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Strategy for prevention of periodontal disease by controlling protein expressions of junctional epithelium expressed proteins

Research Project

Project/Area Number 17K11994
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Periodontology
Research InstitutionNihon University

Principal Investigator

OGATA Yorimasa  日本大学, 松戸歯学部, 教授 (90204065)

Co-Investigator(Kenkyū-buntansha) 中山 洋平  日本大学, 松戸歯学部, 准教授 (30434088)
高井 英樹  日本大学, 松戸歯学部, 講師 (30453898)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords接合上皮 / アメロチン / ODAM / FDC-SP / 歯周病 / 予防 / 炎症 / TNF-α / 歯肉上皮細胞 / 歯周炎
Outline of Final Research Achievements

Amelotin (AMTN) and Follicular dendritic cell secreted protein (FDC-SP) are secreted proteins expressed in junctional epithelium (JE). To elucidate the transcriptional regulation of AMTN and FDC-SP genes in inflamed gingiva, we have analyzed the effect of TNF-α on the expression of both genes in Ca9-22 human gingival epithelial cells. TNF-α increased AMTN and FDC-SP mRNA and protein levels after 12 h. TNF-α induced luciferase (LUC) activities of human AMTN and FDC-SP gene promoter constructs in Ca9-22 cells. TNF-α induced LUC activities were partially inhibited in the mutation -353AMTN constructs that included mutations in C/EBP1, C/EBP2 and YY1 elements. Transcriptional stimulations by TNF-α were partially inhibited in the -345FDCSP constructs that included mutations in the YY1, GATA, C/EBP2 and C/EBP3. Transcriptional activities induced by TNF-α were inhibited by tyrosine kinase, MEK1/2 and phosphoinositide 3-kinase inhibitors.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果により、接合上皮で発現するアメロチン(AMTN)、ODAMおよびFDC-SPの転写調節機構および相互作用と機能の一部が解明され、接合上皮内での発現制御による歯周病予防に関する基盤データーが得られる可能性がある。その結果、将来的に歯周病の予防だけでなく、「接合上皮発現タンパク質の制御による新たな治療法」につながる可能性があり、意義は大きく、社会的貢献度が高いテーマであると思われる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (37 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (17 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 16 results,  Open Access: 16 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Int'l Joint Research: 9 results)

  • [Int'l Joint Research] Faculty of Dentistry, Univ. of Toronto(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Matrix Dynamics Group/Faculty of Dentsitry/University of Toronto(カナダ)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Matrix Dynamics Group,/Faculty of Dentistry,/University of Toronto(Canada)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Butyric Acid in Saliva of Chronic Periodontitis Patients Induces Transcription of the EBV Lytic Switch Activator BZLF1: A Pilot Study.2020

    • Author(s)
      Koike R, Nodomi K, Watanabe N, Ogata Y, Takeichi O, Takei M, Kaneko T, Tonogi M, Kotani AI, Imai K
    • Journal Title

      in Vivo

      Volume: 34 Issue: 2 Pages: 587-594

    • DOI

      10.21873/invivo.11811

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] How Does Epstein-Barr Virus Contribute to Chronic Periodontitis?2020

    • Author(s)
      Imai K, Ogata Y
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 6 Pages: 1940-1950

    • DOI

      10.3390/ijms21061940

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The periodontopathic bacterium Fusobacterium nucleatum induced proinflammatory cytokine production by human respiratory epithelial cell lines and in the lower respiratory organs in mice.2019

    • Author(s)
      Hayata M, Watanabe N, Tamura M, Kamio N, Tanaka H, Nodomi K, Miya C, Nakayama E, Ueda K, Ogata Y, Imai K
    • Journal Title

      Cellular Physiology and Biochemistry

      Volume: 53 Issue: 1 Pages: 49-61

    • DOI

      10.33594/000000120

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] EBV LMP1 in Gingival Epithelium Potentially Contributes to Human Chronic Periodontitis via Inducible IL8 Production.2019

    • Author(s)
      Watanabe N, Nodomi K, Koike R, Kato A, Takeichi O, Kotani AI, Kaneko T, Sakagami H, Takei M, Ogata Y, Sato S, Imai K
    • Journal Title

      In Vivo

      Volume: 33 Issue: 6 Pages: 1793-1800

    • DOI

      10.21873/invivo.11670

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Negative feedback by SNAI2 regulates TGFβ1-induced amelotin gene transcription in epithelial-mesenchymal transition.2019

    • Author(s)
      Nakayama Y, Tsuruya Y, Noda K, Yamazaki-Takai M, Iwai Y, Ganss B, Ogata Y.
    • Journal Title

      J Cell Physiol.

