Strategy for prevention of periodontal disease by controlling protein expressions of junctional epithelium expressed proteins
Project/Area Number |
17K11994
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Periodontology
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Research Institution | Nihon University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
中山 洋平 日本大学, 松戸歯学部, 准教授 (30434088)
高井 英樹 日本大学, 松戸歯学部, 講師 (30453898)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 接合上皮 / アメロチン / ODAM / FDC-SP / 歯周病 / 予防 / 炎症 / TNF-α / 歯肉上皮細胞 / 歯周炎 |
Outline of Final Research Achievements |
Amelotin (AMTN) and Follicular dendritic cell secreted protein (FDC-SP) are secreted proteins expressed in junctional epithelium (JE). To elucidate the transcriptional regulation of AMTN and FDC-SP genes in inflamed gingiva, we have analyzed the effect of TNF-α on the expression of both genes in Ca9-22 human gingival epithelial cells. TNF-α increased AMTN and FDC-SP mRNA and protein levels after 12 h. TNF-α induced luciferase (LUC) activities of human AMTN and FDC-SP gene promoter constructs in Ca9-22 cells. TNF-α induced LUC activities were partially inhibited in the mutation -353AMTN constructs that included mutations in C/EBP1, C/EBP2 and YY1 elements. Transcriptional stimulations by TNF-α were partially inhibited in the -345FDCSP constructs that included mutations in the YY1, GATA, C/EBP2 and C/EBP3. Transcriptional activities induced by TNF-α were inhibited by tyrosine kinase, MEK1/2 and phosphoinositide 3-kinase inhibitors.
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Academic Significance and Societal Importance of the Research Achievements |
本研究の成果により、接合上皮で発現するアメロチン(AMTN)、ODAMおよびFDC-SPの転写調節機構および相互作用と機能の一部が解明され、接合上皮内での発現制御による歯周病予防に関する基盤データーが得られる可能性がある。その結果、将来的に歯周病の予防だけでなく、「接合上皮発現タンパク質の制御による新たな治療法」につながる可能性があり、意義は大きく、社会的貢献度が高いテーマであると思われる。
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Report
(4 results)
Research Products
(37 results)
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[Journal Article] The periodontopathic bacterium Fusobacterium nucleatum induced proinflammatory cytokine production by human respiratory epithelial cell lines and in the lower respiratory organs in mice.2019
Author(s)
Hayata M, Watanabe N, Tamura M, Kamio N, Tanaka H, Nodomi K, Miya C, Nakayama E, Ueda K, Ogata Y, Imai K
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Journal Title
Cellular Physiology and Biochemistry
Volume: 53
Issue: 1
Pages: 49-61
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] EBV LMP1 in Gingival Epithelium Potentially Contributes to Human Chronic Periodontitis via Inducible IL8 Production.2019
Author(s)
Watanabe N, Nodomi K, Koike R, Kato A, Takeichi O, Kotani AI, Kaneko T, Sakagami H, Takei M, Ogata Y, Sato S, Imai K
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Journal Title
In Vivo
Volume: 33
Issue: 6
Pages: 1793-1800
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] MiR-200b attenuates IL-6 production through IKKβ and ZEB1 in human gingival fibroblasts.2018
Author(s)
Matsui S, Zhou L, Nakayama Y, Mezawa M, Kato A, Suzuki N, Tanabe N, Nakayama T, Suzuki Y, Kamio N, Takai H, Ogata Y
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Journal Title
Inflamm Res
Volume: 67
Issue: 11-12
Pages: 965-973
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Localization and expression pattern of amelotin, odontogenic ameloblast-associated protein and follicular dendritic cell-secreted protein in the junctional epithelium of inflamed gingiva.2016
Author(s)
Nakayama Y, Kobayashi R, Matsui S, Matsumura H, Iwai Y, Noda K, Yamazaki M, Kurita-Ochiai T, Yoshimura A, Shinomura T, Ganss B, Ogata Y.
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Journal Title
Odontology
Volume: -
Issue: 3
Pages: 329-337
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] MiR-200b inhibits IL-6 expression through IKKβ and ZEB1 in human gingival fibroblasts.2019
Author(s)
Ogata Y, Nakayama Y, Mezawa M, Suzuki N, Tanabe N, Nakayama T, Suzuki Y, Kamio N, Kato A, Takai H, Matsui S
Organizer
13th Asian Pacific Society of Periodontology Meeting
Related Report
Int'l Joint Research
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[Presentation] MicroRNA expression in inflamed gingival tissues and effects of miR-200b on the expression of Interleukin-6 in human gingival fibroblasts.2018
Author(s)
Ogata Y, Nakayama Y, Kato A, Mezawa M, Suzuki N, Tanabe N, Nakayama T, Suzuki Y, Kamio N, Takai H, Matsui S
Organizer
EuroPerio9
Related Report
Int'l Joint Research
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[Presentation] MicroRNA expression in inflamed gingival tissues and the effects of miR-223 on expression of inflammatory cytokines in human gingival fibroblasts.2017
Author(s)
Ogata Y, Nakayama Y, Kato A, Mezawa M, Suzuki N, Tanabe N, Nakayama T, Suzuki Y, Kamio N, Takai H, Matsui S
Organizer
12th Asian Pacific Society of Periodontology Meeting
Related Report
Int'l Joint Research