reviewed / Open Access
• [Journal Article] Vibration enhances osteoclastogenesis by inducing RANKL expression via NF-κB signaling in osteocytes (2019)
  • Author(s): Mayuri Sakamoto, Tomohiro Fukunaga, Kiyo Sasaki, Masahiro Seiryu, Mitsuhiro Yoshizawa, Nobuo Takeshita, Teruko Takano-Yamamoto
  • Journal Title: Bone Volume: 印刷中 Pages: 56-66
  • DOI: 10.1016/j.bone.2019.03.024
  • Peer Reviewed / Open Access
• [Journal Article] Synergistic acceleration of experimental tooth movement by supplementary high-frequency vibration applied with a static force in rats (2017)
  • Author(s): Teruko Takano-Yamamoto, Kiyo Sasaki, Goudarzi Fatemeh, Tomohiro Fukunaga, Masahiro Seiryu, Takayoshi Daimaruya, Nobuo Takeshita, Hiroshi Kamioka, Taiji Adachi, Hiroto Ida & Atsushi Mayama
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 13969-13969
  • DOI: 10.1038/s41598-017-13541-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Vibration-induced TGF-β1 expression in osteocytes promotes osteoclastogenesis and accelerates experimental tooth movement. (2020)
  • Author(s): Kiyo Sasaki, Nobuo Takeshita, Tomohiro Fukunaga, Masahiro Seiryu, Mayuri Sakamoto, Teruko Takano-Yamamoto
  • Organizer: The 9th International Orthodontic Congress
  • Int'l Joint Research
• [Presentation] 振動刺激による歯の移動促進効果における破骨細胞に対するTGFβの役割 (2018)
  • Author(s): 佐々木紀代、福永智広、坂本麻由里、清流正弘、竹下信郎、山本照子
  • Organizer: 日本矯正歯科学会