      Volume: 234 Issue: 7 Pages: 11474-11489

    • DOI

      10.1002/jcp.27804

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Induction of chondrogenic or mesenchymal stem cells from human periodontal ligament cells through inhibition of Twist2 or Klf122019

    • Author(s)
      Takai H, Van Wijnen AJ, Ogata Y.
    • Journal Title

      Journal of Oral Science

      Volume: 61 Issue: 2 Pages: 313-320

    • DOI

      10.2334/josnusd.18-0224

    • NAID

      130007664889

    • ISSN
      1343-4934, 1880-4926
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] IL-1β enhances cell adhesion through laminin 5 and β4 integrin in gingival epithelial cells2019

    • Author(s)
      Mezawa M, Tsuruya Y, Yamazaki-Takai M, Takai H, Nakayama Y, McCulloch CA, Ogata Y.
    • Journal Title

      Journal of Oral Science

      Volume: 61 Issue: 4 Pages: 491-497

    • DOI

      10.2334/josnusd.18-0434

    • NAID

      130007751577

    • ISSN
      1343-4934, 1880-4926
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cynaropicrin from Cynara scolymus L. suppresses Porphyromonas gingivalis LPS-induced production of inflammatory cytokines in human gingival fibroblasts and RANKL-induced osteoclast differentiation in RAW264.7 cells2018

    • Author(s)
      Hayata Mayumi、Watanabe Norihisa、Kamio Noriaki、Tamura Muneaki、Nodomi Keiko、Tanaka Kiyotaka、Iddamalgoda Arunasiri、Tsuda Hiromasa、Ogata Yorimasa、Sato Shuichi、Ueda Kouichiro、Imai Kenichi
    • Journal Title

      Journal of Natural Medicines

      Volume: 73 Issue: 1 Pages: 114-123

    • DOI

      10.1007/s11418-018-1250-6

    • NAID

      40021790096

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] IL-1β and TNF-α regulate mouse amelotin gene transcription in gingival epithelial cells.2018

    • Author(s)
      Noda K, Yamazaki M, Iwai Y, Matsui S, Kato A, Takai H, Nakayama Y, Ogata Y
    • Journal Title

      J Oral Sci

      Volume: 60 Issue: 3 Pages: 388-398

    • DOI

      10.2334/josnusd.17-0388

    • NAID

      130007485938

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of interleukin-1β on human follicular dendritic cell-secreted protein gene expression in periodontal ligament cells2018

    • Author(s)
      Iwai Y, Noda K, Yamazaki M, Mezawa M, Takai H, Nakayama Y, Kitagawa M, Takata T, Ogata Y
    • Journal Title

      Journal of Oral Science

      Volume: 60 Issue: 4 Pages: 601-610

    • DOI

      10.2334/josnusd.17-0473

    • NAID

      130007553038

    • ISSN
      1343-4934, 1880-4926
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] TGFβ1-induced Amelotin gene expression is downregulated by Bax expression in mouse gingival epithelial cells2018

    • Author(s)
      Nakayama Y, Matsui S, Noda K, Yamazaki M, Iwai Y, Ganss B, Ogata Y
    • Journal Title

      Journal of Oral Science

      Volume: 60 Issue: 2 Pages: 232-241

    • DOI

      10.2334/josnusd.17-0271

    • NAID

      130007398293

    • ISSN
      1343-4934, 1880-4926
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcriptional regulation of human amelotin gene by interleukin-1β.2018

    • Author(s)
      Yamazaki M, Mezawa M, Noda K, Iwai Y, Matsui S, Takai H, Nakayama Y, Ogata Y
    • Journal Title

