| Project/Area Number |
17K11968
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
Maki Kenshi 九州歯科大学, 歯学部, 教授 (60209400)
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| Co-Investigator(Kenkyū-buntansha) |
松原 琢磨 九州歯科大学, 歯学部, 准教授 (00423137)
古株 彰一郎 九州歯科大学, 歯学部, 教授 (30448899)
青木 和広 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (40272603)
自見 英治郎 九州大学, 歯学研究院, 教授 (40276598)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 骨代謝 / 骨芽細胞 / 破骨細胞 / Bif-1 / Endophilin B1 / SH3GLB1 / Bax相互作用因子 / Bif-1欠損マウス / p130cas / μCT / 骨密度 / ステロイド / p130Cas / 小児 / 骨吸収 |
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| Outline of Final Research Achievements |
Bif-1-deficient (Bif-1 -/- ) mice showed increased trabecular bone volume and trabecular number. Histological analyses indicated that the osteoclast numbers increased in Bif-1 -/- mice. Consistent with the in vivo results, osteoclastogenesis induced by RANKL was accelerated in Bif-1 -/- mice without affecting RANKL-induced activation of RANK downstream signals, such as NF-κB and MAPKs, CD115/RANK expression in osteoclast precursors, osteoclastic bone-resorbing activity and the survival rate. Unexpectedly, both the bone formation rate and osteoblast surface substantially increased in Bif-1 -/- mice. Osteoblastic differentiation and mineralization were enhanced in Bif-1 -/- mice. Finally, bone marrow cells from Bif-1 -/- mice showed a significantly higher colony-forming efficacy, suggesting that cells from Bif-1 -/- mice had higher clonogenicity and self-renewal activity than those from WT mice.
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| Academic Significance and Societal Importance of the Research Achievements |
Bif-1欠損マウスでは骨梁の数と骨量が増加する。そのメカニズムが解明できたことにより、Bif-1をターゲットにした新たな骨代謝治療薬創出につながる可能性がある。
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