Elucidation of genetic factors related to root traits

• Project/Area Number: 17K11947
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Showa University
• Principal Investigator: Maki Koutaro 昭和大学, 歯学部, 教授 (80219295)
• Co-Investigator(Kenkyū-buntansha): 芳賀 秀郷 昭和大学, 歯学部, 講師 (00736655) ; 高橋 正皓 昭和大学, 歯学部, 講師 (10736713) ; 山口 徹太郎 神奈川歯科大学, 大学院歯学研究科, 教授 (40384193) ; 中脇 貴俊 昭和大学, 歯学部, 普通研究生 (90783522)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 歯根 / 歯冠 / ゲノム / 遺伝子 / ヒト / 候補遺伝子 / 一塩基多型 / 歯根長 / 歯冠長 / 遺伝因子

Outline of Final Research Achievements

The relationship between crown morphology and human genes has been reported. However, there are no reports on genes related to human tooth length. The purpose of this study was to clarify the relationship between tooth length and polymorphism of growth hormone receptor gene in Japanese population. DNA was collected from saliva, and GHR gene polymorphisms were typed using Taqman genotyping assay. Rs6184 was significantly associated with maxillary incisor root length, maxillary canine root length / tooth length, and lower incisor root length (P <.05). The GHR gene polymorphism (rs6184) was associated with tooth length in the Japanese population.

Academic Significance and Societal Importance of the Research Achievements

歯根発生に関するメカニズムの理解は、歯の移動や歯根吸収など臨床に直接、益することが期待されるにも関わらず歯冠形成に比べて研究報告も少なく、明らかに立ち遅れている。遺伝因子の解明は歯根吸収の病態理解や歯牙の再生に寄与する。

Research Products

• [Int'l Joint Research] 釜山国立大学矯正歯科(韓国)
• [Int'l Joint Research] ピッツバーグ大学口腔生物学(米国)
• [Int'l Joint Research] スエズカナル大学矯正歯科(エジプト)
• [Journal Article] Functional analysis of PTH1R variants found in primary failure of eruption. (2020)
  • Author(s): Izumida E, Suzawa T, Miyamoto Y, Yamada A, Takimoto R, Otsu M, Saito T, Yamaguchi T, Nishimura K, Ohtaka M, Nakanishi M, Yoshimura K, Sasa K, Takimoto R, Uyama R, Shirota T, Maki K, Kamijo R.
  • Journal Title: Journal of Dental Research Volume: 99 Issue: 4 Pages: 429-436
  • DOI: 10.1177/0022034520901731
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A three-dimensional analysis of primary failure of eruption in humans and mice. (2020)
  • Author(s): 5)Tokavanich N, Gupta A, Nagata M, Takahashi A, Matsushita Y, Yatabe M, Ruellas A, Cevidanes L, Maki K, Yamaguchi T, Ono N, Ono W.
  • Journal Title: Oral Dis Volume: 26 Issue: 2 Pages: 391-400
  • DOI: 10.1111/odi.13249
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Three-dimensional evaluation of mandibular volume in patients with cleft lip and palate during the deciduous dentition period. (2020)
  • Author(s): Shrestha A, Takahashi M, Yamaguchi T, Adel M, Furuhata M, Hikita Y, Yoshida H, Nakawaki T, Maki K.
  • Journal Title: Angle Orthod Volume: 90 Issue: 1 Pages: 85-991
  • DOI: 10.2319/112618-831.1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Three-dimensional assessment of the pharyngeal airway in Japanese preschoolers with orofacial clefts. (2020)
  • Author(s): 2)Takahashi M, Yamaguchi T, Lee MK, Suzuki Y, Adel M, Tomita D, Nakawaki T, Yoshida H, Hikita Y, Furuhata M, Tsuneoka M, Nagahama R, Marazita ML, Weinberg SM, Maki K.
  • Journal Title: Laryngoscope Volume: 130 Issue: 2 Pages: 533-540
  • DOI: 10.1002/lary.27957
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Relationship between the maxillofacial skeletal pattern and the morphology of the mandibular symphysis: Structural equation modeling. (2019)
  • Author(s): Ahn MS, Shin SM, Yamaguchi T, Maki K, Wu TJ, Ko CC, Kim YI.
  • Journal Title: Korean J Orthod Volume: 49 Issue: 3 Pages: 170-180
  • DOI: 10.4041/kjod.2019.49.3.170
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Analysis of the relationship between the morphology of the palate and facial skeletal patterns in Class III malocclusion using structural equation modeling. (2019)
  • Author(s): Ahn MS, Shin SM, Choi YS, Wu TJ, Ko CC, Yamaguchi T, Maki K, Yong-Il K.
  • Journal Title: Orthod Craniofac Res Volume: 22 Issue: 2 Pages: 87-92
  • DOI: 10.1111/ocr.12283
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Autocrine regulation of mesenchymal progenitor cell fates orchestrates tooth eruption. (2019)
  • Author(s): Takahashi A, Nagata M, Gupta A, Matsushita Y, Yamaguchi T, Mizuhashi K, Maki K, Ruellas AC, Cevidanes LS, Kronenberg HM, Ono N, Ono W.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 116 Issue: 2 Pages: 575-58
  • DOI: 10.1073/pnas.1810200115
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Comparison of temporomandibular joint shape and size in patients with facial asymmetry. (2019)
  • Author(s): Han S, Shin SM, Choi YS, Kim KB, Yamaguchi T, Maki K, Chung CJ, Kim YI.
  • Journal Title: Oral Radiol Volume: 35 Issue: 3 Pages: 251-259
  • DOI: 10.1007/s11282-018-0344-x
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Comparison of condylar size among different anteroposterior and vertical skeletal patterns using cone-beam computed tomography. (2019)
  • Author(s): Hasebe A, Yamaguchi T, Nakawaki T, Hikita Y, Katayama K, Maki K.
  • Journal Title: Angle Orthod Volume: 89 Issue: 2 Pages: 306-311
  • DOI: 10.2319/032518-229.1
  • Peer Reviewed
• [Journal Article] Orthognathic surgery induces genomewide changes longitudinally in DNA methylation in saliva (2018)
  • Author(s): Yamaguchi Tetsutaro、Hosomichi Kazuyoshi、Takahashi Masahiro、Haga Shugo、Nakawaki Takatoshi、Hikita Yu、Maki Koutaro、Tajima Atsushi
  • Journal Title: Oral Diseases Volume: 25 Issue: 2 Pages: 508-514
  • DOI: 10.1111/odi.12998
  • Peer Reviewed / Int'l Joint Research