      FEBS Open Bio

      Volume: 8 Issue: 6 Pages: 974-985

    • DOI

      10.1002/2211-5463.12434

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] MiR-200b attenuates IL-6 production through IKKβ and ZEB1 in human gingival fibroblasts.2018

    • Author(s)
      Matsui S, Zhou L, Nakayama Y, Mezawa M, Kato A, Suzuki N, Tanabe N, Nakayama T, Suzuki Y, Kamio N, Takai H, Ogata Y
    • Journal Title

      Inflamm Res

      Volume: 67 Issue: 11-12 Pages: 965-973

    • DOI

      10.1007/s00011-018-1192-1

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] C/EBPβ and YY1 bind and interact with Smad3 to modulate lipopolysaccharide-induced amelotin gene transcription in mouse gingival epithelial cells2018

    • Author(s)
      Nakayama Yohei、Kobayashi Ryoki、Iwai Yasunobu、Noda Keisuke、Yamazaki Mizuho、Kurita-Ochiai Tomoko、Yoshimura Atsutoshi、Ganss Bernhard、Ogata Yorimasa
    • Journal Title

      FEBS Open Bio

      Volume: 9 Issue: 2 Pages: 276-290

    • DOI

      10.1002/2211-5463.12566

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Tumor necrosis factor-α stimulates human amelotin gene transcription in gingival epithelial cells.2018

    • Author(s)
      Yamazaki M, Iwai Y, Noda K, Matsui S, Kato A, Takai H, Nakayama Y, Ogata Y
    • Journal Title

      Inflammation Res

      Volume: 67 Issue: 4 Pages: 351-361

    • DOI

      10.1007/s00011-017-1126-3

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tumor necrosis factor-α regulates human follicular dendritic cell-secreted protein gene transcription in gingival epithelial cells.2018

    • Author(s)
      Iwai Y, Noda K, Yamazaki M, Kato A, Mezawa M, Takai H, Nakayama Y, Ogata Y
    • Journal Title

      Genes Cells

      Volume: 23 Issue: 3 Pages: 161-171

    • DOI

      10.1111/gtc.12561

    • Related Report
      2017 Research-status Report
    • Open Access
  • [Journal Article] Localization and expression pattern of amelotin, odontogenic ameloblast-associated protein and follicular dendritic cell-secreted protein in the junctional epithelium of inflamed gingiva.2016

    • Author(s)
      Nakayama Y, Kobayashi R, Matsui S, Matsumura H, Iwai Y, Noda K, Yamazaki M, Kurita-Ochiai T, Yoshimura A, Shinomura T, Ganss B, Ogata Y.
    • Journal Title

      Odontology

      Volume: - Issue: 3 Pages: 329-337

    • DOI

      10.1007/s10266-016-0277-y

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] SNAI2は上皮間葉移行におけるTGFβ1誘導AMTN遺伝子の発現を負に調節する2019

    • Author(s)
      中山洋平、鶴屋祐人、能田佳祐、高井瑞穂、岩井泰伸、小方頼昌
    • Organizer
      第62回春季日本歯周病学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 炎症性サイトカインは歯肉上皮細胞での細胞-基質接着分子の構成に影響を及ぼす2019

    • Author(s)
      目澤優、鶴屋祐人、横山正樹、小方頼昌
    • Organizer
      第62回秋季日本歯周病学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 炎症性サイトカインがエクソソームに及ぼす影響2019

    • Author(s)
      山口亜利彩、鶴屋祐人、能田佳祐、高井瑞穂、目澤優、高井英樹、中山洋平、小方頼昌
    • Organizer
      第62回秋季日本歯周病学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 炎症性サイトカインによるODAM転写調節機構の解析2019

    • Author(s)
      鶴屋祐人、山口亜利彩、能田佳祐、高井瑞穂、目澤優、高井英樹、中山洋平、小方頼昌
    • Organizer
      第62回秋季日本歯周病学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Twist2またはKif12阻害によるヒト歯根膜細胞からの軟骨形成または間葉系幹細胞への誘導2019

