Functional role of Transforming growth factor beta during enamel formation

• Project/Area Number: 17K11975
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tsurumi University
• Principal Investigator: ASADA YOSHINOBU 鶴見大学, 歯学部, 教授 (20184145)
• Co-Investigator(Kenkyū-buntansha): 山越 康雄 鶴見大学, 歯学部, 教授 (20182470) ; 山本 竜司 鶴見大学, 歯学部, 講師 (20410053) ; 唐木田 丈夫 鶴見大学, 歯学部, 講師 (40367305) ; 小林 冴子 鶴見大学, 歯学部, 助教 (90804534)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: エナメル質 / 幼若エナメル質 / エナメルタンパク質 / 生理活性物質 / TGF-β / アメロゲニン / エナメルたんぱく質 / TGF-β１

Outline of Final Research Achievements

We focused on transforming growth factor beta (TGF-β), which is found in the process of enamel formation, to elucidate its role and function, and mainly investigated the protein dynamics of TGF-β1. We have elucidated the autocrine mechanism of TGF-β1 by ameloblasts and the complex formation of TGF-β1 with amelogenin fragments in the enamel matrix. We have also identified possible roles for TGF-β in the induction of apoptosis in ameloblasts, endocytosis of amelogenin, and induction of epithelial-mesenchymal transition. Furthermore, we found that the effects on these functions differ among TGF-β isoforms (β1, β2, and β3).

Academic Significance and Societal Importance of the Research Achievements

本研究により、正常なエナメル質形成のメカニズム解明に迫ることのみならず、Amel-TGF-β複合体を応用した新たな歯科再生医療技術の構築が十分に考えられ、学術的および社会的意義は高い。またTGF-βアイソフォーム間による影響を検証することで、形成不全や腫瘍化に至る異常なシグナルについて知る可能性を示唆する。

Research Products

• [Journal Article] TGF-β autocrine signaling at secretory-stage enamel. (2018)
  • Author(s): Kobayashi S, Yamakoshi Y, and Asada Y.
  • Journal Title: J Oral Biosci. Volume: 60, (3) Issue: 3 Pages: 70-75
  • DOI: 10.1016/j.job.2018.05.001
  • Peer Reviewed / Open Access
• [Presentation] Effects of TGF-β isoforms on ameloblasts (2021)
  • Author(s): Y. MIYAKAWA, Y. YAMAKOSHI and Y. ASADA
  • Organizer: JADR Annual Meeting
• [Presentation] Potential Function of TGF-β Isoforms in Maturation-stage Ameloblasts (2019)
  • Author(s): KOBAYASHI, M. OKUBO, R. CHIBA, R. YAMAMOTO, Y. YAMAKOSHI3, and Y. ASADA
  • Organizer: 9７th General Session of the IADR Vancouver, BC, Canada
  • Int'l Joint Research
• [Presentation] TGFβ-アイソフォームによる成熟期エナメルタンパク遺伝子発現の制御 (2019)
  • Author(s): 宮川友里，唐木田丈夫，小林冴子，山越康雄，朝田芳信
  • Organizer: 第61回歯科基礎医学会学術大会