    • Author(s)
      高井英樹、小方頼昌
    • Organizer
      第62回秋季日本歯周病学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] miR-200bはヒト歯肉上皮細胞におけるアメロチン遺伝子発現を抑制する2019

    • Author(s)
      高井瑞穂、能田佳祐、岩井泰伸、目澤優、高井英樹、中山洋平、小方頼昌
    • Organizer
      第62回秋季日本歯周病学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] miR-150はヒト歯肉上皮細胞においてMKP-5を介してマウスアメロチン遺伝子発現を調節する2019

    • Author(s)
      能田佳祐、高井瑞穂、岩井泰伸、目澤優、高井英樹、中山洋平、小方頼昌
    • Organizer
      第62回秋季日本歯周病学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ポケット探針を用いたプロービングポケット深さと歯周ポケット測定器での測定値の比較2019

    • Author(s)
      吉野祥一、小方頼昌
    • Organizer
      第62回秋季日本歯周病学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] MiR-200b inhibits IL-6 expression through IKKβ and ZEB1 in human gingival fibroblasts.2019

    • Author(s)
      Ogata Y, Nakayama Y, Mezawa M, Suzuki N, Tanabe N, Nakayama T, Suzuki Y, Kamio N, Kato A, Takai H, Matsui S
    • Organizer
      13th Asian Pacific Society of Periodontology Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] MicroRNA expression in inflamed gingival tissues and effects of miR-200b on the expression of Interleukin-6 in human gingival fibroblasts.2018

    • Author(s)
      Ogata Y, Nakayama Y, Kato A, Mezawa M, Suzuki N, Tanabe N, Nakayama T, Suzuki Y, Kamio N, Takai H, Matsui S
    • Organizer
      EuroPerio9
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Prevalence and Risk Factors for Peri-Implant Diseases: A Cross-Sectional Multi-Center Study by Periodontal Specialists.2018

    • Author(s)
      Ogata Y
    • Organizer
      104th Annual Meeting of American Academy of Periodontology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of TNF-α on the expression human FDC-SP gene in gingival epithelial cells.2017

    • Author(s)
      Iwai Y, Noda K, Yamazaki M, Matsui S, Kato A, Takai H, Nakayama Y, Ogata Y
    • Organizer
      12th International Conference on the Chemistry and Biology of Mineralized Tissues
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Comparative study of transcriptional regulation of bone sialoprotein in osteoblast- and cementoblast-like cells.2017

    • Author(s)
      Noda K, Yamazaki M, Iwai Y, Matsui S, Kato A, Takai H, Nakayama Y, Ogata Y
    • Organizer
      12th International Conference on the Chemistry and Biology of Mineralized Tissues
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Inflammatory cytokines regulate human AMTN gene transcription in gingival epithelial cells.2017

    • Author(s)
      Yamazaki M, Iwai Y, Noda K, Matsui S, Kato A, Takai H, Nakayama Y, Ogata Y
    • Organizer
      12th International Conference on the Chemistry and Biology of Mineralized Tissues
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Amelotin gene expression is upregulated at the initiation of apoptosis induced by TGFβ1 in gingival epithelial cells.2017

    • Author(s)
      Nakayama Y, Matsui S, Noda K, Yamazaki M, Iwai Y, Matsumura H, Ganss B, Ogata Y
    • Organizer
      12th International Conference on the Chemistry and Biology of Mineralized Tissues
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of miRNAs on the expressions of inflammatory cytokines in human gingival fibroblasts.2017

    • Author(s)
      Ogata Y, Matsui S, Suzuki N, Nakayama T, Tanabe N, Suzuki Y, Kamio N, Kato A, Mezawa M, Nakayama Y
    • Organizer
      American Academy of Periodontology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] MicroRNA expression in inflamed gingival tissues and the effects of miR-223 on expression of inflammatory cytokines in human gingival fibroblasts.2017

    • Author(s)
      Ogata Y, Nakayama Y, Kato A, Mezawa M, Suzuki N, Tanabe N, Nakayama T, Suzuki Y, Kamio N, Takai H, Matsui S
    • Organizer
      12th Asian Pacific Society of Periodontology Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2021-02-19  

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